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Selectable markers

Direct in situ protein tagging in Chlamydomonas reinhardtii utilizing TIM, a method for CRISPR/Cas9-based targeted insertional mutagenesis

Yuqing Hou, Xi Cheng, George B. Witman

Single- and duplex TaqMan-quantitative PCR for determining the copy numbers of integrated selection markers during site-specific mutagenesis in Toxoplasma gondii by CRISPR-Cas9

Kai Pascal Alexander Hänggeli, Andrew Hemphill, [ ... ], Ghalia Boubaker

Introduction of a leaky stop codon as molecular tool in Chlamydomonas reinhardtii

Oliver D. Caspari

A new toolkit for gene tagging in Candida albicans containing recyclable markers

Encarnación Dueñas-Santero, Ana Santos-Almeida, [ ... ], Carlos R. Vázquez de Aldana

Optimization of T-DNA architecture for Cas9-mediated mutagenesis in Arabidopsis

Baptiste Castel, Laurence Tomlinson, [ ... ], Jonathan D. G. Jones

An intragenic approach to confer glyphosate resistance in chile (Capsicum annuum) by introducing an in vitro mutagenized chile EPSPS gene encoding for a glyphosate resistant EPSPS protein

Jose Luis Ortega, Wathsala Rajapakse, [ ... ], Champa Sengupta-Gopalan

A method for labeling proteins with tags at the native genomic loci in budding yeast

Qian Wang, Huijun Xue, [ ... ], Yu Vincent Fu

Rapid Generation of Marker-Free P. falciparum Fluorescent Reporter Lines Using Modified CRISPR/Cas9 Constructs and Selection Protocol

Catherin Marin Mogollon, Fiona J. A. van Pul, [ ... ], Shahid M. Khan

A SIX1 Homolog in Fusarium oxysporum f. sp. conglutinans Is Required for Full Virulence on Cabbage

Erfeng Li, Gang Wang, [ ... ], Bingyan Xie

Genetic Evidence for the Role of the Vacuole in Supplying Secretory Organelles with Ca²⁺ in Hansenula polymorpha

Anastasia V. Fokina, Maria B. Chechenova, [ ... ], Michael O. Agaphonov

Development of the First Cisgenic Apple with Increased Resistance to Fire Blight

Thomas D. Kost, Cesare Gessler, [ ... ], Giovanni A. L. Broggini

Development of Selectable Marker-Free Transgenic Rice Plants with Enhanced Seed Tocopherol Content through FLP/FRT-Mediated Spontaneous Auto-Excision

Hee-Jong Woo, Yang Qin, [ ... ], Hyun-Suk Cho

A Genomic Safe Haven for Mutant Complementation in Cryptococcus neoformans

Samantha D. M. Arras, Jessica L. Chitty, [ ... ], James A. Fraser

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