Genome wide CRISPR screen for Pasteurella multocida toxin (PMT) binding proteins reveals LDL Receptor Related Protein 1 (LRP1) as crucial cellular receptor

Julian Schoellkopf; Thomas Mueller; Lena Hippchen; Teresa Mueller; Raphael Reuten; Rolf Backofen; Joachim Orth; Gudula Schmidt

doi:10.1371/journal.ppat.1010781

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Open Access

Peer-reviewed

Research Article

Genome wide CRISPR screen for Pasteurella multocida toxin (PMT) binding proteins reveals LDL Receptor Related Protein 1 (LRP1) as crucial cellular receptor

Julian Schoellkopf,

Roles Conceptualization, Data curation, Investigation

Affiliation Institute for Experimental and Clinical Pharmacology and Toxicology, Faculty of Medicine, Albert-Ludwigs-University, Freiburg, Germany
⨯
Thomas Mueller,

Roles Conceptualization, Data curation, Investigation

Affiliation Institute for Experimental and Clinical Pharmacology and Toxicology, Faculty of Medicine, Albert-Ludwigs-University, Freiburg, Germany
⨯
Lena Hippchen,

Roles Data curation, Investigation

Affiliation Institute for Experimental and Clinical Pharmacology and Toxicology, Faculty of Medicine, Albert-Ludwigs-University, Freiburg, Germany
⨯
Teresa Mueller,

Roles Data curation, Formal analysis, Validation

Affiliation Bioinformatics—Department of Computer Science, Albert-Ludwigs-University, Freiburg, Germany
⨯
Raphael Reuten,

Roles Data curation

Affiliation Institute for Experimental and Clinical Pharmacology and Toxicology, Faculty of Medicine, Albert-Ludwigs-University, Freiburg, Germany
⨯
Rolf Backofen,

Roles Funding acquisition

Affiliations Bioinformatics—Department of Computer Science, Albert-Ludwigs-University, Freiburg, Germany, Signalling Research Centres BIOSS and CIBSS, Albert-Ludwigs-University, Freiburg, Germany
⨯
Joachim Orth,

Roles Project administration

Affiliation Institute for Experimental and Clinical Pharmacology and Toxicology, Faculty of Medicine, Albert-Ludwigs-University, Freiburg, Germany
⨯
Gudula Schmidt

Roles Conceptualization, Data curation, Funding acquisition, Writing – original draft, Writing – review & editing

* E-mail: Gudula.Schmidt@pharmakol.uni-freiburg.de

Affiliation Institute for Experimental and Clinical Pharmacology and Toxicology, Faculty of Medicine, Albert-Ludwigs-University, Freiburg, Germany

https://orcid.org/0000-0002-2876-0189

⨯

Genome wide CRISPR screen for Pasteurella multocida toxin (PMT) binding proteins reveals LDL Receptor Related Protein 1 (LRP1) as crucial cellular receptor

Julian Schoellkopf,
Thomas Mueller,
Lena Hippchen,
Teresa Mueller,
Raphael Reuten,
Rolf Backofen,
Joachim Orth,
Gudula Schmidt

Published: December 14, 2022
https://doi.org/10.1371/journal.ppat.1010781

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Toxins
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Cell binding
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Intoxication
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Pasteurella multocida
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CRISPR
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Binding analysis
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Endocytosis
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