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Selectable markers

Direct in situ protein tagging in Chlamydomonas reinhardtii utilizing TIM, a method for CRISPR/Cas9-based targeted insertional mutagenesis

Yuqing Hou, Xi Cheng, George B. Witman

published 09 Dec 2022

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Single- and duplex TaqMan-quantitative PCR for determining the copy numbers of integrated selection markers during site-specific mutagenesis in Toxoplasma gondii by CRISPR-Cas9

Kai Pascal Alexander Hänggeli, Andrew Hemphill, Norbert Müller, Bernd Schimanski, Philipp Olias, Joachim Müller, Ghalia Boubaker

published 16 Sep 2022

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Introduction of a leaky stop codon as molecular tool in Chlamydomonas reinhardtii

Oliver D. Caspari

published 20 Aug 2020

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A new toolkit for gene tagging in Candida albicans containing recyclable markers

Encarnación Dueñas-Santero, Ana Santos-Almeida, Patricia Rojo-Dominguez, Francisco del Rey, Jaime Correa-Bordes, Carlos R. Vázquez de Aldana

published 11 Jul 2019

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Optimization of T-DNA architecture for Cas9-mediated mutagenesis in Arabidopsis

Baptiste Castel, Laurence Tomlinson, Federica Locci, Ying Yang, Jonathan D. G. Jones

published 09 Jan 2019

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An intragenic approach to confer glyphosate resistance in chile (Capsicum annuum) by introducing an in vitro mutagenized chile EPSPS gene encoding for a glyphosate resistant EPSPS protein

Jose Luis Ortega, Wathsala Rajapakse, Suman Bagga, Kimberly Apodaca, Yvonne Lucero, Champa Sengupta-Gopalan

published 12 Apr 2018

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A method for labeling proteins with tags at the native genomic loci in budding yeast

Qian Wang, Huijun Xue, Siqi Li, Ying Chen, Xuelei Tian, Xin Xu, Wei Xiao, Yu Vincent Fu

published 01 May 2017

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Rapid Generation of Marker-Free P. falciparum Fluorescent Reporter Lines Using Modified CRISPR/Cas9 Constructs and Selection Protocol

Catherin Marin Mogollon, Fiona J. A. van Pul, Takashi Imai, Jai Ramesar, Séverine Chevalley-Maurel, Guido M. de Roo, Sabrina A. J. Veld, Hans Kroeze, Blandine M. D. Franke-Fayard, Chris J. Janse, Shahid M. Khan

published 20 Dec 2016

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A SIX1 Homolog in Fusarium oxysporum f. sp. conglutinans Is Required for Full Virulence on Cabbage

Erfeng Li, Gang Wang, Jiling Xiao, Jian Ling, Yuhong Yang, Bingyan Xie

published 24 Mar 2016

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Genetic Evidence for the Role of the Vacuole in Supplying Secretory Organelles with Ca²⁺ in Hansenula polymorpha

Anastasia V. Fokina, Maria B. Chechenova, Azamat V. Karginov, Michael D. Ter-Avanesyan, Michael O. Agaphonov

published 30 Dec 2015

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Development of the First Cisgenic Apple with Increased Resistance to Fire Blight

Thomas D. Kost, Cesare Gessler, Melanie Jänsch, Henryk Flachowsky, Andrea Patocchi, Giovanni A. L. Broggini

published 01 Dec 2015

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Development of Selectable Marker-Free Transgenic Rice Plants with Enhanced Seed Tocopherol Content through FLP/FRT-Mediated Spontaneous Auto-Excision

Hee-Jong Woo, Yang Qin, Soo-Yun Park, Soon Ki Park, Yong-Gu Cho, Kong-Sik Shin, Myung-Ho Lim, Hyun-Suk Cho

published 14 Jul 2015

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A Genomic Safe Haven for Mutant Complementation in Cryptococcus neoformans

Samantha D. M. Arras, Jessica L. Chitty, Kirsten L. Blake, Benjamin L. Schulz, James A. Fraser

published 09 Apr 2015

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