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Sequencing techniques

Gene Mapping via Bulked Segregant RNA-Seq (BSR-Seq)

Sanzhen Liu, Cheng-Ting Yeh, [ ... ], Patrick S. Schnable

Evaluating Methods for Isolating Total RNA and Predicting the Success of Sequencing Phylogenetically Diverse Plant Transcriptomes

Marc T. J. Johnson, Eric J. Carpenter, [ ... ], Gane Ka-Shu Wong

Expanding the Diversity of Mycobacteriophages: Insights into Genome Architecture and Evolution

Welkin H. Pope, Deborah Jacobs-Sera, [ ... ], Graham F. Hatfull

Fast Computation and Applications of Genome Mappability

Thomas Derrien, Jordi Estellé, [ ... ], Paolo Ribeca

Inexpensive Multiplexed Library Preparation for Megabase-Sized Genomes

Michael Baym, Sergey Kryazhimskiy, [ ... ], Roy Kishony

An Integrated Pipeline for de Novo Assembly of Microbial Genomes

Andrew Tritt, Jonathan A. Eisen, Marc T. Facciotti, Aaron E. Darling

Microbiome Profiling by Illumina Sequencing of Combinatorial Sequence-Tagged PCR Products

Gregory B. Gloor, Ruben Hummelen, [ ... ], Gregor Reid

An Improved Genotyping by Sequencing (GBS) Approach Offering Increased Versatility and Efficiency of SNP Discovery and Genotyping

Humira Sonah, Maxime Bastien, [ ... ], François Belzile

Genome-Wide Identification of Transcription Start Sites, Promoters and Transcription Factor Binding Sites in E. coli

Alfredo Mendoza-Vargas, Leticia Olvera, [ ... ], Enrique Morett

A workflow with R: Phylogenetic analyses and visualizations using mitochondrial cytochrome b gene sequences

Emine Toparslan, Kemal Karabag, Ugur Bilge

High-Throughput Sequencing of Arabidopsis microRNAs: Evidence for Frequent Birth and Death of MIRNA Genes

Noah Fahlgren, Miya D. Howell, [ ... ], James C. Carrington

CCTop: An Intuitive, Flexible and Reliable CRISPR/Cas9 Target Prediction Tool

Manuel Stemmer, Thomas Thumberger, [ ... ], Juan L. Mateo

sgRNAcas9: A Software Package for Designing CRISPR sgRNA and Evaluating Potential Off-Target Cleavage Sites

Shengsong Xie, Bin Shen, [ ... ], Yonglian Zhang

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