Protective RBD-dimer vaccines against SARS-CoV-2 and its variants produced in glycoengineered Pichia pastoris

Tongxin Zhao; Sheng Liu; Pengyan Wang; Yanfang Zhang; Xinrui Kang; Xiaoqian Pan; Linjie Li; Dedong Li; Ping Gao; Yaling An; Hao Song; Kefang Liu; Jianxun Qi; Xin Zhao; Lianpan Dai; Peipei Liu; Peiyi Wang; Guizhen Wu; Taicheng Zhu; Kun Xu; Yin Li; George F. Gao

doi:10.1371/journal.ppat.1012487

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Open Access

Peer-reviewed

Research Article

Protective RBD-dimer vaccines against SARS-CoV-2 and its variants produced in glycoengineered Pichia pastoris

Tongxin Zhao,

Roles Formal analysis, Investigation, Visualization, Writing – original draft, Writing – review & editing

Affiliation CAS Key Laboratory of Pathogen Microbiology and Immunology, Institute of Microbiology, Chinese Academy of Sciences (CAS), Beijing, China
⨯
Sheng Liu,

Roles Investigation, Methodology

Affiliation Cryo-EM Center, Southern University of Science and Technology, Shenzhen, China
⨯
Pengyan Wang,

Roles Investigation

Affiliation CAS Key Laboratory of Pathogen Microbiology and Immunology, Institute of Microbiology, Chinese Academy of Sciences (CAS), Beijing, China
⨯
Yanfang Zhang,

Roles Investigation

Affiliation CAS Key Laboratory of Pathogen Microbiology and Immunology, Institute of Microbiology, Chinese Academy of Sciences (CAS), Beijing, China
⨯
Xinrui Kang,

Roles Investigation

Affiliation Medical School, University of Chinese Academy of Sciences (UCAS), Beijing, China
⨯
Xiaoqian Pan,

Roles Investigation

Affiliation Medical School, University of Chinese Academy of Sciences (UCAS), Beijing, China
⨯
Linjie Li,

Roles Investigation

Affiliation CAS Key Laboratory of Pathogen Microbiology and Immunology, Institute of Microbiology, Chinese Academy of Sciences (CAS), Beijing, China
⨯
Dedong Li,

Roles Investigation

Affiliation CAS Key Laboratory of Pathogen Microbiology and Immunology, Institute of Microbiology, Chinese Academy of Sciences (CAS), Beijing, China
⨯
Ping Gao,

Roles Investigation

Affiliation CAS Key Laboratory of Pathogen Microbiology and Immunology, Institute of Microbiology, Chinese Academy of Sciences (CAS), Beijing, China
⨯
Yaling An,

Roles Investigation

Affiliation CAS Key Laboratory of Pathogen Microbiology and Immunology, Institute of Microbiology, Chinese Academy of Sciences (CAS), Beijing, China
⨯
Hao Song,

Roles Formal analysis, Funding acquisition, Investigation, Writing – review & editing

Affiliation Research Network of Immunity and Health (RNIH), Beijing Institutes of Life Science, Chinese Academy of Sciences (CAS), Beijing, China
⨯
Kefang Liu,

Roles Investigation

Affiliation CAS Key Laboratory of Pathogen Microbiology and Immunology, Institute of Microbiology, Chinese Academy of Sciences (CAS), Beijing, China
⨯
Jianxun Qi,

Roles Formal analysis

Affiliation CAS Key Laboratory of Pathogen Microbiology and Immunology, Institute of Microbiology, Chinese Academy of Sciences (CAS), Beijing, China
⨯
Xin Zhao,

Roles Formal analysis, Methodology, Resources

Affiliation CAS Key Laboratory of Pathogen Microbiology and Immunology, Institute of Microbiology, Chinese Academy of Sciences (CAS), Beijing, China
⨯
Lianpan Dai,

Roles Conceptualization, Formal analysis, Funding acquisition

Affiliation CAS Key Laboratory of Pathogen Microbiology and Immunology, Institute of Microbiology, Chinese Academy of Sciences (CAS), Beijing, China
⨯
Peipei Liu ,

Roles Investigation, Resources

* E-mail: liupp@ivdc.chinacdc.cn (PL); zhutc@im.ac.cn (TZ); xukun@biols.ac.cn (KX)

Affiliation NHC Key Laboratory of Biosafety, National Institute for Viral Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing, China
⨯
Peiyi Wang,

Roles Resources

Affiliation Cryo-EM Center, Southern University of Science and Technology, Shenzhen, China
⨯
Guizhen Wu,

Roles Project administration, Resources

Affiliation NHC Key Laboratory of Biosafety, National Institute for Viral Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing, China
⨯
Taicheng Zhu ,

Roles Conceptualization, Formal analysis, Project administration, Writing – review & editing

* E-mail: liupp@ivdc.chinacdc.cn (PL); zhutc@im.ac.cn (TZ); xukun@biols.ac.cn (KX)

Affiliation Department of Microbial Physiological & Metabolic Engineering, State Key Laboratory of Microbial Resources, Institute of Microbiology, Chinese Academy of Sciences (CAS), Beijing, China
⨯
Kun Xu ,

Roles Conceptualization, Formal analysis, Project administration, Writing – original draft, Writing – review & editing

* E-mail: liupp@ivdc.chinacdc.cn (PL); zhutc@im.ac.cn (TZ); xukun@biols.ac.cn (KX)

Affiliation Research Network of Immunity and Health (RNIH), Beijing Institutes of Life Science, Chinese Academy of Sciences (CAS), Beijing, China

https://orcid.org/0000-0002-9762-5001

⨯
Yin Li,

Roles Conceptualization, Formal analysis, Project administration, Supervision, Writing – review & editing

Affiliation Department of Microbial Physiological & Metabolic Engineering, State Key Laboratory of Microbial Resources, Institute of Microbiology, Chinese Academy of Sciences (CAS), Beijing, China
⨯
[ ... ],
George F. Gao

Roles Conceptualization, Formal analysis, Funding acquisition, Project administration, Supervision, Writing – review & editing

Affiliations CAS Key Laboratory of Pathogen Microbiology and Immunology, Institute of Microbiology, Chinese Academy of Sciences (CAS), Beijing, China, Research Network of Immunity and Health (RNIH), Beijing Institutes of Life Science, Chinese Academy of Sciences (CAS), Beijing, China, NHC Key Laboratory of Biosafety, National Institute for Viral Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing, China

https://orcid.org/0000-0002-3869-615X

⨯
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Protective RBD-dimer vaccines against SARS-CoV-2 and its variants produced in glycoengineered Pichia pastoris

Tongxin Zhao,
Sheng Liu,
Pengyan Wang,
Yanfang Zhang,
Xinrui Kang,
Xiaoqian Pan,
Linjie Li,
Dedong Li,
Ping Gao,
Yaling An

Published: August 30, 2024
https://doi.org/10.1371/journal.ppat.1012487

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