In vitro evolution predicts emerging SARS-CoV-2 mutations with high affinity for ACE2 and cross-species binding

• Neil Bate,

  Roles Formal analysis, Investigation, Writing – original draft, Writing – review & editing

  Affiliations Department of Molecular & Cell Biology, University of Leicester, Leicester, Leicester United Kingdom, Department of Cardiovascular Sciences, University of Leicester, Leicester, Leicester United Kingdom

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• Christos G. Savva,

  Roles Formal analysis, Investigation, Visualization, Writing – original draft, Writing – review & editing

  Affiliation Leicester Institute of Structural and Chemical Biology, Department of Molecular and Cellular Biology, University of Leicester, Leicester, United Kingdom

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• Peter C. E. Moody,

  Roles Conceptualization, Formal analysis, Writing – original draft, Writing – review & editing

  Affiliation Leicester Institute of Structural and Chemical Biology, Department of Molecular and Cellular Biology, University of Leicester, Leicester, United Kingdom

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• Edward A. Brown,

  Roles Investigation, Writing – review & editing

  Affiliation Leicester Institute of Structural and Chemical Biology, Department of Molecular and Cellular Biology, University of Leicester, Leicester, United Kingdom

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• Sian E. Evans,

  Roles Investigation, Writing – review & editing

  Affiliation Department of Molecular & Cell Biology, University of Leicester, Leicester, Leicester United Kingdom

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• Jonathan K. Ball,

  Roles Conceptualization, Writing – original draft, Writing – review & editing

  Affiliation School of Life Sciences, The University of Nottingham, Nottingham United Kingdom

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• John W. R. Schwabe,

  Roles Conceptualization, Writing – original draft, Writing – review & editing

  Affiliation Leicester Institute of Structural and Chemical Biology, Department of Molecular and Cellular Biology, University of Leicester, Leicester, United Kingdom

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• Julian E. Sale,

  Roles Conceptualization, Supervision, Writing – original draft, Writing – review & editing

  Affiliation Division of Protein & Nucleic Acid Chemistry, MRC Laboratory of Molecular Biology, Cambridge, United Kingdom

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• Nicholas P. J. Brindle

  Roles Conceptualization, Formal analysis, Funding acquisition, Investigation, Project administration, Supervision, Visualization, Writing – original draft, Writing – review & editing

  * E-mail: NPJB1@Le.ac.uk

  Affiliations Department of Molecular & Cell Biology, University of Leicester, Leicester, Leicester United Kingdom, Department of Cardiovascular Sciences, University of Leicester, Leicester, Leicester United Kingdom

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