An siRNA Screen Identifies the U2 snRNP Spliceosome as a Host Restriction Factor for Recombinant Adeno-associated Viruses

Claire A. Schreiber; Toshie Sakuma; Yoshihiro Izumiya; Sara J. Holditch; Raymond D. Hickey; Robert K. Bressin; Upamanyu Basu; Kazunori Koide; Aravind Asokan; Yasuhiro Ikeda

doi:10.1371/journal.ppat.1005082

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Peer-reviewed

Research Article

An siRNA Screen Identifies the U2 snRNP Spliceosome as a Host Restriction Factor for Recombinant Adeno-associated Viruses

Claire A. Schreiber,

Affiliation Department of Molecular Medicine, Mayo Clinic, Rochester, Minnesota, United States of America
⨯
Toshie Sakuma,

Current address: Biological informatics and Experimental Therapeutics, Akita University Graduate School of Medicine, Akita, Japan

Affiliation Department of Molecular Medicine, Mayo Clinic, Rochester, Minnesota, United States of America
⨯
Yoshihiro Izumiya,

Affiliation Department of Dermatology, UC Davis School of Medicine, Sacramento, California, United States of America
⨯
Sara J. Holditch,

Affiliation Department of Molecular Medicine, Mayo Clinic, Rochester, Minnesota, United States of America
⨯
Raymond D. Hickey,

Affiliation Department of Molecular Medicine, Mayo Clinic, Rochester, Minnesota, United States of America
⨯
Robert K. Bressin,

Affiliation Department of Chemistry, University of Pittsburgh, Pittsburgh, Pennsylvania, United States of America
⨯
Upamanyu Basu,

Affiliation Department of Chemistry, University of Pittsburgh, Pittsburgh, Pennsylvania, United States of America
⨯
Kazunori Koide,

Affiliation Department of Chemistry, University of Pittsburgh, Pittsburgh, Pennsylvania, United States of America
⨯
Aravind Asokan,

Affiliation Gene Therapy Center and Department of Genetics, University of North Carolina School of Medicine, Chapel Hill, North Carolina, United States of America
⨯
Yasuhiro Ikeda

* E-mail: ikeda.yasuhiro@mayo.edu

Affiliation Department of Molecular Medicine, Mayo Clinic, Rochester, Minnesota, United States of America
⨯

An siRNA Screen Identifies the U2 snRNP Spliceosome as a Host Restriction Factor for Recombinant Adeno-associated Viruses

Claire A. Schreiber,
Toshie Sakuma,
Yoshihiro Izumiya,
Sara J. Holditch,
Raymond D. Hickey,
Robert K. Bressin,
Upamanyu Basu,
Kazunori Koide,
Aravind Asokan,
Yasuhiro Ikeda

Published: August 5, 2015
https://doi.org/10.1371/journal.ppat.1005082

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