Structural basis and mode of action for two broadly neutralizing nanobodies targeting the highly conserved spike stem-helix of sarbecoviruses including SARS-CoV-2 and its variants

• Liyan Guo,

  Roles Formal analysis, Funding acquisition, Investigation, Methodology, Validation, Visualization, Writing – original draft, Writing – review & editing

  Affiliations Department of Emergency Medicine, State Key Laboratory of Biotherapy, West China Hospital, Sichuan University, Chengdu, Sichuan, China, Department of Hematology, West China Hospital, Sichuan University, Chengdu, Sichuan, China

  ⨯
• Zimin Chen,

  Roles Formal analysis, Funding acquisition, Investigation, Methodology, Validation

  Affiliation Department of Emergency Medicine, State Key Laboratory of Biotherapy, West China Hospital, Sichuan University, Chengdu, Sichuan, China

  ⨯
• Sheng Lin,

  Roles Formal analysis, Funding acquisition, Investigation, Methodology, Validation, Visualization, Writing – review & editing

  Affiliation Department of Emergency Medicine, State Key Laboratory of Biotherapy, West China Hospital, Sichuan University, Chengdu, Sichuan, China

  ⨯
• Fanli Yang,

  Roles Formal analysis, Funding acquisition, Investigation, Methodology, Validation

  Affiliation Department of Emergency Medicine, State Key Laboratory of Biotherapy, West China Hospital, Sichuan University, Chengdu, Sichuan, China

  ⨯
• Jing Yang,

  Roles Formal analysis, Investigation, Methodology, Visualization

  Affiliation Department of Emergency Medicine, State Key Laboratory of Biotherapy, West China Hospital, Sichuan University, Chengdu, Sichuan, China

  ⨯
• Lingling Wang,

  Roles Investigation, Methodology, Visualization

  Affiliation Department of Emergency Medicine, State Key Laboratory of Biotherapy, West China Hospital, Sichuan University, Chengdu, Sichuan, China

  ⨯
• Xindan Zhang,

  Roles Investigation, Methodology

  Affiliation Department of Emergency Medicine, State Key Laboratory of Biotherapy, West China Hospital, Sichuan University, Chengdu, Sichuan, China

  ⨯
• Xin Yuan,

  Roles Investigation, Methodology

  Affiliation Department of Emergency Medicine, State Key Laboratory of Biotherapy, West China Hospital, Sichuan University, Chengdu, Sichuan, China

  ⨯
• Bin He,

  Roles Investigation, Methodology, Resources, Writing – review & editing

  Affiliation Department of Emergency Medicine, State Key Laboratory of Biotherapy, West China Hospital, Sichuan University, Chengdu, Sichuan, China

  ⨯
• Yu Cao,

  Roles Investigation, Methodology, Resources, Writing – review & editing

  Affiliations Department of Emergency Medicine, State Key Laboratory of Biotherapy, West China Hospital, Sichuan University, Chengdu, Sichuan, China, Disaster Medicine Center, West China Hospital, Sichuan University, Chengdu, Sichuan, China

  ⨯
• Jian Li,

  Roles Investigation, Methodology, Resources, Writing – review & editing

  Affiliation School of Basic Medical Sciences, Chengdu University, Chengdu, Sichuan China

  ⨯
• Qi Zhao,

  Roles Investigation, Methodology, Resources, Writing – review & editing

  Affiliation College of Food and Biological Engineering, Chengdu University, Chengdu, Sichuan China

  ⨯
• Guangwen Lu

  Roles Conceptualization, Formal analysis, Funding acquisition, Resources, Supervision, Writing – original draft, Writing – review & editing

  * E-mail: lugw@scu.edu.cn

  Affiliation Department of Emergency Medicine, State Key Laboratory of Biotherapy, West China Hospital, Sichuan University, Chengdu, Sichuan, China

  https://orcid.org/0000-0001-7568-592X

  ⨯