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Sequencing techniques

Microsatellite development for Theridion evexum (Araneae: Theridiidae) using low-coverage genome sequencing and the MiMi script

Ruth Madrigal-Brenes, Gilbert Barrantes, Luis Sandoval, Eric J. Fuchs

published 15 Sep 2025

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Whole blood RNA profiling in cats dissects the host immunological response during recovery from feline infectious peritonitis

Katharina Zwicklbauer, Pilar Grassl, Martin Alberer, Laura Kolberg, Nina A. Schweintzger, Sonja Härtle, Kaspar Matiasek, Regina Hofmann-Lehmann, Katrin Hartmann, Caroline C. Friedel, Ulrich von Both

published 12 Sep 2025

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Gene expression alterations in testicular biopsies from males with spermatogenesis arrest identified by transcriptome analysis

Osamah Batiha, Esra’a Al-Zoubi, Rowida Almomani, Mohammad A. Al Smadi, Sura Alrawabdeh, Omar Alshokaibi, Hussam Abu-Farsakh, Abedalrhman Alkhateeb, Masood Abu-Halima

published 12 Sep 2025

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Evaluation of the Xpert MTB/XDR test for detection of isoniazid, fluoroquinolones, and second-line injectable drugs resistance to Mycobacterium tuberculosis—Anhui Province, China

Ruiqing Zhang, Xundi Bao, Fangjin Bao, Chong Teng, Dongfang Xu, Zhou Liu, Yue Li, Bing Zhao, Hui Xia, Ruida Xing, Xichao Ou, Yanlin Zhao

published 12 Sep 2025

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Analytical validation (accuracy, reproducibility, limit of detection) and gene expression analysis of FoundationOneRNA assay for fusion detection in 189 clinical tumor specimens

Daokun Sun, Richard S. P. Huang, Michelle Green, Chenming Cui, Saumya D. Sisoudiya, Andrej Savol, Chang Xu, Cui Guo, Joel Skoletsky, Justin M. Allen, Khaled Tolba, Varun Pattani, Alyssa Tarzia, Jennifer Whiting, Yanhua Tang, Lee A. Albacker, Christine Vietz, Michael Datto, Jie He, William Richard Jeck

published 12 Sep 2025

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Potential role of miR-29b from mesenchymal stromal cell-derived extracellular vesicles in leukemic cell progression

Heejei Yoon, Silvia Park, Yong-Rim Kwon, Yoo-Jin Kim

published 10 Sep 2025

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An improved nuclei isolation protocol from leaf tissue for single-cell transcriptomics

Gabriela Madrid, Gabriel Angelo Saraiva Raimundo, Fabian Andres Reyes, Edgard Augusto de Toledo Picoli, Marcio F. R. Resende Jr., Kelly M. Balmant

published 10 Sep 2025

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Unveiling the mechanism of Pogostemon cablin (Blanco) Benth in treating heat illnesses via network pharmacology and molecular simulation

Xiang Li, Sishi Jiang, Haili Li, Shilin Li, Yingchun Hu

published 08 Sep 2025

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Exploring extreme environments in Türkiye for novel P450s through metagenomic analysis

Hande Mumcu, Julian Zaugg, Irem Keles, Aycan Kayrav, Nurgul Balci, David R. Nelson, Philip Hugenholtz, Elizabeth M. J. Gillam, Nevin Gul Karaguler

published 08 Sep 2025

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A novel mitovirus associated with the fungal entomopathogen Zoophthora radicans

Meaghan J. Adler, McKayla M. Martin, Paula Rozo-Lopez, Benjamin J. Parker

published 05 Sep 2025

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Transcriptomic changes associated with infection of Nicotiana benthamiana plants with tomato ringspot virus (genus Nepovirus) during the acute symptomatic stage and after symptom recovery

Dinesh Babu Paudel, Ana Priscilla Montenegro Alonso, Joan Chisholm, Huogen Xiao, Hélène Sanfaçon

published 02 Sep 2025

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Identification of BOLD engine deficiencies and suggestions for improvement based on a curated Tachina (Diptera) record set

Frederik Stein, Oliver Gailing

published 29 Aug 2025

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Modular and cloud-based bioinformatics pipelines for high-confidence biomarker detection in cancer immunotherapy clinical trials

Cu Nguyen, Trinh Nguyen, Gloria Trivitt, Brian Capaldo, Chunhua Yan, Qingrong Chen, Nicholas Renzette, Umit Topaloglu, Daoud Meerzaman

published 26 Aug 2025

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