Antigenic Characterization of the HCMV gH/gL/gO and Pentamer Cell Entry Complexes Reveals Binding Sites for Potently Neutralizing Human Antibodies

Claudio Ciferri; Sumana Chandramouli; Alexander Leitner; Danilo Donnarumma; Michael A. Cianfrocco; Rachel Gerrein; Kristian Friedrich; Yukti Aggarwal; Giuseppe Palladino; Ruedi Aebersold; Nathalie Norais; Ethan C. Settembre; Andrea Carfi

doi:10.1371/journal.ppat.1005230

Loading metrics

Open Access

Peer-reviewed

Research Article

Antigenic Characterization of the HCMV gH/gL/gO and Pentamer Cell Entry Complexes Reveals Binding Sites for Potently Neutralizing Human Antibodies

Claudio Ciferri ,

* E-mail: ciferric@gene.com (CC); andrea.x.carfi@gsk.com (AC)

Current Address: Genentech Inc., South San Francisco, California, United States of America

Affiliation Novartis Vaccines (a GSK company), Cambridge, Massachusetts, United States of America
⨯
Sumana Chandramouli,

Affiliation Novartis Vaccines (a GSK company), Cambridge, Massachusetts, United States of America
⨯
Alexander Leitner,

Affiliation ETH Zürich, Department of Biology, Institute of Molecular Systems Biology, Zürich, Switzerland
⨯
Danilo Donnarumma,

Affiliation Novartis Vaccines (a GSK company), Siena, Italy
⨯
Michael A. Cianfrocco,

Affiliations Harvard University, Department of Molecular and Cellular Biology, Cambridge, Massachusetts, United States of America, Harvard Medical School, Department of Cell Biology, Boston, Massachusetts, United States of America
⨯
Rachel Gerrein,

Affiliation Novartis Vaccines (a GSK company), Cambridge, Massachusetts, United States of America
⨯
Kristian Friedrich,

Affiliation Novartis Vaccines (a GSK company), Cambridge, Massachusetts, United States of America
⨯
Yukti Aggarwal,

Affiliation Novartis Vaccines (a GSK company), Cambridge, Massachusetts, United States of America
⨯
Giuseppe Palladino,

Current Address: Novartis Influenza Vaccines, Cambridge, Massachusetts, United States of America

Affiliation Novartis Vaccines (a GSK company), Cambridge, Massachusetts, United States of America
⨯
Ruedi Aebersold,

Affiliations ETH Zürich, Department of Biology, Institute of Molecular Systems Biology, Zürich, Switzerland, Faculty of Science, University of Zurich, Zurich, Switzerland
⨯
Nathalie Norais,

Affiliation Novartis Vaccines (a GSK company), Siena, Italy
⨯
Ethan C. Settembre,

Current Address: Novartis Influenza Vaccines, Cambridge, Massachusetts, United States of America

Affiliation Novartis Vaccines (a GSK company), Cambridge, Massachusetts, United States of America
⨯
Andrea Carfi

* E-mail: ciferric@gene.com (CC); andrea.x.carfi@gsk.com (AC)

Affiliation Novartis Vaccines (a GSK company), Cambridge, Massachusetts, United States of America
⨯

Antigenic Characterization of the HCMV gH/gL/gO and Pentamer Cell Entry Complexes Reveals Binding Sites for Potently Neutralizing Human Antibodies

Claudio Ciferri,
Sumana Chandramouli,
Alexander Leitner,
Danilo Donnarumma,
Michael A. Cianfrocco,
Rachel Gerrein,
Kristian Friedrich,
Yukti Aggarwal,
Giuseppe Palladino,
Ruedi Aebersold

Published: October 20, 2015
https://doi.org/10.1371/journal.ppat.1005230

Reader Comments (0)

Post a new comment on this article

Subject Areas
?

For more information about PLOS Subject Areas, click here.
We want your feedback. Do these Subject Areas make sense for this article? Click the target next to the incorrect Subject Area and let us know. Thanks for your help!

Antibodies
Is the Subject Area "Antibodies" applicable to this article?

Thanks for your feedback.
Monoclonal antibodies
Is the Subject Area "Monoclonal antibodies" applicable to this article?

Thanks for your feedback.
Enzyme-linked immunoassays
Is the Subject Area "Enzyme-linked immunoassays" applicable to this article?

Thanks for your feedback.
Human cytomegalovirus
Is the Subject Area "Human cytomegalovirus" applicable to this article?

Thanks for your feedback.
Binding analysis
Is the Subject Area "Binding analysis" applicable to this article?

Thanks for your feedback.
Cross-linking
Is the Subject Area "Cross-linking" applicable to this article?

Thanks for your feedback.
Cytomegalovirus infection
Is the Subject Area "Cytomegalovirus infection" applicable to this article?

Thanks for your feedback.
Lysine
Is the Subject Area "Lysine" applicable to this article?

Thanks for your feedback.