A sporulation signature protease is required for assembly of the spore surface layers, germination and host colonization in Clostridioides difficile

• Eleonora Marini,

  Roles Formal analysis, Investigation

  Affiliation Instituto de Tecnologia Química e Biológica António Xavier, Universidade Nova de Lisboa, Avenida da República EAN, Oeiras, Portugal

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• Carmen Olivença,

  Roles Formal analysis, Investigation

  Affiliation Instituto de Tecnologia Química e Biológica António Xavier, Universidade Nova de Lisboa, Avenida da República EAN, Oeiras, Portugal

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• Sara Ramalhete,

  Roles Investigation

  Affiliation Instituto de Tecnologia Química e Biológica António Xavier, Universidade Nova de Lisboa, Avenida da República EAN, Oeiras, Portugal

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• Andrea Martinez Aguirre,

  Roles Investigation

  Affiliation Texas A&M University, Department of Biology, College Station, Texas, United States of America

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• Patrick Ingle,

  Roles Investigation

  Affiliation Clostridia Research Group, BBSRC/EPSRC Synthetic Biology Research Centre (SBRC), School of Life Sciences, University of Nottingham, Nottingham, United Kingdom

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• Manuel N. Melo,

  Roles Formal analysis, Investigation

  Affiliation Instituto de Tecnologia Química e Biológica António Xavier, Universidade Nova de Lisboa, Avenida da República EAN, Oeiras, Portugal

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• Wilson Antunes,

  Roles Investigation

  Affiliation Instituto de Tecnologia Química e Biológica António Xavier, Universidade Nova de Lisboa, Avenida da República EAN, Oeiras, Portugal

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• Nigel P. Minton,

  Roles Supervision

  Affiliation Clostridia Research Group, BBSRC/EPSRC Synthetic Biology Research Centre (SBRC), School of Life Sciences, University of Nottingham, Nottingham, United Kingdom

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• Guillem Hernandez,

  Roles Investigation

  Affiliation Instituto de Tecnologia Química e Biológica António Xavier, Universidade Nova de Lisboa, Avenida da República EAN, Oeiras, Portugal

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• Tiago N. Cordeiro,

  Roles Supervision

  Affiliation Instituto de Tecnologia Química e Biológica António Xavier, Universidade Nova de Lisboa, Avenida da República EAN, Oeiras, Portugal

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• Joseph A. Sorg,

  Roles Formal analysis, Funding acquisition, Supervision, Writing – review & editing

  Affiliation Texas A&M University, Department of Biology, College Station, Texas, United States of America

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• Mónica Serrano,

  Roles Funding acquisition, Supervision, Writing – review & editing

  Affiliation Instituto de Tecnologia Química e Biológica António Xavier, Universidade Nova de Lisboa, Avenida da República EAN, Oeiras, Portugal

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• Adriano O. Henriques

  Roles Conceptualization, Formal analysis, Funding acquisition, Project administration, Resources, Supervision, Writing – original draft, Writing – review & editing

  * E-mail: aoh@itqb.unl.pt

  Affiliation Instituto de Tecnologia Química e Biológica António Xavier, Universidade Nova de Lisboa, Avenida da República EAN, Oeiras, Portugal

  https://orcid.org/0000-0003-4292-4802

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