Single Cell Analysis of Lymph Node Tissue from HIV-1 Infected Patients Reveals that the Majority of CD4+ T-cells Contain One HIV-1 DNA Molecule

Lina Josefsson; Sarah Palmer; Nuno R. Faria; Philippe Lemey; Joseph Casazza; David Ambrozak; Mary Kearney; Wei Shao; Shyamasundaran Kottilil; Michael Sneller; John Mellors; John M. Coffin; Frank Maldarelli

doi:10.1371/journal.ppat.1003432

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Open Access

Peer-reviewed

Research Article

Single Cell Analysis of Lymph Node Tissue from HIV-1 Infected Patients Reveals that the Majority of CD4⁺ T-cells Contain One HIV-1 DNA Molecule

Lina Josefsson ,

* E-mail: Lina.josefsson@ki.se

Affiliations Department of Microbiology, Tumor and Cell-biology, Karolinska Institutet, Solna, Sweden, Department of Diagnostics and Vaccinology, Swedish Institute for Communicable Disease Control, Solna, Sweden, HIV Drug Resistance Program, National Cancer Institute, National Institutes of Health, Frederick, Maryland, United States of America
⨯
Sarah Palmer,

Current address: Centre for Virus Research, Westmead Millennium Institute for Medical Research, Sydney, Australia

Affiliations Department of Microbiology, Tumor and Cell-biology, Karolinska Institutet, Solna, Sweden, Department of Diagnostics and Vaccinology, Swedish Institute for Communicable Disease Control, Solna, Sweden, HIV Drug Resistance Program, National Cancer Institute, National Institutes of Health, Frederick, Maryland, United States of America
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Nuno R. Faria,

Affiliation Department of Microbiology and Immunology, Rega Institute, Katholieke Universiteit Leuven, Leuven, Belgium
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Philippe Lemey,

Affiliation Department of Microbiology and Immunology, Rega Institute, Katholieke Universiteit Leuven, Leuven, Belgium
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Joseph Casazza,

Affiliation Vaccine Research Center, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, Maryland, United States of America
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David Ambrozak,

Affiliation Vaccine Research Center, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, Maryland, United States of America
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Mary Kearney,

Affiliation HIV Drug Resistance Program, National Cancer Institute, National Institutes of Health, Frederick, Maryland, United States of America
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Wei Shao,

Affiliation HIV Drug Resistance Program, National Cancer Institute, National Institutes of Health, Frederick, Maryland, United States of America
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Shyamasundaran Kottilil,

Affiliation Laboratory of Immunoregulation, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, Maryland, United States of America
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Michael Sneller,

Affiliation Laboratory of Immunoregulation, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, Maryland, United States of America
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John Mellors,

Affiliation Department of Medicine, Division of Infectious Diseases University of Pittsburgh, Pittsburgh, Pennsylvania, United States of America
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John M. Coffin,

Affiliation Department of Molecular Biology and Microbiology, Tufts University, Boston, Massachusetts, United States of America
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Frank Maldarelli

Affiliation HIV Drug Resistance Program, National Cancer Institute, National Institutes of Health, Frederick, Maryland, United States of America
⨯

Single Cell Analysis of Lymph Node Tissue from HIV-1 Infected Patients Reveals that the Majority of CD4⁺ T-cells Contain One HIV-1 DNA Molecule

Lina Josefsson,
Sarah Palmer,
Nuno R. Faria,
Philippe Lemey,
Joseph Casazza,
David Ambrozak,
Mary Kearney,
Wei Shao,
Shyamasundaran Kottilil,
Michael Sneller

Published: June 20, 2013
https://doi.org/10.1371/journal.ppat.1003432

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