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Sequencing techniques

Reducing the Effects of PCR Amplification and Sequencing Artifacts on 16S rRNA-Based Studies

Patrick D. Schloss, Dirk Gevers, Sarah L. Westcott

Comparing bioinformatic pipelines for microbial 16S rRNA amplicon sequencing

Andrei Prodan, Valentina Tremaroli, [ ... ], Evgeni Levin

Searching for Tissue-Specific Expression Pattern-Linked Nucleotides of UGT1A Isoforms

Wei Zhang, Wanqing Liu, Federico Innocenti, Mark J. Ratain

Examining Sources of Error in PCR by Single-Molecule Sequencing

Vladimir Potapov, Jennifer L. Ong

Strengths and Limitations of 16S rRNA Gene Amplicon Sequencing in Revealing Temporal Microbial Community Dynamics

Rachel Poretsky, Luis M. Rodriguez-R, [ ... ], Konstantinos T. Konstantinidis

A Comprehensive Evaluation of PCR Primers to Amplify the nifH Gene of Nitrogenase

John Christian Gaby, Daniel H. Buckley

Analytical and Clinical Validation of a Digital Sequencing Panel for Quantitative, Highly Accurate Evaluation of Cell-Free Circulating Tumor DNA

Richard B. Lanman, Stefanie A. Mortimer, [ ... ], AmirAli Talasaz

Whole-Genome Sequencing of the World’s Oldest People

Hinco J. Gierman, Kristen Fortney, [ ... ], Stuart K. Kim

Mind the Gap: Upgrading Genomes with Pacific Biosciences RS Long-Read Sequencing Technology

Adam C. English, Stephen Richards, [ ... ], Richard A. Gibbs

Development of a Prokaryotic Universal Primer for Simultaneous Analysis of Bacteria and Archaea Using Next-Generation Sequencing

Shunsuke Takahashi, Junko Tomita, [ ... ], Miyuki Nishijima

Prospective Genomic Characterization of the German Enterohemorrhagic Escherichia coli O104:H4 Outbreak by Rapid Next Generation Sequencing Technology

Alexander Mellmann, Dag Harmsen, [ ... ], Helge Karch

Sequencing DNA with nanopores: Troubles and biases

Clara Delahaye, Jacques Nicolas

SNP Calling, Genotype Calling, and Sample Allele Frequency Estimation from New-Generation Sequencing Data

Rasmus Nielsen, Thorfinn Korneliussen, [ ... ], Jun Wang

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