Biochemical and structural characterization of the human gut microbiome metallopeptidase IgAse provides insight into its unique specificity for the Fab’ region of IgA1 and IgA2

Juan Sebastián Ramírez-Larrota; Pauline Juyoux; Pablo Guerra; Ulrich Eckhard; F. Xavier Gomis-Rüth

doi:10.1371/journal.ppat.1013292

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Peer-reviewed

Research Article

Biochemical and structural characterization of the human gut microbiome metallopeptidase IgAse provides insight into its unique specificity for the F_ab’ region of IgA1 and IgA2

Juan Sebastián Ramírez-Larrota,

Roles Formal analysis, Investigation, Methodology

Affiliations Proteolysis Laboratory, Department of Structural and Molecular Biology, Molecular Biology Institute of Barcelona (CSIC), Barcelona Science Park, Barcelona, Catalonia, Spain, Synthetic Structural Biology Group, Department of Structural and Molecular Biology, Molecular Biology Institute of Barcelona (CSIC), Barcelona Science Park, Barcelona, Catalonia, Spain, Graduate Program in Biotechnology, Faculty of Pharmacy and Food Sciences, University of Barcelona, Barcelona, Catalonia, Spain
⨯
Pauline Juyoux,

Roles Investigation, Methodology

Affiliation Methods and Electron Microscopy Group, University Grenoble-Alpes, CNRS, CEA, EMBL, ISBG, Grenoble, France
⨯
Pablo Guerra,

Roles Data curation, Formal analysis, Investigation, Methodology

Affiliation Cryo-Electron Microscopy Platform, Molecular Biology Institute of Barcelona (CSIC) and Joint Electron Microscopy Center at ALBA (JEMCA), Cerdanyola del Vallès, Catalonia, Spain
⨯
Ulrich Eckhard ,

Roles Conceptualization, Data curation, Formal analysis, Funding acquisition, Investigation, Supervision, Validation, Writing – review & editing

* E-mail: ueccri@ibmb.csic.es (UE); xgrcri@ibmb.csic.es (FXGR)

Affiliation Synthetic Structural Biology Group, Department of Structural and Molecular Biology, Molecular Biology Institute of Barcelona (CSIC), Barcelona Science Park, Barcelona, Catalonia, Spain
⨯
F. Xavier Gomis-Rüth

Roles Conceptualization, Data curation, Formal analysis, Funding acquisition, Investigation, Project administration, Resources, Supervision, Validation, Visualization, Writing – original draft

* E-mail: ueccri@ibmb.csic.es (UE); xgrcri@ibmb.csic.es (FXGR)

Affiliation Proteolysis Laboratory, Department of Structural and Molecular Biology, Molecular Biology Institute of Barcelona (CSIC), Barcelona Science Park, Barcelona, Catalonia, Spain

https://orcid.org/0000-0002-6848-6874

⨯

Biochemical and structural characterization of the human gut microbiome metallopeptidase IgAse provides insight into its unique specificity for the F_ab’ region of IgA1 and IgA2

Juan Sebastián Ramírez-Larrota,
Pauline Juyoux,
Pablo Guerra,
Ulrich Eckhard,
F. Xavier Gomis-Rüth

Published: July 8, 2025
https://doi.org/10.1371/journal.ppat.1013292

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