SARS-CoV-2 variants with mutations at the S1/S2 cleavage site are generated in vitro during propagation in TMPRSS2-deficient cells

• Michihito Sasaki ,

  Roles Conceptualization, Data curation, Formal analysis, Investigation, Resources, Validation, Visualization, Writing – original draft

  * E-mail: m-sasaki@czc.hokudai.ac.jp

  Affiliation Division of Molecular Pathobiology, Research Center for Zoonosis Control, Hokkaido University, Sapporo, Japan

  https://orcid.org/0000-0003-1607-2175

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• Kentaro Uemura,

  Roles Data curation, Formal analysis, Investigation, Validation, Writing – review & editing

  Affiliations Division of Molecular Pathobiology, Research Center for Zoonosis Control, Hokkaido University, Sapporo, Japan, Shionogi & Co., Ltd., Osaka, Japan, Laboratory of Biomolecular Science, Faculty of Pharmaceutical Science, Hokkaido University, Sapporo, Japan

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• Akihiko Sato,

  Roles Conceptualization, Formal analysis, Investigation, Project administration, Validation

  Affiliations Division of Molecular Pathobiology, Research Center for Zoonosis Control, Hokkaido University, Sapporo, Japan, Shionogi & Co., Ltd., Osaka, Japan

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• Shinsuke Toba,

  Roles Formal analysis, Investigation, Validation

  Affiliations Division of Molecular Pathobiology, Research Center for Zoonosis Control, Hokkaido University, Sapporo, Japan, Shionogi & Co., Ltd., Osaka, Japan

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• Takao Sanaki,

  Roles Resources

  Affiliations Division of Molecular Pathobiology, Research Center for Zoonosis Control, Hokkaido University, Sapporo, Japan, Shionogi & Co., Ltd., Osaka, Japan

  https://orcid.org/0000-0001-8847-0071

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• Katsumi Maenaka,

  Roles Resources

  Affiliations Laboratory of Biomolecular Science, Faculty of Pharmaceutical Science, Hokkaido University, Sapporo, Japan, Global Institution for Collaborative Research and Education, Hokkaido University, Sapporo, Japan

  https://orcid.org/0000-0002-5459-521X

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• William W. Hall,

  Roles Writing – review & editing

  Affiliations International Collaboration Unit, Research Center for Zoonosis Control, Hokkaido University, Sapporo, Japan, National Virus Reference Laboratory, School of Medicine, University College of Dublin, Ireland, Global Virus Network, Baltimore, Maryland, United States of America

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• Yasuko Orba,

  Roles Resources, Writing – review & editing

  Affiliations Division of Molecular Pathobiology, Research Center for Zoonosis Control, Hokkaido University, Sapporo, Japan, International Collaboration Unit, Research Center for Zoonosis Control, Hokkaido University, Sapporo, Japan

  https://orcid.org/0000-0001-9910-3912

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• Hirofumi Sawa

  Roles Conceptualization, Project administration, Resources, Supervision, Writing – review & editing

  Affiliations Division of Molecular Pathobiology, Research Center for Zoonosis Control, Hokkaido University, Sapporo, Japan, International Collaboration Unit, Research Center for Zoonosis Control, Hokkaido University, Sapporo, Japan, Global Virus Network, Baltimore, Maryland, United States of America

  https://orcid.org/0000-0003-2569-2755

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