High-throughput saturation mutagenesis generates a high-affinity antibody against SARS-CoV-2 variants using protein surface display assay on a human cell

• Ye Yang ,

  Contributed equally to this work with: Ye Yang, Shuo Liu

  Roles Data curation, Formal analysis, Investigation, Methodology, Validation, Visualization, Writing – original draft, Writing – review & editing

  Affiliation State Key Laboratory of Natural and Biomimetic Drugs, Department of Biomedical Engineering, College of Future Technology, Peking University, Beijing, China

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• Shuo Liu ,

  Contributed equally to this work with: Ye Yang, Shuo Liu

  Roles Data curation, Investigation, Validation

  Affiliations Graduate School of Chinese Academy of Medical Sciences, Peking Union Medical College, Beijing, China, Division of HIV/AIDS and Sex-transmitted Virus Vaccines, Institute for Biological Product Control, National Institutes for Food and Drug Control (NIFDC), Beijing, China

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• Yufeng Luo,

  Roles Data curation

  Affiliation State Key Laboratory of Natural and Biomimetic Drugs, Department of Biomedical Engineering, College of Future Technology, Peking University, Beijing, China

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• Bolun Wang,

  Roles Data curation

  Affiliation State Key Laboratory of Natural and Biomimetic Drugs, Department of Biomedical Engineering, College of Future Technology, Peking University, Beijing, China

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• Junyi Wang,

  Roles Data curation, Software

  Affiliation State Key Laboratory of Natural and Biomimetic Drugs, Department of Biomedical Engineering, College of Future Technology, Peking University, Beijing, China

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• Juan Li,

  Roles Data curation

  Affiliation State Key Laboratory of Natural and Biomimetic Drugs, Department of Biomedical Engineering, College of Future Technology, Peking University, Beijing, China

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• Jiaxin Li,

  Roles Data curation

  Affiliation State Key Laboratory of Natural and Biomimetic Drugs, Department of Biomedical Engineering, College of Future Technology, Peking University, Beijing, China

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• Buqing Ye,

  Roles Project administration

  Affiliation State Key Laboratory of Natural and Biomimetic Drugs, Department of Biomedical Engineering, College of Future Technology, Peking University, Beijing, China

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• Youchun Wang ,

  Roles Funding acquisition, Resources, Supervision

  * E-mail: wangyc@nifdc.org.cn (YW); jzxi@pku.edu.cn (JJX)

  Affiliation Division of HIV/AIDS and Sex-transmitted Virus Vaccines, Institute for Biological Product Control, National Institutes for Food and Drug Control (NIFDC), Beijing, China

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• Jianzhong Jeff Xi

  Roles Conceptualization, Funding acquisition, Project administration, Resources, Supervision, Visualization, Writing – original draft, Writing – review & editing

  * E-mail: wangyc@nifdc.org.cn (YW); jzxi@pku.edu.cn (JJX)

  Affiliation State Key Laboratory of Natural and Biomimetic Drugs, Department of Biomedical Engineering, College of Future Technology, Peking University, Beijing, China

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