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Site-directed mutagenesis

Enhanced cadmium binding ability in response to novel modifications in a Paramecium cadmium metallothionein PMCd1

Hira Nizam, Asmara Imtiaz, [ ... ], Abdul Rauf Shakoori

Larger active site in an ancestral hydroxynitrile lyase increases catalytically promiscuous esterase activity

Bryan J. Jones, Robert L. Evans III, [ ... ], Romas J. Kazlauskas

Exploring the molecular structures that confer ligand selectivity for galanin type II and III receptors

Yoo-Na Lee, Arfaxad Reyes-Alcaraz, [ ... ], Jae Young Seong

Transcriptional regulation of the Pseudomonas aeruginosa iron-sulfur cluster assembly pathway by binding of IscR to multiple sites

Kritsakorn Saninjuk, Adisak Romsang, [ ... ], Skorn Mongkolsuk

Peak emission wavelength and fluorescence lifetime are coupled in far-red, GFP-like fluorescent proteins

Laura Canty, Santosh Hariharan, [ ... ], David W. Andrews

Molecular basis of inhibition of acid sensing ion channel 1A by diminazene

Aram J. Krauson, James G. Rooney, Marcelo D. Carattino

Generation of efficient mutants of endoglycosidase from Streptococcus pyogenes and their application in a novel one-pot transglycosylation reaction for antibody modification

Mitsuhiro Iwamoto, Yukiko Sekiguchi, [ ... ], Jun Hasegawa

Three unrelated and unexpected amino acids determine the susceptibility of the interface cysteine to a sulfhydryl reagent in the triosephosphate isomerases of two trypanosomes

Selma Díaz-Mazariegos, Nallely Cabrera, Ruy Perez-Montfort

An L213A variant of β-glycosidase from Sulfolobus solfataricus with increased α-L-arabinofuranosidase activity converts ginsenoside Rc to compound K

Ji-Hyeon Choi, Kyung-Chul Shin, Deok-Kun Oh

Exploring the role of L209 residue in the active site of NDM-1 a metallo-β-lactamase

Francesca Marcoccia, Hanna-Kirsti S. Leiros, [ ... ], Mariagrazia Perilli

Evolution-guided adaptation of an adenylation domain substrate specificity to an unusual amino acid

Simon Vobruba, Stanislav Kadlcik, Radek Gazak, Jiri Janata

Engineering substrate promiscuity in halophilic alcohol dehydrogenase (HvADH2) by in silico design

Jennifer Cassidy, Larah Bruen, [ ... ], Francesca Paradisi

Involvement of arginine 878 together with Ca²⁺ in mouse aminopeptidase A substrate specificity for N-terminal acidic amino-acid residues

Pierre Couvineau, Hugo de Almeida, [ ... ], Xavier Iturrioz

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