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Sequencing techniques

Differentially expressed transcript isoforms associate with resistance to tuberculin skin test and interferon gamma release assay conversion

Jason D. Simmons, R. Max Segnitz, Kimberly A. Dill-McFarland, Catherine M. Stein, Glenna J. Peterson, Harriet Mayanja-Kizza, W. Henry Boom, Thomas R. Hawn

published 14 Apr 2023

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A comprehensive performance analysis of sequence-based within-sample testing NIPT methods

Tom Mokveld, Zaid Al-Ars, Erik A. Sistermans, Marcel Reinders

published 14 Apr 2023

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Optimised protocol for monitoring SARS-CoV-2 in wastewater using reverse complement PCR-based whole-genome sequencing

Harry T. Child, Paul A. O’Neill, Karen Moore, William Rowe, Hubert Denise, David Bass, Matthew J. Wade, Matt Loose, Steve Paterson, Ronny van Aerle, Aaron R. Jeffries

published 14 Apr 2023

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Direct and culture-enriched 16S rRNA sequencing of cecal content of healthy horses and horses with typhlocolitis

Luiza S. Zakia, Diego E. Gomez, Benjamin B. Caddey, Patrick Boerlin, Michael G. Surette, Luis G. Arroyo

published 13 Apr 2023

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Designed and validated novel allele-specific primer to differentiate Kernel Row Number (KRN) in tropical field corn

Ganapati Mukri, Kumari Shilpa, R. N. Gadag, Jayant S. Bhat, Chandu Singh, Navin C. Gupta, Chandra Prabha, Sahana Police Patil

published 12 Apr 2023

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CRISPR/Cas9-Mediated genomic knock out of tyrosine hydroxylase and yellow genes in cricket Gryllus bimaculatus

Yun Bai, Yuan He, Chu-Ze Shen, Kai Li, Dong-Liang Li, Zhu-Qing He

published 10 Apr 2023

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Immunoglobulin profiling with large high-density peptide microarrays as screening method to detect candidate proteins for future biomarker detection in dogs with steroid-responsive meningitis-arteritis

Jasmin Nicole Nessler, Andrea Tipold

published 10 Apr 2023

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The selection of software and database for metagenomics sequence analysis impacts the outcome of microbial profiling and pathogen detection

Ruijie Xu, Sreekumari Rajeev, Liliana C. M. Salvador

published 07 Apr 2023

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A Hidradenitis Suppurativa molecular disease signature derived from patient samples by high-throughput RNA sequencing and re-analysis of previously reported transcriptomic data sets

Johannes M. Freudenberg, Zhi Liu, Jennifer Singh, Elizabeth Thomas, Christopher Traini, Deepak K. Rajpal, Christopher J. Sayed

published 06 Apr 2023

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Optimization of gene editing in cowpea through protoplast transformation and agroinfiltration by targeting the phytoene desaturase gene

Aya Bridgeland, Sudip Biswas, Nikolaos Tsakirpaloglou, Michael J. Thomson, Endang M. Septiningsih

published 05 Apr 2023

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Circulating miR-20b-5p and miR-330-3p are novel biomarkers for progression of atrial fibrillation: Intracardiac/extracardiac plasma sample analysis by small RNA sequencing

Masahide Harada, Daisuke Okuzaki, Akemi Yamauchi, Shiho Ishikawa, Yoshihiro Nomura, Asuka Nishimura, Yuji Motoike, Masayuki Koshikawa, Keisuke Hitachi, Kunihiro Tsuchida, Kentaro Amano, Atsuo Maekawa, Yasushi Takagi, Eiichi Watanabe, Yukio Ozaki, Hideo Izawa

published 04 Apr 2023

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RNA sequencing revealed the multi-stage transcriptome transformations during the development of gallbladder cancer associated with chronic inflammation

Sen Yang, Litao Qin, Pan Wu, Yanbing Liu, Yanling Zhang, Bing Mao, Yiyang Yan, Shuai Yan, Feilong Tan, Xueliang Yue, Hongshan Liu, Huanzhou Xue

published 30 Mar 2023

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Rapid genomic surveillance of SARS-CoV-2 in a dense urban community of Kathmandu Valley using sewage samples

Rajindra Napit, Prajwol Manandhar, Ashok Chaudhary, Bishwo Shrestha, Ajit Poudel, Roji Raut, Saman Pradhan, Samita Raut, Pragun G. Rajbhandari, Anupama Gurung, Rajesh M. Rajbhandari, Sameer M. Dixit, Jessica S. Schwind, Christine K. Johnson, Jonna K. Mazet, Dibesh B. Karmacharya

published 30 Mar 2023

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