Development of a bispecific nanobody conjugate broadly neutralizes diverse SARS-CoV-2 variants and structural basis for its broad neutralization

Jing Yang; Sheng Lin; Zimin Chen; Fanli Yang; Liyan Guo; Lingling Wang; Yanping Duan; Xindan Zhang; Yushan Dai; Keqing Yin; Chongzhang Yu; Xin Yuan; Honglu Sun; Bin He; Yu Cao; Haoyu Ye; Haohao Dong; Xianbo Liu; Bo Chen; Jian Li; Qi Zhao; Guangwen Lu

doi:10.1371/journal.ppat.1011804

Loading metrics

Open Access

Peer-reviewed

Research Article

Development of a bispecific nanobody conjugate broadly neutralizes diverse SARS-CoV-2 variants and structural basis for its broad neutralization

Jing Yang ,

Contributed equally to this work with: Jing Yang, Sheng Lin

Roles Formal analysis, Investigation, Validation, Visualization, Writing – original draft, Writing – review & editing

Affiliation Department of Emergency Medicine, State Key Laboratory of Biotherapy, West China Hospital, Sichuan University, Chengdu, Sichuan, China
⨯
Sheng Lin ,

Contributed equally to this work with: Jing Yang, Sheng Lin

Roles Formal analysis, Funding acquisition, Investigation, Validation, Visualization, Writing – original draft, Writing – review & editing

Affiliation Department of Emergency Medicine, State Key Laboratory of Biotherapy, West China Hospital, Sichuan University, Chengdu, Sichuan, China
⨯
Zimin Chen,

Roles Formal analysis, Funding acquisition, Investigation, Validation

Affiliation Department of Emergency Medicine, State Key Laboratory of Biotherapy, West China Hospital, Sichuan University, Chengdu, Sichuan, China
⨯
Fanli Yang,

Roles Formal analysis, Funding acquisition, Validation, Visualization

Affiliation Department of Emergency Medicine, State Key Laboratory of Biotherapy, West China Hospital, Sichuan University, Chengdu, Sichuan, China
⨯
Liyan Guo,

Roles Formal analysis, Investigation, Visualization

Affiliation Department of Emergency Medicine, State Key Laboratory of Biotherapy, West China Hospital, Sichuan University, Chengdu, Sichuan, China
⨯
Lingling Wang,

Roles Investigation

Affiliation Department of Emergency Medicine, State Key Laboratory of Biotherapy, West China Hospital, Sichuan University, Chengdu, Sichuan, China
⨯
Yanping Duan,

Roles Investigation

Affiliation Department of Emergency Medicine, State Key Laboratory of Biotherapy, West China Hospital, Sichuan University, Chengdu, Sichuan, China
⨯
Xindan Zhang,

Roles Investigation

Affiliation Department of Emergency Medicine, State Key Laboratory of Biotherapy, West China Hospital, Sichuan University, Chengdu, Sichuan, China
⨯
Yushan Dai,

Roles Investigation

Affiliation Department of Emergency Medicine, State Key Laboratory of Biotherapy, West China Hospital, Sichuan University, Chengdu, Sichuan, China
⨯
Keqing Yin,

Roles Investigation

Affiliation Department of Emergency Medicine, State Key Laboratory of Biotherapy, West China Hospital, Sichuan University, Chengdu, Sichuan, China
⨯
Chongzhang Yu,

Roles Investigation

Affiliation Department of Emergency Medicine, State Key Laboratory of Biotherapy, West China Hospital, Sichuan University, Chengdu, Sichuan, China
⨯
Xin Yuan,

Roles Investigation

Affiliation Department of Emergency Medicine, State Key Laboratory of Biotherapy, West China Hospital, Sichuan University, Chengdu, Sichuan, China
⨯
Honglu Sun,

Roles Investigation

Affiliation Department of Emergency Medicine, State Key Laboratory of Biotherapy, West China Hospital, Sichuan University, Chengdu, Sichuan, China
⨯
Bin He,

Roles Resources, Writing – review & editing

Affiliation Department of Emergency Medicine, State Key Laboratory of Biotherapy, West China Hospital, Sichuan University, Chengdu, Sichuan, China
⨯
Yu Cao,

Roles Resources, Writing – review & editing

Affiliations Department of Emergency Medicine, State Key Laboratory of Biotherapy, West China Hospital, Sichuan University, Chengdu, Sichuan, China, Disaster Medicine Center, West China Hospital, Sichuan University, Chengdu, Sichuan, China
⨯
Haoyu Ye,

Roles Resources, Writing – review & editing

Affiliation Department of Biotherapy, State Key Laboratory of Biotherapy and Cancer Center, West China Hospital, Sichuan University, Chengdu, Sichuan, China
⨯
Haohao Dong,

Roles Methodology, Writing – review & editing

Affiliation State Key Laboratory of Biotherapy and Cancer Center, National Clinical Research Center for Geriatrics, West China Hospital, Sichuan University, Chengdu, Sichuan, China
⨯
Xianbo Liu,

Roles Investigation

Affiliation CHENGDU NB BIOLAB CO., LTD, Chengdu, Sichuan, China
⨯
Bo Chen,

Roles Investigation

Affiliation CHENGDU NB BIOLAB CO., LTD, Chengdu, Sichuan, China
⨯
Jian Li,

Roles Methodology, Writing – review & editing

Affiliation School of Basic Medical Sciences, Chengdu University, Chengdu, Sichuan, China
⨯
Qi Zhao,

Roles Methodology, Writing – review & editing

Affiliation College of Food and Biological Engineering, Chengdu University, Chengdu, Sichuan, China
⨯
[ ... ],
Guangwen Lu

Roles Conceptualization, Formal analysis, Funding acquisition, Methodology, Resources, Supervision, Writing – original draft, Writing – review & editing

* E-mail: lugw@scu.edu.cn

Affiliation Department of Emergency Medicine, State Key Laboratory of Biotherapy, West China Hospital, Sichuan University, Chengdu, Sichuan, China

https://orcid.org/0000-0001-7568-592X

⨯
[ view all ]
[ view less ]

Development of a bispecific nanobody conjugate broadly neutralizes diverse SARS-CoV-2 variants and structural basis for its broad neutralization

Jing Yang,
Sheng Lin,
Zimin Chen,
Fanli Yang,
Liyan Guo,
Lingling Wang,
Yanping Duan,
Xindan Zhang,
Yushan Dai,
Keqing Yin

Published: November 30, 2023
https://doi.org/10.1371/journal.ppat.1011804

Reader Comments (0)

Post a new comment on this article

Subject Areas
?

For more information about PLOS Subject Areas, click here.
We want your feedback. Do these Subject Areas make sense for this article? Click the target next to the incorrect Subject Area and let us know. Thanks for your help!

SARS CoV 2
Is the Subject Area "SARS CoV 2" applicable to this article?

Thanks for your feedback.
Antibodies
Is the Subject Area "Antibodies" applicable to this article?

Thanks for your feedback.
Binding analysis
Is the Subject Area "Binding analysis" applicable to this article?

Thanks for your feedback.
Enzyme-linked immunoassays
Is the Subject Area "Enzyme-linked immunoassays" applicable to this article?

Thanks for your feedback.
SARS coronavirus
Is the Subject Area "SARS coronavirus" applicable to this article?

Thanks for your feedback.
Crystal structure
Is the Subject Area "Crystal structure" applicable to this article?

Thanks for your feedback.
Health care
Is the Subject Area "Health care" applicable to this article?

Thanks for your feedback.
Bionanotechnology
Is the Subject Area "Bionanotechnology" applicable to this article?

Thanks for your feedback.