Lipid droplets fuel SARS-CoV-2 replication and production of inflammatory mediators

Suelen Silva Gomes Dias; Vinicius Cardoso Soares; André C. Ferreira; Carolina Q. Sacramento; Natalia Fintelman-Rodrigues; Jairo R. Temerozo; Lívia Teixeira; Marcos Alexandre Nunes da Silva; Ester Barreto; Mayara Mattos; Caroline S. de Freitas; Isaclaudia G. Azevedo-Quintanilha; Pedro Paulo A. Manso; Milene D. Miranda; Marilda Mendonça Siqueira; Eugenio D. Hottz; Camila R. R. Pão; Dumith C. Bou-Habib; Debora F. Barreto-Vieira; Fernando A. Bozza; Thiago M. L. Souza; Patricia T. Bozza

doi:10.1371/journal.ppat.1009127

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Open Access

Peer-reviewed

Research Article

Lipid droplets fuel SARS-CoV-2 replication and production of inflammatory mediators

Suelen Silva Gomes Dias ,

Contributed equally to this work with: Suelen Silva Gomes Dias, Vinicius Cardoso Soares

Roles Conceptualization, Data curation, Formal analysis, Investigation, Methodology, Validation, Visualization, Writing – original draft, Writing – review & editing

Affiliation Laboratório de Imunofarmacologia, Instituto Oswaldo Cruz (IOC), Fundação Oswaldo Cruz (FIOCRUZ), Rio de Janeiro, Rio de Janeiro, Brazil

https://orcid.org/0000-0002-3104-0983

⨯
Vinicius Cardoso Soares ,

Contributed equally to this work with: Suelen Silva Gomes Dias, Vinicius Cardoso Soares

Roles Conceptualization, Data curation, Formal analysis, Investigation, Methodology, Validation, Visualization, Writing – original draft, Writing – review & editing

Affiliations Laboratório de Imunofarmacologia, Instituto Oswaldo Cruz (IOC), Fundação Oswaldo Cruz (FIOCRUZ), Rio de Janeiro, Rio de Janeiro, Brazil, Programa de Imunologia e Inflamação, Universidade Federal do Rio de Janeiro, UFRJ, Rio de Janeiro, Rio de Janeiro, Brazil

https://orcid.org/0000-0002-0552-5995

⨯
André C. Ferreira,

Roles Investigation, Methodology, Validation, Writing – review & editing

Affiliations Laboratório de Imunofarmacologia, Instituto Oswaldo Cruz (IOC), Fundação Oswaldo Cruz (FIOCRUZ), Rio de Janeiro, Rio de Janeiro, Brazil, Centro De Desenvolvimento Tecnológico Em Saúde (CDTS) and National Institute for Science and Technology on Innovation on Diseases of Neglected Populations (INCT/IDNP), FIOCRUZ, Rio de Janeiro, Brasil, Universidade Iguaçu, Nova Iguaçu, Rio de Janeiro, Brazil

https://orcid.org/0000-0002-9064-2351

⨯
Carolina Q. Sacramento,

Roles Data curation, Investigation, Methodology, Validation, Writing – review & editing

Affiliations Laboratório de Imunofarmacologia, Instituto Oswaldo Cruz (IOC), Fundação Oswaldo Cruz (FIOCRUZ), Rio de Janeiro, Rio de Janeiro, Brazil, Centro De Desenvolvimento Tecnológico Em Saúde (CDTS) and National Institute for Science and Technology on Innovation on Diseases of Neglected Populations (INCT/IDNP), FIOCRUZ, Rio de Janeiro, Brasil

https://orcid.org/0000-0003-3952-7099

⨯
Natalia Fintelman-Rodrigues,

Roles Investigation, Methodology, Writing – review & editing

Affiliations Laboratório de Imunofarmacologia, Instituto Oswaldo Cruz (IOC), Fundação Oswaldo Cruz (FIOCRUZ), Rio de Janeiro, Rio de Janeiro, Brazil, Centro De Desenvolvimento Tecnológico Em Saúde (CDTS) and National Institute for Science and Technology on Innovation on Diseases of Neglected Populations (INCT/IDNP), FIOCRUZ, Rio de Janeiro, Brasil
⨯
Jairo R. Temerozo,

Roles Data curation, Investigation, Methodology, Validation, Writing – review & editing

Affiliation Laboratório de Pesquisas sobre o Timo and Instituto National de Ciencia e Tecnologia em Neuroimunomodulação (INCT/NIM), Instituto Oswaldo Cruz (FIOCRUZ), Rio de Janeiro, Rio de Janeiro, Brazil

https://orcid.org/0000-0002-8092-2149

⨯
Lívia Teixeira,

Roles Conceptualization, Investigation, Methodology, Validation, Writing – review & editing

Affiliation Laboratório de Imunofarmacologia, Instituto Oswaldo Cruz (IOC), Fundação Oswaldo Cruz (FIOCRUZ), Rio de Janeiro, Rio de Janeiro, Brazil

https://orcid.org/0000-0002-6227-3202

⨯
Marcos Alexandre Nunes da Silva,

Roles Investigation, Methodology, Writing – review & editing

Affiliation Laboratório de Morfologia e Morfogênese Viral, Instituto Oswaldo Cruz (IOC), Fundação Oswaldo Cruz (FIOCRUZ), Rio de Janeiro, Rio de Janeiro, Brazil
⨯
Ester Barreto,

Roles Investigation, Methodology, Writing – review & editing

Affiliation Laboratório de Imunofarmacologia, Instituto Oswaldo Cruz (IOC), Fundação Oswaldo Cruz (FIOCRUZ), Rio de Janeiro, Rio de Janeiro, Brazil

https://orcid.org/0000-0002-1982-5841

⨯
Mayara Mattos,

Roles Investigation, Methodology, Writing – review & editing

Affiliations Laboratório de Imunofarmacologia, Instituto Oswaldo Cruz (IOC), Fundação Oswaldo Cruz (FIOCRUZ), Rio de Janeiro, Rio de Janeiro, Brazil, Centro De Desenvolvimento Tecnológico Em Saúde (CDTS) and National Institute for Science and Technology on Innovation on Diseases of Neglected Populations (INCT/IDNP), FIOCRUZ, Rio de Janeiro, Brasil

https://orcid.org/0000-0002-6841-6999

⨯
Caroline S. de Freitas,

Roles Investigation, Methodology, Writing – review & editing

Affiliations Laboratório de Imunofarmacologia, Instituto Oswaldo Cruz (IOC), Fundação Oswaldo Cruz (FIOCRUZ), Rio de Janeiro, Rio de Janeiro, Brazil, Centro De Desenvolvimento Tecnológico Em Saúde (CDTS) and National Institute for Science and Technology on Innovation on Diseases of Neglected Populations (INCT/IDNP), FIOCRUZ, Rio de Janeiro, Brasil

https://orcid.org/0000-0001-8100-0472

⨯
Isaclaudia G. Azevedo-Quintanilha,

Roles Investigation, Methodology, Writing – review & editing

Affiliation Laboratório de Imunofarmacologia, Instituto Oswaldo Cruz (IOC), Fundação Oswaldo Cruz (FIOCRUZ), Rio de Janeiro, Rio de Janeiro, Brazil
⨯
Pedro Paulo A. Manso,

Roles Investigation, Methodology, Visualization, Writing – review & editing

Affiliation Laboratorio de Patologia, Instituto Oswaldo Cruz (IOC), Fundação Oswaldo Cruz (FIOCRUZ), Rio de Janeiro, Rio de Janeiro, Brazil

https://orcid.org/0000-0002-7255-0429

⨯
Milene D. Miranda,

Roles Methodology, Writing – review & editing

Affiliation Laboratório de Vírus Respiratórios e do Sarampo, Instituto Oswaldo Cruz (FIOCRUZ), Rio de Janeiro, Rio de Janeiro, Brazil

https://orcid.org/0000-0003-0037-059X

⨯
Marilda Mendonça Siqueira,

Roles Investigation, Resources, Writing – review & editing

Affiliation Laboratório de Vírus Respiratórios e do Sarampo, Instituto Oswaldo Cruz (FIOCRUZ), Rio de Janeiro, Rio de Janeiro, Brazil

https://orcid.org/0000-0003-4685-9817

⨯
Eugenio D. Hottz,

Roles Investigation, Methodology, Writing – review & editing

Affiliations Laboratório de Imunofarmacologia, Instituto Oswaldo Cruz (IOC), Fundação Oswaldo Cruz (FIOCRUZ), Rio de Janeiro, Rio de Janeiro, Brazil, Laboratorio de Imunotrombose, Departamento de Bioquímica, Universidade Federal de Juiz de Fora, Juiz de Fora, Minas Gerais, Brazil

https://orcid.org/0000-0002-2201-1742

⨯
Camila R. R. Pão,

Roles Methodology, Writing – review & editing

Affiliation Laboratório de Imunofarmacologia, Instituto Oswaldo Cruz (IOC), Fundação Oswaldo Cruz (FIOCRUZ), Rio de Janeiro, Rio de Janeiro, Brazil

https://orcid.org/0000-0001-7668-4195

⨯
Dumith C. Bou-Habib,

Roles Funding acquisition, Investigation, Supervision, Writing – original draft, Writing – review & editing

Affiliation Laboratório de Pesquisas sobre o Timo and Instituto National de Ciencia e Tecnologia em Neuroimunomodulação (INCT/NIM), Instituto Oswaldo Cruz (FIOCRUZ), Rio de Janeiro, Rio de Janeiro, Brazil

https://orcid.org/0000-0003-0552-9045

⨯
Debora F. Barreto-Vieira,

Roles Investigation, Methodology, Supervision, Visualization, Writing – review & editing

Affiliation Laboratório de Morfologia e Morfogênese Viral, Instituto Oswaldo Cruz (IOC), Fundação Oswaldo Cruz (FIOCRUZ), Rio de Janeiro, Rio de Janeiro, Brazil
⨯
Fernando A. Bozza,

Roles Conceptualization, Funding acquisition, Investigation, Project administration, Resources, Supervision, Writing – review & editing

Affiliations Instituto Nacional de Infectologia Evandro Chagas (INI), FIOCRUZ, Rio de Janeiro, Brazil, Instituto D’Or de Pesquisa e Ensino (IDOR), Rio de Janeiro, Brazil

https://orcid.org/0000-0003-4878-0256

⨯
Thiago M. L. Souza,

Roles Conceptualization, Formal analysis, Funding acquisition, Investigation, Project administration, Resources, Supervision, Writing – original draft, Writing – review & editing

Affiliations Laboratório de Imunofarmacologia, Instituto Oswaldo Cruz (IOC), Fundação Oswaldo Cruz (FIOCRUZ), Rio de Janeiro, Rio de Janeiro, Brazil, Centro De Desenvolvimento Tecnológico Em Saúde (CDTS) and National Institute for Science and Technology on Innovation on Diseases of Neglected Populations (INCT/IDNP), FIOCRUZ, Rio de Janeiro, Brasil

https://orcid.org/0000-0003-2212-3899

⨯
[ ... ],
Patricia T. Bozza

Roles Conceptualization, Formal analysis, Funding acquisition, Investigation, Project administration, Resources, Supervision, Writing – original draft, Writing – review & editing

* E-mail: pbozza@ioc.fiocruz.br, pbozza@gmail.com

Affiliation Laboratório de Imunofarmacologia, Instituto Oswaldo Cruz (IOC), Fundação Oswaldo Cruz (FIOCRUZ), Rio de Janeiro, Rio de Janeiro, Brazil

https://orcid.org/0000-0001-8349-9529

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Lipid droplets fuel SARS-CoV-2 replication and production of inflammatory mediators

Suelen Silva Gomes Dias,
Vinicius Cardoso Soares,
André C. Ferreira,
Carolina Q. Sacramento,
Natalia Fintelman-Rodrigues,
Jairo R. Temerozo,
Lívia Teixeira,
Marcos Alexandre Nunes da Silva,
Ester Barreto,
Mayara Mattos

Published: December 16, 2020
https://doi.org/10.1371/journal.ppat.1009127

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18 Dec 2020
13:36 GMT
Most Recent

Problem with EM interpretation Posted by proingeard on 18 Dec 2020 at 13:36 GMT

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