Purified CDT toxins and a clean deletion within the CDT locus provide novel insights into the contribution of binary toxin in cellular inflammation and Clostridioides difficile infection

• Kateryna Nabukhotna,

  Roles Conceptualization, Data curation, Formal analysis, Investigation, Methodology, Resources, Validation, Visualization, Writing – original draft, Writing – review & editing

  Affiliation Department of Biochemistry, Vanderbilt University, Nashville, Tennessee, United States of America

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• Shannon L. Kordus,

  Roles Resources, Validation, Writing – review & editing

  Affiliation Department of Pathology, Microbiology, and Immunology, Vanderbilt University Medical Center, Nashville, Tennessee, United States of America

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• John A. Shupe,

  Roles Investigation, Methodology, Resources

  Affiliation Department of Pathology, Microbiology, and Immunology, Vanderbilt University Medical Center, Nashville, Tennessee, United States of America

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• Rubén Cano Rodríguez,

  Roles Investigation

  Affiliation Department of Pathology, Microbiology, and Immunology, Vanderbilt University Medical Center, Nashville, Tennessee, United States of America

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• Anna Smith,

  Roles Resources

  Affiliation Department of Pathology, Microbiology, and Immunology, Vanderbilt University Medical Center, Nashville, Tennessee, United States of America

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• Julia K. Bohannon,

  Roles Methodology, Supervision, Writing – review & editing

  Affiliations Department of Pathology, Microbiology, and Immunology, Vanderbilt University Medical Center, Nashville, Tennessee, United States of America, Department of Anesthesiology, Vanderbilt University Medical Center, Nashville, Tennessee, United States of America

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• M. Kay Washington,

  Roles Investigation

  Affiliation Department of Pathology, Microbiology, and Immunology, Vanderbilt University Medical Center, Nashville, Tennessee, United States of America

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• D. Borden Lacy

  Roles Conceptualization, Funding acquisition, Project administration, Supervision, Writing – review & editing

  * E-mail: borden.lacy@vanderbilt.edu

  Affiliations Department of Biochemistry, Vanderbilt University, Nashville, Tennessee, United States of America, Department of Pathology, Microbiology, and Immunology, Vanderbilt University Medical Center, Nashville, Tennessee, United States of America, Department of Veterans Affairs, Tennessee Valley Healthcare System, Nashville, Tennessee, United States of America

  https://orcid.org/0000-0003-2273-8121

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