Unique growth and morphology properties of Clade 5 Clostridioides difficile strains revealed by single-cell time-lapse microscopy

• John W. Ribis ,

  Contributed equally to this work with: John W. Ribis, César Nieto

  Roles Data curation, Formal analysis, Investigation, Methodology, Resources, Software, Supervision, Validation, Visualization, Writing – original draft, Writing – review & editing

  ¤ Current address: Nikon Instruments

  Affiliations Department of Molecular Biology and Microbiology, Tufts University School of Medicine, Boston, Massachusetts, United States of America, Graduate School of Biomedical Sciences, Tufts University School of Medicine, Boston, Massachusetts, United States of America

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• César Nieto ,

  Contributed equally to this work with: John W. Ribis, César Nieto

  Roles Formal analysis, Methodology, Software, Writing – original draft

  Affiliation Department of Electrical and Computer Engineering, University of Delaware, Newark, Delaware, United States of America

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• Nicholas V. DiBenedetto,

  Roles Formal analysis, Investigation, Methodology, Writing – review & editing

  Affiliations Department of Molecular Biology and Microbiology, Tufts University School of Medicine, Boston, Massachusetts, United States of America, Graduate School of Biomedical Sciences, Tufts University School of Medicine, Boston, Massachusetts, United States of America

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• Anchal Mehra,

  Roles Formal analysis, Investigation, Writing – review & editing

  Affiliation Department of Microbiology and Immunology, University of North Carolina at Chapel Hill School of Medicine, Chapel Hill, North Carolina, United States of America

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• Pola Kuhn,

  Roles Investigation, Visualization, Writing – review & editing

  Affiliations Department of Molecular Biology and Microbiology, Tufts University School of Medicine, Boston, Massachusetts, United States of America, Graduate School of Biomedical Sciences, Tufts University School of Medicine, Boston, Massachusetts, United States of America

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• Qiwen Dong,

  Roles Formal analysis, Investigation, Writing – review & editing

  Affiliation Department of Molecular Biology and Microbiology, Tufts University School of Medicine, Boston, Massachusetts, United States of America

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• Irene Nagawa,

  Roles Investigation

  Affiliation Department of Molecular Biology and Microbiology, Tufts University School of Medicine, Boston, Massachusetts, United States of America

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• Imane El Meouche,

  Roles Methodology, Writing – review & editing

  Affiliation Department of Biomedical Engineering, Tufts University School of Engineering, Medford, Massachusetts, United States of America

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• Bree B. Aldridge,

  Roles Conceptualization, Funding acquisition, Methodology, Writing – review & editing

  Affiliations Department of Molecular Biology and Microbiology, Tufts University School of Medicine, Boston, Massachusetts, United States of America, INSERM, Université Paris Cité, Université Sorbonne Paris Nord, Inserm, IAME, Paris, France

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• Mary J. Dunlop,

  Roles Funding acquisition, Methodology, Supervision, Writing – review & editing

  Affiliation Biomedical Engineering, Boston University, Boston, Massachusetts United States of America

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• Rita Tamayo,

  Roles Data curation, Formal analysis, Funding acquisition, Investigation, Resources, Supervision, Writing – review & editing

  Affiliation Department of Microbiology and Immunology, University of North Carolina at Chapel Hill School of Medicine, Chapel Hill, North Carolina, United States of America

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• Abhyudai Singh,

  Roles Data curation, Funding acquisition, Validation, Writing – review & editing

  Affiliation Department of Electrical and Computer Engineering, University of Delaware, Newark, Delaware, United States of America

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• Aimee Shen

  Roles Formal analysis, Funding acquisition, Investigation, Methodology, Project administration, Supervision, Visualization, Writing – original draft, Writing – review & editing

  * E-mail: aimee.shen@tufts.edu

  Affiliation Department of Molecular Biology and Microbiology, Tufts University School of Medicine, Boston, Massachusetts, United States of America

  https://orcid.org/0000-0002-9786-5742

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