Demonstrating the importance of porcine reproductive and respiratory syndrome virus papain-like protease 2 deubiquitinating activity in viral replication by structure-guided mutagenesis

• Ben A. Bailey-Elkin ,

  Contributed equally to this work with: Ben A. Bailey-Elkin, Robert C. M. Knaap, Marjolein Kikkert, Brian L. Mark

  Roles Conceptualization, Investigation, Writing – original draft, Writing – review & editing

  Current address: One Health Division, Public Health Agency of Canada, National Microbiology Laboratory, Winnipeg, Manitoba Canada

  Affiliation Department of Microbiology, University of Manitoba, Winnipeg, Manitoba, Canada

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• Robert C. M. Knaap ,

  Contributed equally to this work with: Ben A. Bailey-Elkin, Robert C. M. Knaap, Marjolein Kikkert, Brian L. Mark

  Roles Conceptualization, Investigation, Writing – review & editing

  Affiliation Molecular Virology Laboratory, Department of Medical Microbiology, Leiden University Center of Infectious Diseases (LU-CID), Leiden University Medical Center, Leiden, The Netherlands

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• Anuradha De Silva,

  Roles Investigation

  Affiliation Department of Microbiology, University of Manitoba, Winnipeg, Manitoba, Canada

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• Ilse M. Boekhoud,

  Roles Investigation

  Affiliation Molecular Virology Laboratory, Department of Medical Microbiology, Leiden University Center of Infectious Diseases (LU-CID), Leiden University Medical Center, Leiden, The Netherlands

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• Sandra Mous,

  Roles Investigation

  Current address: Linac Coherent Light Source, SLAC National Accelerator Laboratory, Menlo Park, United States of America

  Affiliation Molecular Virology Laboratory, Department of Medical Microbiology, Leiden University Center of Infectious Diseases (LU-CID), Leiden University Medical Center, Leiden, The Netherlands

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• Niek van Vught,

  Roles Investigation

  Affiliation Molecular Virology Laboratory, Department of Medical Microbiology, Leiden University Center of Infectious Diseases (LU-CID), Leiden University Medical Center, Leiden, The Netherlands

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• Mazdak Khajehpour,

  Roles Formal analysis, Methodology, Validation, Writing – review & editing

  Affiliation Department of Chemistry, University of Manitoba, Winnipeg, Manitoba, Canada

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• Erwin van den Born,

  Roles Conceptualization, Writing – review & editing

  Affiliation MSD Animal Health, Boxmeer, The Netherlands

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• Marjolein Kikkert ,

  Contributed equally to this work with: Ben A. Bailey-Elkin, Robert C. M. Knaap, Marjolein Kikkert, Brian L. Mark

  Roles Conceptualization, Formal analysis, Funding acquisition, Investigation, Methodology, Project administration, Resources, Supervision, Validation, Visualization, Writing – original draft, Writing – review & editing

  * E-mail: m.kikkert@lumc.nl (MK); brian.mark@umanitoba.ca (BLM)

  Affiliation Molecular Virology Laboratory, Department of Medical Microbiology, Leiden University Center of Infectious Diseases (LU-CID), Leiden University Medical Center, Leiden, The Netherlands

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• Brian L. Mark

  Contributed equally to this work with: Ben A. Bailey-Elkin, Robert C. M. Knaap, Marjolein Kikkert, Brian L. Mark

  Roles Conceptualization, Formal analysis, Funding acquisition, Investigation, Methodology, Project administration, Resources, Supervision, Validation, Writing – original draft, Writing – review & editing

  * E-mail: m.kikkert@lumc.nl (MK); brian.mark@umanitoba.ca (BLM)

  Affiliation Department of Microbiology, University of Manitoba, Winnipeg, Manitoba, Canada

  https://orcid.org/0000-0002-7344-1355

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