Engineered disulfide reveals structural dynamics of locked SARS-CoV-2 spike

Kun Qu; Qiuluan Chen; Katarzyna A. Ciazynska; Banghui Liu; Xixi Zhang; Jingjing Wang; Yujie He; Jiali Guan; Jun He; Tian Liu; Xiaofei Zhang; Andrew P. Carter; Xiaoli Xiong; John A. G. Briggs

doi:10.1371/journal.ppat.1010583

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Open Access

Peer-reviewed

Research Article

Engineered disulfide reveals structural dynamics of locked SARS-CoV-2 spike

Kun Qu ,

Contributed equally to this work with: Kun Qu, Qiuluan Chen

Roles Conceptualization, Investigation, Methodology, Visualization, Writing – original draft

Affiliations Structural Studies Division, Medical Research Council Laboratory of Molecular Biology, Cambridge, United Kingdom, Infectious Diseases Translational Research Programme, Department of Biochemistry, Yong Loo Lin School of Medicine, National University of Singapore, Singapore

https://orcid.org/0000-0002-3152-2237

⨯
Qiuluan Chen ,

Contributed equally to this work with: Kun Qu, Qiuluan Chen

Roles Investigation, Methodology, Visualization, Writing – review & editing

Affiliation Bioland Laboratory (Guangzhou Regenerative Medicine and Health—Guangdong Laboratory), Guangzhou, China
⨯
Katarzyna A. Ciazynska,

Roles Investigation

Affiliation Structural Studies Division, Medical Research Council Laboratory of Molecular Biology, Cambridge, United Kingdom

https://orcid.org/0000-0002-9899-2428

⨯
Banghui Liu,

Roles Investigation

Affiliation The State Key Laboratory of Respiratory Disease (SKLRD), CAS Key Laboratory of Regenerative Biology, Guangdong Provincial Key Laboratory of Stem Cell and Regenerative Medicine, Center for Cell Lineage and Development, Guangzhou Institutes of Biomedicine and Health, Chinese Academy of Sciences, Guangzhou, China
⨯
Xixi Zhang,

Roles Investigation

Affiliation The State Key Laboratory of Respiratory Disease (SKLRD), CAS Key Laboratory of Regenerative Biology, Guangdong Provincial Key Laboratory of Stem Cell and Regenerative Medicine, Center for Cell Lineage and Development, Guangzhou Institutes of Biomedicine and Health, Chinese Academy of Sciences, Guangzhou, China
⨯
Jingjing Wang,

Roles Investigation

Affiliation The State Key Laboratory of Respiratory Disease (SKLRD), CAS Key Laboratory of Regenerative Biology, Guangdong Provincial Key Laboratory of Stem Cell and Regenerative Medicine, Center for Cell Lineage and Development, Guangzhou Institutes of Biomedicine and Health, Chinese Academy of Sciences, Guangzhou, China
⨯
Yujie He,

Roles Investigation

Affiliation Bioland Laboratory (Guangzhou Regenerative Medicine and Health—Guangdong Laboratory), Guangzhou, China
⨯
Jiali Guan,

Roles Investigation

Affiliation Bioland Laboratory (Guangzhou Regenerative Medicine and Health—Guangdong Laboratory), Guangzhou, China
⨯
Jun He,

Roles Investigation

Affiliations Bioland Laboratory (Guangzhou Regenerative Medicine and Health—Guangdong Laboratory), Guangzhou, China, The State Key Laboratory of Respiratory Disease (SKLRD), CAS Key Laboratory of Regenerative Biology, Guangdong Provincial Key Laboratory of Stem Cell and Regenerative Medicine, Center for Cell Lineage and Development, Guangzhou Institutes of Biomedicine and Health, Chinese Academy of Sciences, Guangzhou, China
⨯
Tian Liu,

Roles Investigation

Affiliation Center for Proteomics and Metabolomics, Bioland Laboratory, Guangzhou Regenerative Medicine and Health Guangdong Laboratory, Guangzhou, China
⨯
Xiaofei Zhang,

Roles Investigation

Affiliations Bioland Laboratory (Guangzhou Regenerative Medicine and Health—Guangdong Laboratory), Guangzhou, China, The State Key Laboratory of Respiratory Disease (SKLRD), CAS Key Laboratory of Regenerative Biology, Guangdong Provincial Key Laboratory of Stem Cell and Regenerative Medicine, Center for Cell Lineage and Development, Guangzhou Institutes of Biomedicine and Health, Chinese Academy of Sciences, Guangzhou, China, Center for Proteomics and Metabolomics, Bioland Laboratory, Guangzhou Regenerative Medicine and Health Guangdong Laboratory, Guangzhou, China
⨯
Andrew P. Carter,

Roles Funding acquisition, Resources, Writing – review & editing

Affiliation Structural Studies Division, Medical Research Council Laboratory of Molecular Biology, Cambridge, United Kingdom

https://orcid.org/0000-0001-7292-5430

⨯
Xiaoli Xiong ,

Roles Conceptualization, Funding acquisition, Investigation, Methodology, Project administration, Resources, Supervision, Visualization, Writing – original draft

* E-mail: xiong_xiaoli@gibh.ac.cn (XX); briggs@biochem.mpg.de (JAGB)

Affiliations Structural Studies Division, Medical Research Council Laboratory of Molecular Biology, Cambridge, United Kingdom, Bioland Laboratory (Guangzhou Regenerative Medicine and Health—Guangdong Laboratory), Guangzhou, China, The State Key Laboratory of Respiratory Disease (SKLRD), CAS Key Laboratory of Regenerative Biology, Guangdong Provincial Key Laboratory of Stem Cell and Regenerative Medicine, Center for Cell Lineage and Development, Guangzhou Institutes of Biomedicine and Health, Chinese Academy of Sciences, Guangzhou, China

https://orcid.org/0000-0002-4632-9122

⨯
John A. G. Briggs

Roles Conceptualization, Funding acquisition, Methodology, Project administration, Resources, Supervision, Visualization, Writing – original draft

* E-mail: xiong_xiaoli@gibh.ac.cn (XX); briggs@biochem.mpg.de (JAGB)

Affiliations Structural Studies Division, Medical Research Council Laboratory of Molecular Biology, Cambridge, United Kingdom, Max Planck Institute of Biochemistry, Martinsried, Germany

https://orcid.org/0000-0003-3990-6910

⨯

Engineered disulfide reveals structural dynamics of locked SARS-CoV-2 spike

Kun Qu,
Qiuluan Chen,
Katarzyna A. Ciazynska,
Banghui Liu,
Xixi Zhang,
Jingjing Wang,
Yujie He,
Jiali Guan,
Jun He,
Tian Liu

Published: July 29, 2022
https://doi.org/10.1371/journal.ppat.1010583

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Disulfide bonds
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