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Reverse genetics

A mix-and-match reverse genetics system for evaluating genetic determinants of orthobunyavirus neurological disease

Heini M. Miettinen, Matthew J. Abbott, Alyssa B. Evans

published 28 Apr 2025

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In vitro one-pot construction of influenza viral genomes for virus particle synthesis based on reverse genetics system

Ryota Tanaka, Kenji Tamao, Mana Ono, Seiya Yamayoshi, Yoshihiro Kawaoka, Masayuki Su’etsugu, Hiroyuki Noji, Kazuhito V. Tabata

published 08 Nov 2024

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One-pot Golden Gate Assembly of an avian infectious bronchitis virus reverse genetics system

Katharina Bilotti, Sarah Keep, Andrew P. Sikkema, John M. Pryor, James Kirk, Katalin Foldes, Nicole Doyle, Ge Wu, Graham Freimanis, Giulia Dowgier, Oluwapelumi Adeyemi, S. Kasra Tabatabaei, Gregory J. S. Lohman, Erica Bickerton

published 25 Jul 2024

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Growth enhancement of porcine epidemic diarrhea virus (PEDV) in Vero E6 cells expressing PEDV nucleocapsid protein

Benjamas Liwnaree, Jaraspim Narkpuk, Suttipun Sungsuwan, Anan Jongkaewwattana, Peera Jaru-Ampornpan

published 06 Mar 2019

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Haiku: New paradigm for the reverse genetics of emerging RNA viruses

Thérèse Atieh, Miriam Diala El Ayoubi, Fabien Aubry, Stéphane Priet, Xavier de Lamballerie, Antoine Nougairède

published 13 Feb 2018

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Distinct virulence of Rift Valley fever phlebovirus strains from different genetic lineages in a mouse model

Tetsuro Ikegami, Aaron Balogh, Shoko Nishiyama, Nandadeva Lokugamage, Tais B. Saito, John C. Morrill, Vinay Shivanna, Sabarish V. Indran, Lihong Zhang, Jennifer K. Smith, David Perez, Terry L. Juelich, Igor Morozov, William C. Wilson, Alexander N. Freiberg, Juergen A. Richt

published 21 Dec 2017

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The Ability of a Non-Egg Adapted (Cell-Like) A(H1N1)pdm09 Virus to Egg-Adapt at HA Loci Other than 222 and 223 and Its Effect on the Yield of Viral Protein

Carolyn Nicolson, Ruth Harvey, Othmar G. Engelhardt, James S. Robertson

published 18 Nov 2016

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Molecular Basis for Antigenic Diversity of Genus Betanodavirus

Valentina Panzarin, Anna Toffan, Miriam Abbadi, Alessandra Buratin, Marzia Mancin, Stine Braaen, Christel Moræus Olsen, Luca Bargelloni, Espen Rimstad, Giovanni Cattoli

published 20 Jul 2016

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Development of a PCR-Based Reverse Genetics System for an Attenuated Duck Tembusu Virus Strain

Xiaogang Wu, Ying Shi, Dawei Yan, Xuesong Li, Pixi Yan, Xuyuan Gao, Yuee Zhang, Lei Yu, Chaochao Ren, Guoxin Li, Liping Yan, Qiaoyang Teng, Zejun Li

published 01 Jun 2016

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Chimeric NP Non Coding Regions between Type A and C Influenza Viruses Reveal Their Role in Translation Regulation

Bernadette Crescenzo-Chaigne, Cyril Barbezange, Vianney Frigard, Damien Poulain, Sylvie van der Werf

published 30 Sep 2014

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Comparative Analysis of Seven Viral Nuclear Export Signals (NESs) Reveals the Crucial Role of Nuclear Export Mediated by the Third NES Consensus Sequence of Nucleoprotein (NP) in Influenza A Virus Replication

Nopporn Chutiwitoonchai, Michinori Kakisaka, Kazunori Yamada, Yoko Aida

published 13 Aug 2014

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The Breadth of Cross Sub-Type Neutralisation Activity of a Single Domain Antibody to Influenza Hemagglutinin Can Be Increased by Antibody Valency

Simon E. Hufton, Paul Risley, Christina R. Ball, Diane Major, Othmar G. Engelhardt, Stephen Poole

published 01 Aug 2014

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RNA Elements in Open Reading Frames of the Bluetongue Virus Genome Are Essential for Virus Replication

Femke Feenstra, René G. P. van Gennip, Sandra G. P. van de Water, Piet A. van Rijn

published 21 Mar 2014

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