Super broad and protective nanobodies against Sarbecoviruses including SARS-CoV-1 and the divergent SARS-CoV-2 subvariant KP.3.1.1

Haodi Dong; Runhong Zhou; Jing Chen; Jing Wei; Zimeng Wei; Ziqing Yang; Kun Zhu; Yufan Yang; Qianqian Yang; Na Liu; Yuting Chen; Yuhan Wu; Yan Liang; Yige Zeng; Qile Guo; Mingxi Li; Sisi Shan; Han Wang; Mengyue Niu; Isabella Yunfei Zeng; Xuanling Shi; Qi Zhang; Xinquan Wang; Zhiwei Chen; Linqi Zhang

doi:10.1371/journal.ppat.1012625

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Open Access

Peer-reviewed

Research Article

Super broad and protective nanobodies against Sarbecoviruses including SARS-CoV-1 and the divergent SARS-CoV-2 subvariant KP.3.1.1

Haodi Dong ,

Contributed equally to this work with: Haodi Dong, Runhong Zhou, Jing Chen, Jing Wei

Roles Conceptualization, Data curation, Formal analysis, Investigation, Methodology, Project administration, Resources, Validation, Visualization, Writing – original draft

Affiliation Comprehensive AIDS Research Center, Pandemic Research Alliance Unit, Center for Infection Biology, School of Basic Medical Sciences, Tsinghua Medicine, Tsinghua University, Beijing, China
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Runhong Zhou ,

Contributed equally to this work with: Haodi Dong, Runhong Zhou, Jing Chen, Jing Wei

Roles Methodology, Resources, Writing – original draft

Affiliations AIDS Institute, School of Clinical Medicine, Li Ka Shing Faculty of Medicine, The University of Hong Kong, Pokfulam, Hong Kong Special Administrative Region, People’s Republic of China, Department of Microbiology, School of Clinical Medicine, Li Ka Shing Faculty of Medicine, The University of Hong Kong, Pokfulam, Hong Kong Special Administrative Region, People’s Republic of China, Centre for Virology, Vaccinology and Therapeutics, Hong Kong Science and Technology Park, Hong Kong Special Administrative Region, People’s Republic of China
⨯
Jing Chen ,

Contributed equally to this work with: Haodi Dong, Runhong Zhou, Jing Chen, Jing Wei

Roles Data curation, Formal analysis, Investigation, Methodology, Resources, Visualization, Writing – original draft

Affiliation The Ministry of Education Key Laboratory of Protein Science, Beijing Advanced Innovation Center for Structural Biology, Beijing Frontier Research Center for Biological Structure, Collaborative Innovation Center for Biotherapy, School of Life Sciences, Tsinghua University, Beijing, China
⨯
Jing Wei ,

Contributed equally to this work with: Haodi Dong, Runhong Zhou, Jing Chen, Jing Wei

Roles Formal analysis, Investigation, Methodology, Writing – original draft

Affiliation Comprehensive AIDS Research Center, Pandemic Research Alliance Unit, Center for Infection Biology, School of Basic Medical Sciences, Tsinghua Medicine, Tsinghua University, Beijing, China
⨯
Zimeng Wei,

Roles Investigation

Affiliation Comprehensive AIDS Research Center, Pandemic Research Alliance Unit, Center for Infection Biology, School of Basic Medical Sciences, Tsinghua Medicine, Tsinghua University, Beijing, China
⨯
Ziqing Yang,

Roles Formal analysis, Investigation, Software, Visualization

Affiliation Comprehensive AIDS Research Center, Pandemic Research Alliance Unit, Center for Infection Biology, School of Basic Medical Sciences, Tsinghua Medicine, Tsinghua University, Beijing, China
⨯
Kun Zhu,

Roles Investigation

Affiliation Comprehensive AIDS Research Center, Pandemic Research Alliance Unit, Center for Infection Biology, School of Basic Medical Sciences, Tsinghua Medicine, Tsinghua University, Beijing, China
⨯
Yufan Yang,

Roles Investigation

Affiliation Comprehensive AIDS Research Center, Pandemic Research Alliance Unit, Center for Infection Biology, School of Basic Medical Sciences, Tsinghua Medicine, Tsinghua University, Beijing, China
⨯
Qianqian Yang,

Roles Formal analysis, Investigation, Software, Visualization

Affiliation Comprehensive AIDS Research Center, Pandemic Research Alliance Unit, Center for Infection Biology, School of Basic Medical Sciences, Tsinghua Medicine, Tsinghua University, Beijing, China
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Na Liu,

Roles Investigation

Affiliation AIDS Institute, School of Clinical Medicine, Li Ka Shing Faculty of Medicine, The University of Hong Kong, Pokfulam, Hong Kong Special Administrative Region, People’s Republic of China
⨯
Yuting Chen,

Roles Investigation

Affiliation AIDS Institute, School of Clinical Medicine, Li Ka Shing Faculty of Medicine, The University of Hong Kong, Pokfulam, Hong Kong Special Administrative Region, People’s Republic of China
⨯
Yuhan Wu,

Roles Investigation

Affiliation Comprehensive AIDS Research Center, Pandemic Research Alliance Unit, Center for Infection Biology, School of Basic Medical Sciences, Tsinghua Medicine, Tsinghua University, Beijing, China
⨯
Yan Liang,

Roles Investigation

Affiliation Comprehensive AIDS Research Center, Pandemic Research Alliance Unit, Center for Infection Biology, School of Basic Medical Sciences, Tsinghua Medicine, Tsinghua University, Beijing, China
⨯
Yige Zeng,

Roles Investigation

Affiliation Comprehensive AIDS Research Center, Pandemic Research Alliance Unit, Center for Infection Biology, School of Basic Medical Sciences, Tsinghua Medicine, Tsinghua University, Beijing, China
⨯
Qile Guo,

Roles Investigation

Affiliation Comprehensive AIDS Research Center, Pandemic Research Alliance Unit, Center for Infection Biology, School of Basic Medical Sciences, Tsinghua Medicine, Tsinghua University, Beijing, China
⨯
Mingxi Li,

Roles Resources

Affiliation Comprehensive AIDS Research Center, Pandemic Research Alliance Unit, Center for Infection Biology, School of Basic Medical Sciences, Tsinghua Medicine, Tsinghua University, Beijing, China
⨯
Sisi Shan,

Roles Methodology

Affiliation Comprehensive AIDS Research Center, Pandemic Research Alliance Unit, Center for Infection Biology, School of Basic Medical Sciences, Tsinghua Medicine, Tsinghua University, Beijing, China
⨯
Han Wang,

Roles Methodology

Affiliation Comprehensive AIDS Research Center, Pandemic Research Alliance Unit, Center for Infection Biology, School of Basic Medical Sciences, Tsinghua Medicine, Tsinghua University, Beijing, China
⨯
Mengyue Niu,

Roles Investigation

Affiliation Comprehensive AIDS Research Center, Pandemic Research Alliance Unit, Center for Infection Biology, School of Basic Medical Sciences, Tsinghua Medicine, Tsinghua University, Beijing, China
⨯
Isabella Yunfei Zeng,

Roles Investigation

Affiliation School of Engineering, Massachusetts Institute of Technology, Cambridge, Massachusetts, United States
⨯
Xuanling Shi,

Roles Funding acquisition

Affiliation Comprehensive AIDS Research Center, Pandemic Research Alliance Unit, Center for Infection Biology, School of Basic Medical Sciences, Tsinghua Medicine, Tsinghua University, Beijing, China
⨯
Qi Zhang,

Roles Funding acquisition

Affiliation Comprehensive AIDS Research Center, Pandemic Research Alliance Unit, Center for Infection Biology, School of Basic Medical Sciences, Tsinghua Medicine, Tsinghua University, Beijing, China
⨯
Xinquan Wang ,

Roles Funding acquisition, Writing – review & editing

* E-mail: xinquanwang@tsinghua.edu.cn (XW); zchenai@hku.hk (ZC); zhanglinqi@tsinghua.edu.cn (LZ)

Affiliation The Ministry of Education Key Laboratory of Protein Science, Beijing Advanced Innovation Center for Structural Biology, Beijing Frontier Research Center for Biological Structure, Collaborative Innovation Center for Biotherapy, School of Life Sciences, Tsinghua University, Beijing, China
⨯
Zhiwei Chen ,

Roles Conceptualization, Funding acquisition

* E-mail: xinquanwang@tsinghua.edu.cn (XW); zchenai@hku.hk (ZC); zhanglinqi@tsinghua.edu.cn (LZ)

Affiliations AIDS Institute, School of Clinical Medicine, Li Ka Shing Faculty of Medicine, The University of Hong Kong, Pokfulam, Hong Kong Special Administrative Region, People’s Republic of China, Department of Microbiology, School of Clinical Medicine, Li Ka Shing Faculty of Medicine, The University of Hong Kong, Pokfulam, Hong Kong Special Administrative Region, People’s Republic of China, Centre for Virology, Vaccinology and Therapeutics, Hong Kong Science and Technology Park, Hong Kong Special Administrative Region, People’s Republic of China
⨯
[ ... ],
Linqi Zhang

Roles Conceptualization, Data curation, Funding acquisition, Project administration, Supervision, Validation, Writing – original draft, Writing – review & editing

* E-mail: xinquanwang@tsinghua.edu.cn (XW); zchenai@hku.hk (ZC); zhanglinqi@tsinghua.edu.cn (LZ)

Affiliations Comprehensive AIDS Research Center, Pandemic Research Alliance Unit, Center for Infection Biology, School of Basic Medical Sciences, Tsinghua Medicine, Tsinghua University, Beijing, China, Institute of Biopharmaceutical and Health Engineering, Tsinghua Shenzhen International Graduate School, Tsinghua University, Shenzhen, China, Institute of Biomedical Health Technology and Engineering, Shenzhen Bay Laboratory, Shenzhen, China

https://orcid.org/0000-0003-4931-509X

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Super broad and protective nanobodies against Sarbecoviruses including SARS-CoV-1 and the divergent SARS-CoV-2 subvariant KP.3.1.1

Haodi Dong,
Runhong Zhou,
Jing Chen,
Jing Wei,
Zimeng Wei,
Ziqing Yang,
Kun Zhu,
Yufan Yang,
Qianqian Yang,
Na Liu

Published: November 11, 2024
https://doi.org/10.1371/journal.ppat.1012625

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