A modular CRISPR screen identifies individual and combination pathways contributing to HIV-1 latency

Emily Hsieh; Derek H. Janssens; Patrick J. Paddison; Edward P. Browne; Steve Henikoff; Molly OhAinle; Michael Emerman

doi:10.1371/journal.ppat.1011101

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Open Access

Peer-reviewed

Research Article

A modular CRISPR screen identifies individual and combination pathways contributing to HIV-1 latency

Emily Hsieh,

Roles Conceptualization, Data curation, Formal analysis, Investigation, Methodology, Validation, Visualization, Writing – original draft, Writing – review & editing

Affiliation Molecular and Cellular Biology Graduate Program, University of Washington, Seattle, Washington, United States of America
⨯
Derek H. Janssens,

Roles Investigation, Methodology, Writing – original draft, Writing – review & editing

Affiliation Division of Basic Sciences, Fred Hutchinson Cancer Center, Seattle, Washington, United States of America
⨯
Patrick J. Paddison,

Roles Methodology, Resources

Affiliation Division of Human Biology, Fred Hutchinson Cancer Center, Seattle, Washington, United States of America
⨯
Edward P. Browne,

Roles Methodology, Resources

Affiliation Division of Infectious Diseases, Department of Medicine, University of North Carolina at Chapel Hill, Chapel Hill, North Carolina, United States of America
⨯
Steve Henikoff,

Roles Funding acquisition, Methodology, Supervision, Writing – review & editing

Affiliations Division of Basic Sciences, Fred Hutchinson Cancer Center, Seattle, Washington, United States of America, Howard Hughes Medical Institute, Chevy Chase, Maryland, United States of America
⨯
Molly OhAinle,

Roles Conceptualization, Methodology, Supervision, Writing – review & editing

Current Address: Division of Immunology and Molecular Medicine, Department of Molecular and Cell Biology, University of California, Berkeley, California, United States of America

Affiliation Division of Human Biology, Fred Hutchinson Cancer Center, Seattle, Washington, United States of America
⨯
Michael Emerman

Roles Conceptualization, Funding acquisition, Investigation, Project administration, Supervision, Writing – original draft, Writing – review & editing

* E-mail: memerman@fredhutch.org

Affiliations Division of Basic Sciences, Fred Hutchinson Cancer Center, Seattle, Washington, United States of America, Division of Human Biology, Fred Hutchinson Cancer Center, Seattle, Washington, United States of America

https://orcid.org/0000-0002-4181-6335

⨯

A modular CRISPR screen identifies individual and combination pathways contributing to HIV-1 latency

Emily Hsieh,
Derek H. Janssens,
Patrick J. Paddison,
Edward P. Browne,
Steve Henikoff,
Molly OhAinle,
Michael Emerman

Published: January 27, 2023
https://doi.org/10.1371/journal.ppat.1011101

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HIV-1
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Viral persistence and latency
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Viral replication
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Genetic screens
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Genomics
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T helper cells
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