The Interactomes of Influenza Virus NS1 and NS2 Proteins Identify New Host Factors and Provide Insights for ADAR1 Playing a Supportive Role in Virus Replication

Benoît de Chassey; Anne Aublin-Gex; Alessia Ruggieri; Laurène Meyniel-Schicklin; Fabrine Pradezynski; Nathalie Davoust; Thibault Chantier; Lionel Tafforeau; Philippe-Emmanuel Mangeot; Claire Ciancia; Laure Perrin-Cocon; Ralf Bartenschlager; Patrice André; Vincent Lotteau

doi:10.1371/journal.ppat.1003440

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Open Access

Peer-reviewed

Research Article

The Interactomes of Influenza Virus NS1 and NS2 Proteins Identify New Host Factors and Provide Insights for ADAR1 Playing a Supportive Role in Virus Replication

Benoît de Chassey,

Affiliation Hospices Civils de Lyon, Hôpital de la Croix Rousse, Laboratory of Virology, Lyon, France
⨯
Anne Aublin-Gex,

Affiliations CIRI, International Center for Infectiology Research, EVIR Team, Université de Lyon, Lyon, France, Inserm, U1111, Lyon, France, Ecole Normale Supérieure de Lyon, Lyon, France, Université Lyon 1, Centre International de Recherche en Infectiologie, Lyon, France, CNRS, UMR5308, Lyon, France
⨯
Alessia Ruggieri,

Affiliation Department for Infectious Diseases, Molecular Virology, University of Heidelberg, Heidelberg, Germany
⨯
Laurène Meyniel-Schicklin,

Affiliations CIRI, International Center for Infectiology Research, EVIR Team, Université de Lyon, Lyon, France, Inserm, U1111, Lyon, France, Ecole Normale Supérieure de Lyon, Lyon, France, Université Lyon 1, Centre International de Recherche en Infectiologie, Lyon, France, CNRS, UMR5308, Lyon, France
⨯
Fabrine Pradezynski,

Affiliations CIRI, International Center for Infectiology Research, EVIR Team, Université de Lyon, Lyon, France, Inserm, U1111, Lyon, France, Ecole Normale Supérieure de Lyon, Lyon, France, Université Lyon 1, Centre International de Recherche en Infectiologie, Lyon, France, CNRS, UMR5308, Lyon, France
⨯
Nathalie Davoust,

Affiliations CIRI, International Center for Infectiology Research, EVIR Team, Université de Lyon, Lyon, France, Inserm, U1111, Lyon, France, Ecole Normale Supérieure de Lyon, Lyon, France, Université Lyon 1, Centre International de Recherche en Infectiologie, Lyon, France, CNRS, UMR5308, Lyon, France
⨯
Thibault Chantier,

Affiliations CIRI, International Center for Infectiology Research, EVIR Team, Université de Lyon, Lyon, France, Inserm, U1111, Lyon, France, Ecole Normale Supérieure de Lyon, Lyon, France, Université Lyon 1, Centre International de Recherche en Infectiologie, Lyon, France, CNRS, UMR5308, Lyon, France
⨯
Lionel Tafforeau,

Affiliations CIRI, International Center for Infectiology Research, EVIR Team, Université de Lyon, Lyon, France, Inserm, U1111, Lyon, France, Ecole Normale Supérieure de Lyon, Lyon, France, Université Lyon 1, Centre International de Recherche en Infectiologie, Lyon, France, CNRS, UMR5308, Lyon, France
⨯
Philippe-Emmanuel Mangeot,

Affiliations CIRI, International Center for Infectiology Research, EVIR Team, Université de Lyon, Lyon, France, Inserm, U1111, Lyon, France, Ecole Normale Supérieure de Lyon, Lyon, France, Université Lyon 1, Centre International de Recherche en Infectiologie, Lyon, France, CNRS, UMR5308, Lyon, France
⨯
Claire Ciancia,

Affiliations CIRI, International Center for Infectiology Research, EVIR Team, Université de Lyon, Lyon, France, Inserm, U1111, Lyon, France, Ecole Normale Supérieure de Lyon, Lyon, France, Université Lyon 1, Centre International de Recherche en Infectiologie, Lyon, France, CNRS, UMR5308, Lyon, France
⨯
Laure Perrin-Cocon,

Affiliations CIRI, International Center for Infectiology Research, EVIR Team, Université de Lyon, Lyon, France, Inserm, U1111, Lyon, France, Ecole Normale Supérieure de Lyon, Lyon, France, Université Lyon 1, Centre International de Recherche en Infectiologie, Lyon, France, CNRS, UMR5308, Lyon, France
⨯
Ralf Bartenschlager,

Affiliation Department for Infectious Diseases, Molecular Virology, University of Heidelberg, Heidelberg, Germany
⨯
Patrice André,

Affiliations Hospices Civils de Lyon, Hôpital de la Croix Rousse, Laboratory of Virology, Lyon, France, CIRI, International Center for Infectiology Research, EVIR Team, Université de Lyon, Lyon, France, Inserm, U1111, Lyon, France, Ecole Normale Supérieure de Lyon, Lyon, France, Université Lyon 1, Centre International de Recherche en Infectiologie, Lyon, France, CNRS, UMR5308, Lyon, France
⨯
Vincent Lotteau

* E-mail: vincent.lotteau@inserm.fr

Affiliations CIRI, International Center for Infectiology Research, EVIR Team, Université de Lyon, Lyon, France, Inserm, U1111, Lyon, France, Ecole Normale Supérieure de Lyon, Lyon, France, Université Lyon 1, Centre International de Recherche en Infectiologie, Lyon, France, CNRS, UMR5308, Lyon, France
⨯

The Interactomes of Influenza Virus NS1 and NS2 Proteins Identify New Host Factors and Provide Insights for ADAR1 Playing a Supportive Role in Virus Replication

Benoît de Chassey,
Anne Aublin-Gex,
Alessia Ruggieri,
Laurène Meyniel-Schicklin,
Fabrine Pradezynski,
Nathalie Davoust,
Thibault Chantier,
Lionel Tafforeau,
Philippe-Emmanuel Mangeot,
Claire Ciancia

Published: July 4, 2013
https://doi.org/10.1371/journal.ppat.1003440

About the Authors

Benoît de Chassey

Affiliation Hospices Civils de Lyon, Hôpital de la Croix Rousse, Laboratory of Virology, Lyon, France

Anne Aublin-Gex

Affiliations CIRI, International Center for Infectiology Research, EVIR Team, Université de Lyon, Lyon, France, Inserm, U1111, Lyon, France, Ecole Normale Supérieure de Lyon, Lyon, France, Université Lyon 1, Centre International de Recherche en Infectiologie, Lyon, France, CNRS, UMR5308, Lyon, France

Alessia Ruggieri

Affiliation Department for Infectious Diseases, Molecular Virology, University of Heidelberg, Heidelberg, Germany

Laurène Meyniel-Schicklin

Fabrine Pradezynski

Nathalie Davoust

Thibault Chantier

Lionel Tafforeau

Philippe-Emmanuel Mangeot

Claire Ciancia

Laure Perrin-Cocon

Ralf Bartenschlager

Affiliation Department for Infectious Diseases, Molecular Virology, University of Heidelberg, Heidelberg, Germany

Patrice André

Affiliations Hospices Civils de Lyon, Hôpital de la Croix Rousse, Laboratory of Virology, Lyon, France, CIRI, International Center for Infectiology Research, EVIR Team, Université de Lyon, Lyon, France, Inserm, U1111, Lyon, France, Ecole Normale Supérieure de Lyon, Lyon, France, Université Lyon 1, Centre International de Recherche en Infectiologie, Lyon, France, CNRS, UMR5308, Lyon, France

Vincent Lotteau

* E-mail: vincent.lotteau@inserm.fr

Competing Interests

The authors have declared that no competing interests exist.

Author Contributions

Conceived and designed the experiments: BdC PA VL. Performed the experiments: BdC AAG AR FP ND TC LMS LT PEM CC LPC. Analyzed the data: BdC VL PA LMS. Contributed reagents/materials/analysis tools: RB. Wrote the paper: BdC VL PA RB.

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Viral replication
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Influenza viruses
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Dengue virus
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Small interfering RNA
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Influenza A virus
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Transfection
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Protein interactions
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Interferons
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