CRISPR/FnCas12a-mediated efficient multiplex and iterative genome editing in bacterial plant pathogens without donor DNA templates

Fang Yan; Jingwen Wang; Sujie Zhang; Zhenwan Lu; Shaofang Li; Zhiyuan Ji; Congfeng Song; Gongyou Chen; Jin Xu; Jie Feng; Xueping Zhou; Huanbin Zhou

doi:10.1371/journal.ppat.1010961

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Open Access

Peer-reviewed

Research Article

CRISPR/FnCas12a-mediated efficient multiplex and iterative genome editing in bacterial plant pathogens without donor DNA templates

Fang Yan ,

Contributed equally to this work with: Fang Yan, Jingwen Wang, Sujie Zhang

Roles Investigation, Writing – original draft

Affiliation State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing, China
⨯
Jingwen Wang ,

Contributed equally to this work with: Fang Yan, Jingwen Wang, Sujie Zhang

Roles Investigation

Current address: Zhoukou Academy of Agricultural Sciences, Zhoukou, Henan, China

Affiliation State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing, China
⨯
Sujie Zhang ,

Contributed equally to this work with: Fang Yan, Jingwen Wang, Sujie Zhang

Roles Investigation, Writing – original draft

Affiliations State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing, China, Scientific Observing and Experimental Station of Crop Pests in Guilin, Ministry of Agriculture and Rural Affairs, Guilin, China
⨯
Zhenwan Lu,

Roles Investigation

Affiliations State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing, China, Scientific Observing and Experimental Station of Crop Pests in Guilin, Ministry of Agriculture and Rural Affairs, Guilin, China
⨯
Shaofang Li,

Roles Investigation

Affiliation State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing, China
⨯
Zhiyuan Ji,

Roles Resources

Affiliation National Key Facility for Crop Gene Resources and Genetic Improvement, Institute of Crop Sciences, Chinese Academy of Agricultural Sciences, Beijing, China
⨯
Congfeng Song,

Roles Conceptualization, Resources

Affiliation Key Laboratory of Monitoring and Management of Plant Diseases and Insects, Ministry of Education, Nanjing Agricultural University, Nanjing, China
⨯
Gongyou Chen,

Roles Conceptualization, Resources

Affiliation State Key Laboratory of Microbial Metabolism, School of Agriculture and Biology, Shanghai Jiao Tong University, Shanghai, China
⨯
Jin Xu,

Roles Resources

Affiliation State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing, China
⨯
Jie Feng,

Roles Conceptualization

Affiliation State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing, China
⨯
Xueping Zhou,

Roles Conceptualization

Affiliations State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing, China, State Key Laboratory of Rice Biology, Institute of Biotechnology, Zhejiang University, Hangzhou, China
⨯
Huanbin Zhou

Roles Conceptualization, Supervision, Writing – original draft, Writing – review & editing

* E-mail: zhouhuanbin@caas.cn

Affiliations State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing, China, Scientific Observing and Experimental Station of Crop Pests in Guilin, Ministry of Agriculture and Rural Affairs, Guilin, China

https://orcid.org/0000-0002-9471-7867

⨯

CRISPR/FnCas12a-mediated efficient multiplex and iterative genome editing in bacterial plant pathogens without donor DNA templates

Fang Yan,
Jingwen Wang,
Sujie Zhang,
Zhenwan Lu,
Shaofang Li,
Zhiyuan Ji,
Congfeng Song,
Gongyou Chen,
Jin Xu,
Jie Feng

Published: January 10, 2023
https://doi.org/10.1371/journal.ppat.1010961

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CRISPR
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Bacterial genomics
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Genomics
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Plant bacterial pathogens
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Polymerase chain reaction
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DNA repair
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Non-homologous end joining
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