Spike substitution T813S increases Sarbecovirus fusogenicity by enhancing the usage of TMPRSS2

Yong Ma; Pengbin Li; Yunqi Hu; Tianyi Qiu; Lixiang Wang; Hongjie Lu; Kexin Lv; Mengxin Xu; Jiaxin Zhuang; Xue Liu; Suhua He; Bing He; Shuning Liu; Lin Liu; Yuanyuan Wang; Xinyu Yue; Yanmei Zhai; Wanyu Luo; Haoting Mai; Yu Kuang; Shifeng Chen; Feng Ye; Na Zhou; Wenjing Zhao; Jun Chen; Shoudeng Chen; Xiaoli Xiong; Mang Shi; Ji-An Pan; Yao-Qing Chen

doi:10.1371/journal.ppat.1011123

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Open Access

Peer-reviewed

Research Article

Spike substitution T813S increases Sarbecovirus fusogenicity by enhancing the usage of TMPRSS2

Yong Ma ,

Contributed equally to this work with: Yong Ma, Pengbin Li, Yunqi Hu

Roles Conceptualization, Data curation, Formal analysis, Investigation, Methodology, Validation, Visualization, Writing – original draft, Writing – review & editing

Affiliation School of Public Health (Shenzhen), Shenzhen Campus of Sun Yat-sen University, Shenzhen, China
⨯
Pengbin Li ,

Contributed equally to this work with: Yong Ma, Pengbin Li, Yunqi Hu

Roles Data curation, Formal analysis, Investigation, Methodology, Validation, Writing – original draft

Affiliation School of Public Health (Shenzhen), Shenzhen Campus of Sun Yat-sen University, Shenzhen, China
⨯
Yunqi Hu ,

Contributed equally to this work with: Yong Ma, Pengbin Li, Yunqi Hu

Roles Formal analysis, Investigation, Methodology, Validation, Writing – original draft

Affiliation School of Public Health (Shenzhen), Shenzhen Campus of Sun Yat-sen University, Shenzhen, China
⨯
Tianyi Qiu,

Roles Conceptualization, Investigation, Methodology, Software, Visualization

Affiliation Institute of Clinical Science, Zhongshan Hospital, Shanghai Medical College, Fudan University, Shanghai, China
⨯
Lixiang Wang,

Roles Visualization

Affiliation Department of Immunology and Microbiology, Zhongshan School of Medicine, Sun Yat-sen University, Guangzhou, China
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Hongjie Lu,

Roles Resources

Affiliation School of Public Health (Shenzhen), Shenzhen Campus of Sun Yat-sen University, Shenzhen, China
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Kexin Lv,

Roles Resources

Affiliation School of Public Health (Shenzhen), Shenzhen Campus of Sun Yat-sen University, Shenzhen, China
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Mengxin Xu,

Roles Resources

Affiliation School of Public Health (Shenzhen), Shenzhen Campus of Sun Yat-sen University, Shenzhen, China
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Jiaxin Zhuang,

Roles Methodology, Resources

Affiliation The Center for Infection and Immunity Study and Molecular Cancer Research Center, School of Medicine, Shenzhen Campus of Sun Yat-sen University, Shenzhen, China
⨯
Xue Liu,

Roles Resources, Visualization

Affiliation The Center for Infection and Immunity Study and Molecular Cancer Research Center, School of Medicine, Shenzhen Campus of Sun Yat-sen University, Shenzhen, China
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Suhua He,

Roles Resources

Affiliation Molecular Imaging Center, Guangdong Provincial Key Laboratory of Biomedical Imaging, the Fifth Affiliated Hospital, Sun Yat-sen University, Zhuhai, China
⨯
Bing He,

Roles Resources

Affiliation School of Public Health (Shenzhen), Shenzhen Campus of Sun Yat-sen University, Shenzhen, China
⨯
Shuning Liu,

Roles Resources

Affiliation School of Public Health (Shenzhen), Shenzhen Campus of Sun Yat-sen University, Shenzhen, China
⨯
Lin Liu,

Roles Resources

Affiliation School of Public Health (Shenzhen), Shenzhen Campus of Sun Yat-sen University, Shenzhen, China
⨯
Yuanyuan Wang,

Roles Resources

Affiliation School of Public Health (Shenzhen), Shenzhen Campus of Sun Yat-sen University, Shenzhen, China
⨯
Xinyu Yue,

Roles Resources

Affiliation School of Public Health (Shenzhen), Shenzhen Campus of Sun Yat-sen University, Shenzhen, China
⨯
Yanmei Zhai,

Roles Resources

Affiliation School of Public Health (Shenzhen), Shenzhen Campus of Sun Yat-sen University, Shenzhen, China
⨯
Wanyu Luo,

Roles Resources

Affiliation School of Public Health (Shenzhen), Shenzhen Campus of Sun Yat-sen University, Shenzhen, China
⨯
Haoting Mai,

Roles Resources

Affiliation School of Public Health (Shenzhen), Shenzhen Campus of Sun Yat-sen University, Shenzhen, China
⨯
Yu Kuang,

Roles Resources

Affiliation School of Public Health (Shenzhen), Shenzhen Campus of Sun Yat-sen University, Shenzhen, China
⨯
Shifeng Chen,

Roles Resources

Affiliation The 74(th) Group Army Hospital, Guangzhou, China
⨯
Feng Ye,

Roles Resources

Affiliation The 74(th) Group Army Hospital, Guangzhou, China
⨯
Na Zhou,

Roles Resources

Affiliation The 74(th) Group Army Hospital, Guangzhou, China
⨯
Wenjing Zhao,

Roles Investigation

Affiliation The Center for Infection and Immunity Study and Molecular Cancer Research Center, School of Medicine, Shenzhen Campus of Sun Yat-sen University, Shenzhen, China
⨯
Jun Chen,

Roles Investigation, Resources

Affiliation Department of Immunology and Microbiology, Zhongshan School of Medicine, Sun Yat-sen University, Guangzhou, China
⨯
Shoudeng Chen,

Roles Resources

Affiliation Molecular Imaging Center, Guangdong Provincial Key Laboratory of Biomedical Imaging, the Fifth Affiliated Hospital, Sun Yat-sen University, Zhuhai, China
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Xiaoli Xiong,

Roles Investigation, Writing – review & editing

Affiliation State Key Laboratory of Respiratory Disease, CAS Key Laboratory of Regenerative Biology, Guangdong Provincial Key Laboratory of Stem Cell and Regenerative Medicine, Guangzhou Institutes of Biomedicine and Health, Chinese Academy of Sciences, Guangzhou, China
⨯
Mang Shi,

Roles Investigation, Methodology, Software, Visualization, Writing – review & editing

Affiliation The Center for Infection and Immunity Study and Molecular Cancer Research Center, School of Medicine, Shenzhen Campus of Sun Yat-sen University, Shenzhen, China
⨯
Ji-An Pan ,

Roles Investigation, Methodology

* E-mail: panjan@mail.sysu.edu.cn (JAP); chenyaoqing@mail.sysu.edu.cn (YQC)

Affiliation The Center for Infection and Immunity Study and Molecular Cancer Research Center, School of Medicine, Shenzhen Campus of Sun Yat-sen University, Shenzhen, China
⨯
[ ... ],
Yao-Qing Chen

Roles Conceptualization, Funding acquisition, Investigation, Project administration, Supervision, Writing – review & editing

* E-mail: panjan@mail.sysu.edu.cn (JAP); chenyaoqing@mail.sysu.edu.cn (YQC)

Affiliations School of Public Health (Shenzhen), Shenzhen Campus of Sun Yat-sen University, Shenzhen, China, National Medical Products Administration Key Laboratory for Quality Monitoring and Evaluation of Vaccines and Biological Products, Sun Yat-sen University, Guanzhou, China

https://orcid.org/0000-0002-4659-1550

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Spike substitution T813S increases Sarbecovirus fusogenicity by enhancing the usage of TMPRSS2

Yong Ma,
Pengbin Li,
Yunqi Hu,
Tianyi Qiu,
Lixiang Wang,
Hongjie Lu,
Kexin Lv,
Mengxin Xu,
Jiaxin Zhuang,
Xue Liu

Published: May 17, 2023
https://doi.org/10.1371/journal.ppat.1011123

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