Genome-wide CRISPR screenings identified SMCHD1 as a host-restricting factor for AAV transduction

Chenlu Wang; Yu Liu; Jingfei Xiong; Kun Xie; Tianshu Wang; Yu Hu; Huancheng Fu; Baiquan Zhang; Xiaochao Huang; Hui Bao; Haoyang Cai; Biao Dong; Zhonghan Li

doi:10.1371/journal.ppat.1012344

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Open Access

Peer-reviewed

Research Article

Genome-wide CRISPR screenings identified SMCHD1 as a host-restricting factor for AAV transduction

Chenlu Wang ,

Contributed equally to this work with: Chenlu Wang, Yu Liu

Roles Data curation, Formal analysis, Investigation, Methodology, Validation, Visualization, Writing – original draft

Affiliation Center of Growth Metabolism and Aging, State Key Laboratory of Oral Disease, West China Hospital of Stomatology, Key Laboratory of Bio-Resource and Eco-Environment of Ministry of Education, Animal Disease Prevention and Food Safety Key Laboratory of Sichuan Province, College of Life Sciences, Sichuan University, Chengdu, China
⨯
Yu Liu ,

Contributed equally to this work with: Chenlu Wang, Yu Liu

Roles Data curation, Formal analysis, Methodology

Affiliation National Clinical Research Center for Geriatrics and State Key Laboratory of Biotherapy, West China Hospital, Sichuan University, Chengdu, China
⨯
Jingfei Xiong,

Roles Formal analysis, Methodology, Visualization

Affiliation Center of Growth Metabolism and Aging, State Key Laboratory of Oral Disease, West China Hospital of Stomatology, Key Laboratory of Bio-Resource and Eco-Environment of Ministry of Education, Animal Disease Prevention and Food Safety Key Laboratory of Sichuan Province, College of Life Sciences, Sichuan University, Chengdu, China
⨯
Kun Xie,

Roles Data curation, Validation

Affiliation Center of Growth Metabolism and Aging, State Key Laboratory of Oral Disease, West China Hospital of Stomatology, Key Laboratory of Bio-Resource and Eco-Environment of Ministry of Education, Animal Disease Prevention and Food Safety Key Laboratory of Sichuan Province, College of Life Sciences, Sichuan University, Chengdu, China
⨯
Tianshu Wang,

Roles Formal analysis, Methodology, Visualization

Affiliation Center of Growth Metabolism and Aging, State Key Laboratory of Oral Disease, West China Hospital of Stomatology, Key Laboratory of Bio-Resource and Eco-Environment of Ministry of Education, Animal Disease Prevention and Food Safety Key Laboratory of Sichuan Province, College of Life Sciences, Sichuan University, Chengdu, China
⨯
Yu Hu,

Roles Data curation, Formal analysis

Affiliation Center of Growth Metabolism and Aging, State Key Laboratory of Oral Disease, West China Hospital of Stomatology, Key Laboratory of Bio-Resource and Eco-Environment of Ministry of Education, Animal Disease Prevention and Food Safety Key Laboratory of Sichuan Province, College of Life Sciences, Sichuan University, Chengdu, China
⨯
Huancheng Fu,

Roles Formal analysis, Validation, Visualization

Affiliation Center of Growth Metabolism and Aging, State Key Laboratory of Oral Disease, West China Hospital of Stomatology, Key Laboratory of Bio-Resource and Eco-Environment of Ministry of Education, Animal Disease Prevention and Food Safety Key Laboratory of Sichuan Province, College of Life Sciences, Sichuan University, Chengdu, China
⨯
Baiquan Zhang,

Roles Data curation, Methodology

Affiliation Center of Growth Metabolism and Aging, State Key Laboratory of Oral Disease, West China Hospital of Stomatology, Key Laboratory of Bio-Resource and Eco-Environment of Ministry of Education, Animal Disease Prevention and Food Safety Key Laboratory of Sichuan Province, College of Life Sciences, Sichuan University, Chengdu, China
⨯
Xiaochao Huang,

Roles Data curation, Methodology

Affiliation Center of Growth Metabolism and Aging, State Key Laboratory of Oral Disease, West China Hospital of Stomatology, Key Laboratory of Bio-Resource and Eco-Environment of Ministry of Education, Animal Disease Prevention and Food Safety Key Laboratory of Sichuan Province, College of Life Sciences, Sichuan University, Chengdu, China
⨯
Hui Bao,

Roles Data curation, Methodology, Validation

Affiliation Center of Growth Metabolism and Aging, State Key Laboratory of Oral Disease, West China Hospital of Stomatology, Key Laboratory of Bio-Resource and Eco-Environment of Ministry of Education, Animal Disease Prevention and Food Safety Key Laboratory of Sichuan Province, College of Life Sciences, Sichuan University, Chengdu, China
⨯
Haoyang Cai,

Roles Funding acquisition, Supervision, Writing – review & editing

Affiliation Center of Growth Metabolism and Aging, State Key Laboratory of Oral Disease, West China Hospital of Stomatology, Key Laboratory of Bio-Resource and Eco-Environment of Ministry of Education, Animal Disease Prevention and Food Safety Key Laboratory of Sichuan Province, College of Life Sciences, Sichuan University, Chengdu, China
⨯
Biao Dong ,

Roles Conceptualization, Supervision, Writing – review & editing

* E-mail: Biaodong@scu.edu.cn (BD); Zhonghan.Li@outlook.com (ZL)

Affiliations National Clinical Research Center for Geriatrics and State Key Laboratory of Biotherapy, West China Hospital, Sichuan University, Chengdu, China, Sichuan Real and Best Biotech Co., Ltd., Chengdu, China
⨯
Zhonghan Li

Roles Conceptualization, Funding acquisition, Project administration, Resources, Supervision, Writing – original draft, Writing – review & editing

* E-mail: Biaodong@scu.edu.cn (BD); Zhonghan.Li@outlook.com (ZL)

Affiliation Center of Growth Metabolism and Aging, State Key Laboratory of Oral Disease, West China Hospital of Stomatology, Key Laboratory of Bio-Resource and Eco-Environment of Ministry of Education, Animal Disease Prevention and Food Safety Key Laboratory of Sichuan Province, College of Life Sciences, Sichuan University, Chengdu, China

https://orcid.org/0000-0001-5752-3417

⨯

Genome-wide CRISPR screenings identified SMCHD1 as a host-restricting factor for AAV transduction

Chenlu Wang,
Yu Liu,
Jingfei Xiong,
Kun Xie,
Tianshu Wang,
Yu Hu,
Huancheng Fu,
Baiquan Zhang,
Xiaochao Huang,
Hui Bao

Published: July 8, 2024
https://doi.org/10.1371/journal.ppat.1012344

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