Highly efficient CRISPR-Cas9-mediated gene knockout in primary human B cells for functional genetic studies of Epstein-Barr virus infection

• Ezgi Akidil ,

  Roles Formal analysis, Investigation, Methodology, Software, Validation, Visualization, Writing – original draft, Writing – review & editing

  ¶‡ These authors share first authorship on this work.

  Affiliations Research Unit Gene Vectors, Helmholtz Zentrum München, German Research Center for Environmental Health, Munich, Germany, German Center for Infection Research (DZIF), Partner site Munich, Munich, Germany

  https://orcid.org/0000-0001-6873-1916

  ⨯
• Manuel Albanese ,

  Roles Conceptualization, Formal analysis, Investigation, Methodology, Software, Validation, Visualization, Writing – original draft, Writing – review & editing

  ¶‡ These authors share first authorship on this work.

  Affiliations Research Unit Gene Vectors, Helmholtz Zentrum München, German Research Center for Environmental Health, Munich, Germany, German Center for Infection Research (DZIF), Partner site Munich, Munich, Germany, Max von Pettenkofer Institute and Gene Center, Virology, National Reference Center for Retroviruses, Faculty of Medicine, LMU München, Munich, Germany

  https://orcid.org/0000-0002-4614-7599

  ⨯
• Alexander Buschle,

  Roles Data curation, Formal analysis, Investigation, Methodology, Software, Supervision, Validation, Visualization, Writing – original draft, Writing – review & editing

  Affiliations Research Unit Gene Vectors, Helmholtz Zentrum München, German Research Center for Environmental Health, Munich, Germany, German Center for Infection Research (DZIF), Partner site Munich, Munich, Germany

  https://orcid.org/0000-0001-6126-514X

  ⨯
• Adrian Ruhle,

  Roles Investigation, Methodology

  Affiliations German Center for Infection Research (DZIF), Partner site Munich, Munich, Germany, Max von Pettenkofer Institute and Gene Center, Virology, National Reference Center for Retroviruses, Faculty of Medicine, LMU München, Munich, Germany

  https://orcid.org/0000-0002-8132-9814

  ⨯
• Dagmar Pich,

  Roles Investigation

  Affiliations Research Unit Gene Vectors, Helmholtz Zentrum München, German Research Center for Environmental Health, Munich, Germany, German Center for Infection Research (DZIF), Partner site Munich, Munich, Germany

  ⨯
• Oliver T. Keppler,

  Roles Resources, Supervision, Writing – original draft

  Affiliations German Center for Infection Research (DZIF), Partner site Munich, Munich, Germany, Max von Pettenkofer Institute and Gene Center, Virology, National Reference Center for Retroviruses, Faculty of Medicine, LMU München, Munich, Germany

  https://orcid.org/0000-0002-1384-8946

  ⨯
• Wolfgang Hammerschmidt

  Roles Conceptualization, Funding acquisition, Investigation, Project administration, Resources, Supervision, Validation, Writing – original draft, Writing – review & editing

  * E-mail: hammerschmidt@helmholtz-muenchen.de

  Affiliations Research Unit Gene Vectors, Helmholtz Zentrum München, German Research Center for Environmental Health, Munich, Germany, German Center for Infection Research (DZIF), Partner site Munich, Munich, Germany

  https://orcid.org/0000-0002-4659-0427

  ⨯