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Ribonucleoproteins

Evaluation of subretinally delivered Cas9 ribonucleoproteins in murine and porcine animal models highlights key considerations for therapeutic translation of genetic medicines

Spencer C. Wei, Aaron J. Cantor, [ ... ], Adam P. Silverman

A simple validation and screening method for CRISPR/Cas9-mediated gene editing in mouse embryos to facilitate genetically modified mice production

Dawid Winiarczyk, Hossein Khodadadi, Paweł Leszczyński, Hiroaki Taniguchi

Systematic identification and characterization of high efficiency Cas9 guide RNAs for therapeutic targeting of ADAR

Benjamin G. Gowen, Kory Melton, [ ... ], Spencer C. Wei

Development of an optimized protocol for generating knockout cancer cell lines using the CRISPR/Cas9 system, with emphasis on transient transfection

Seyed Alireza Mousavi Kahaki, Nayereh Ebrahimzadeh, Hossein Fahimi, Arfa Moshiri

Mechanisms of active diffusion of vesicular stomatitis virus inclusion bodies and cellular early endosomes in the cytoplasm of mammalian cells

Steven J. Moran, Ryan Oglietti, [ ... ], Douglas S. Lyles

Gene knock-outs in human CD34+ hematopoietic stem and progenitor cells and in the human immune system of mice

Daniel A. Kuppers, Jonathan Linton, [ ... ], Patrick J. Paddison

A precise gene delivery approach for human induced pluripotent stem cells using Cas9 RNP complex and recombinant AAV6 donor vectors

Koollawat Chupradit, Nontaphat Thongsin, Chatchai Tayapiwatana, Methichit Wattanapanitch

Investigating CRISPR/Cas9 gene drive for production of disease-preventing prion gene alleles

Andrew R. Castle, Serene Wohlgemuth, Luis Arce, David Westaway

Genome editing using preassembled CRISPR-Cas9 ribonucleoprotein complexes in Fusarium graminearum

Nahyun Lee, Jiyeun Park, [ ... ], Hokyoung Son

Protocol for assessment of the efficiency of CRISPR/Cas RNP delivery to different types of target cells

Marina A. Tyumentseva, Aleksandr I. Tyumentsev, Vasiliy G. Akimkin

Pursuit of chlorovirus genetic transformation and CRISPR/Cas9-mediated gene editing

Eric A. Noel, Donald P. Weeks, James L. Van Etten

Markers of acute toxicity of DDT exposure in pancreatic beta-cells determined by a proteomic approach

Nela Pavlikova, Jan Sramek, [ ... ], Jan Kovar

Interaction between influenza A virus nucleoprotein and PB2 cap-binding domain is mediated by RNA

Wun-Chung Szeto, Ho-Pan Hsia, Yun-Sang Tang, Pang-Chui Shaw

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