Quantitative regulation of the thermal stability of enveloped virus vaccines by surface charge engineering to prevent the self-aggregation of attachment glycoproteins

Yu Shang; Li Li; Tengfei Zhang; Qingping Luo; Qingzhong Yu; Zhe Zeng; Lintao Li; Miaomiao Jia; Guoyi Tang; Sanlin Fan; Qin Lu; Wenting Zhang; Yuhan Xue; Hongling Wang; Wei Liu; Hongcai Wang; Rongrong Zhang; Chan Ding; Huabin Shao; Guoyuan Wen

doi:10.1371/journal.ppat.1010564

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Open Access

Peer-reviewed

Research Article

Quantitative regulation of the thermal stability of enveloped virus vaccines by surface charge engineering to prevent the self-aggregation of attachment glycoproteins

Yu Shang ,

Contributed equally to this work with: Yu Shang, Li Li

Roles Conceptualization, Investigation, Writing – original draft

Affiliations Institute of Animal Husbandry and Veterinary Sciences, Hubei Academy of Agricultural Sciences, Wuhan, China, Key Laboratory of Prevention and Control Agents for Animal Bacteriosis (Ministry of Agriculture), Wuhan, China
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Li Li ,

Contributed equally to this work with: Yu Shang, Li Li

Roles Data curation, Investigation

Affiliations Institute of Animal Husbandry and Veterinary Sciences, Hubei Academy of Agricultural Sciences, Wuhan, China, Key Laboratory of Prevention and Control Agents for Animal Bacteriosis (Ministry of Agriculture), Wuhan, China
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Tengfei Zhang,

Roles Data curation, Methodology, Writing – original draft

Affiliations Institute of Animal Husbandry and Veterinary Sciences, Hubei Academy of Agricultural Sciences, Wuhan, China, Key Laboratory of Prevention and Control Agents for Animal Bacteriosis (Ministry of Agriculture), Wuhan, China
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Qingping Luo,

Roles Data curation, Formal analysis, Investigation, Supervision

Affiliations Institute of Animal Husbandry and Veterinary Sciences, Hubei Academy of Agricultural Sciences, Wuhan, China, Key Laboratory of Prevention and Control Agents for Animal Bacteriosis (Ministry of Agriculture), Wuhan, China
⨯
Qingzhong Yu,

Roles Conceptualization, Writing – review & editing

Affiliation US National Poultry Research Center, Agricultural Research Services, United States Department of Agriculture, Athens, Georgia, United States of America
⨯
Zhe Zeng,

Roles Methodology

Affiliations Institute of Animal Husbandry and Veterinary Sciences, Hubei Academy of Agricultural Sciences, Wuhan, China, Key Laboratory of Prevention and Control Agents for Animal Bacteriosis (Ministry of Agriculture), Wuhan, China
⨯
Lintao Li,

Roles Investigation

Affiliations Institute of Animal Husbandry and Veterinary Sciences, Hubei Academy of Agricultural Sciences, Wuhan, China, Key Laboratory of Prevention and Control Agents for Animal Bacteriosis (Ministry of Agriculture), Wuhan, China
⨯
Miaomiao Jia,

Roles Investigation

Affiliations Institute of Animal Husbandry and Veterinary Sciences, Hubei Academy of Agricultural Sciences, Wuhan, China, Key Laboratory of Prevention and Control Agents for Animal Bacteriosis (Ministry of Agriculture), Wuhan, China
⨯
Guoyi Tang,

Roles Investigation

Affiliations Institute of Animal Husbandry and Veterinary Sciences, Hubei Academy of Agricultural Sciences, Wuhan, China, Key Laboratory of Prevention and Control Agents for Animal Bacteriosis (Ministry of Agriculture), Wuhan, China
⨯
Sanlin Fan,

Roles Investigation

Affiliations Institute of Animal Husbandry and Veterinary Sciences, Hubei Academy of Agricultural Sciences, Wuhan, China, Key Laboratory of Prevention and Control Agents for Animal Bacteriosis (Ministry of Agriculture), Wuhan, China
⨯
Qin Lu,

Roles Investigation

Affiliations Institute of Animal Husbandry and Veterinary Sciences, Hubei Academy of Agricultural Sciences, Wuhan, China, Key Laboratory of Prevention and Control Agents for Animal Bacteriosis (Ministry of Agriculture), Wuhan, China
⨯
Wenting Zhang,

Roles Formal analysis, Methodology

Affiliations Institute of Animal Husbandry and Veterinary Sciences, Hubei Academy of Agricultural Sciences, Wuhan, China, Key Laboratory of Prevention and Control Agents for Animal Bacteriosis (Ministry of Agriculture), Wuhan, China
⨯
Yuhan Xue,

Roles Investigation

Affiliations Institute of Animal Husbandry and Veterinary Sciences, Hubei Academy of Agricultural Sciences, Wuhan, China, Key Laboratory of Prevention and Control Agents for Animal Bacteriosis (Ministry of Agriculture), Wuhan, China
⨯
Hongling Wang,

Roles Investigation

Affiliations Institute of Animal Husbandry and Veterinary Sciences, Hubei Academy of Agricultural Sciences, Wuhan, China, Key Laboratory of Prevention and Control Agents for Animal Bacteriosis (Ministry of Agriculture), Wuhan, China
⨯
Wei Liu,

Roles Investigation

Affiliations Institute of Animal Husbandry and Veterinary Sciences, Hubei Academy of Agricultural Sciences, Wuhan, China, Key Laboratory of Prevention and Control Agents for Animal Bacteriosis (Ministry of Agriculture), Wuhan, China
⨯
Hongcai Wang,

Roles Investigation

Affiliations Institute of Animal Husbandry and Veterinary Sciences, Hubei Academy of Agricultural Sciences, Wuhan, China, Key Laboratory of Prevention and Control Agents for Animal Bacteriosis (Ministry of Agriculture), Wuhan, China
⨯
Rongrong Zhang,

Roles Investigation

Affiliations Institute of Animal Husbandry and Veterinary Sciences, Hubei Academy of Agricultural Sciences, Wuhan, China, Key Laboratory of Prevention and Control Agents for Animal Bacteriosis (Ministry of Agriculture), Wuhan, China
⨯
Chan Ding,

Roles Writing – review & editing

Affiliation Department of Avian Diseases, Shanghai Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Shanghai, China
⨯
Huabin Shao,

Roles Conceptualization, Funding acquisition, Supervision, Writing – review & editing

Affiliations Institute of Animal Husbandry and Veterinary Sciences, Hubei Academy of Agricultural Sciences, Wuhan, China, Key Laboratory of Prevention and Control Agents for Animal Bacteriosis (Ministry of Agriculture), Wuhan, China, Hubei Provincial Key Laboratory of Animal Pathogenic Microbiology, Wuhan, China
⨯
[ ... ],
Guoyuan Wen

Roles Conceptualization, Formal analysis, Funding acquisition, Supervision, Writing – original draft, Writing – review & editing

* E-mail: wgy_524@163.com

Affiliations Institute of Animal Husbandry and Veterinary Sciences, Hubei Academy of Agricultural Sciences, Wuhan, China, Key Laboratory of Prevention and Control Agents for Animal Bacteriosis (Ministry of Agriculture), Wuhan, China, Hubei Provincial Key Laboratory of Animal Pathogenic Microbiology, Wuhan, China

https://orcid.org/0000-0003-3580-6963

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Quantitative regulation of the thermal stability of enveloped virus vaccines by surface charge engineering to prevent the self-aggregation of attachment glycoproteins

Yu Shang,
Li Li,
Tengfei Zhang,
Qingping Luo,
Qingzhong Yu,
Zhe Zeng,
Lintao Li,
Miaomiao Jia,
Guoyi Tang,
Sanlin Fan

Published: June 9, 2022
https://doi.org/10.1371/journal.ppat.1010564

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Thermal stability
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Protein structure
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