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Molecular biology techniques

Bidirectional phenotypic transition in macrophages influences triple-negative breast cancer cell immune evasive patterns and response to chemotherapies in a co-culture spheroid model

Chen Cheng, Brett A. McGregor, Junguk Hur, Colin K. Combs

published 14 Aug 2026

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Good DNA- and sufficient RNA integrity in formalin-free paraffin-embedded tissues

Maarten Niemantsverdriet, Natalie Methorst, Sanne Yvet Hoek, Ageeth Knol, Agnes Marije Hoogland

published 14 Aug 2026

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MyD88 restricts dysbiosis-mediated inflammation in filaggrin deficient skin

Meng-Jen Wu, Advaitaa Ravipati, Yu Wang, Laine E. Feller, Alyssa Chu, Rishi Damarla, Zhiyang Li, Lam C. Tsoi, Ryleigh Griffin, Peng Hou, Hai Liang, Raif Geha, Julia A. Segre, Heidi H. Kong, Johann E. Gudjonsson, Nathan K. Archer

published 14 Aug 2026

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Development of KASP molecular markers and construction of fingerprinting for cowpea (Vigna unguiculata (L.) Walp.) based on ddRAD-Seq

Juan Xiang, Zhuoling Zhong, Chengming Zhang, Min He, Kun Cai, Lanping Gu, Li Xu, Shilin Su, Yi Zou, Jie Li, Kehao Cui, Huimin Qiu, Bengang Xian, Shaohong Fu, Ling Chen, Xiaowei Liu

published 14 Aug 2026

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Study protocol: Effect of inulin supplementation on gut microbiota in patients with oral lichen planus — A double-blind, randomised, placebo-controlled parallel-group trial

Koki Nambu, Naoki Kaneko, Shiho Yokomizo, Hu Chen, Lijing Yan, Wang Shiyao, Ryusei Shizuma, Eiji Iwata, Yukiko Ohyama, Shohei Akagawa, Soichiro Ibaragi, Shintaro Kawano, Masafumi Moriyama

published 13 Aug 2026

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Regulatory effects of smoking cessation on the cellular microenvironment and differentially expressed genes in precancerous lesions of pulmonary nodules in mice based on single-cell RNA sequencing and immune repertoire-sequencing

Xintong Wang, Fang Tang, Jiayu Qin, Tiquan Xiao, Liwei Shi, Shujun Zhang, Chunli Che

published 13 Aug 2026

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CLR-Seq: A pipeline to identify bacterial microbiota species with immune-relevant glycan moieties through human C-type lectin receptor interaction

Jasper Mol, Rob van Dalen, Yvonne Pannekoek, Malgorzata E. Mnich, Marcel R. de Zoete, Mark Davids, Hilde Herrema, Nina M. van Sorge

published 13 Aug 2026

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L-Phenylalanine restriction enhances L-boronophenylalanine uptake and improves boron neutron capture therapy efficacy in tumor cell lines

Yuki Tamari, Rie Saba, Takushi Takata, Hiroyuki Kimura, Mao Takita, Kenta Yashiro, Minoru Suzuki, Hideya Yamazaki, Kei Yamada

published 13 Aug 2026

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Targeted sequencing provides genetic insights into familial cardiovascular disease among young adults in a high consanguinity population

Nauman Arif, Imran Khan, Taj Ali Khan, Sajjad Ahmad, Fatima Nauman, Gulzar Ahmad, Muhammad Ijaz, Bilal Ahmad, Syed Munawar Hassan, Saima Afaq, Zia Ul Haq

published 13 Aug 2026

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Shedding light on neural learning to rank models for anticancer drug prioritization

Faraz Sarmeili, Benyamin Ghahremani-Nezhad, Mohammad Khalilpour, Karim Abbasi, Rassoul Dinarvand, Hamid R. Rabiee

published 13 Aug 2026

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Assessing fish diversity in the Amazon: The impact of primers and reference libraries in eDNA surveys

Shizuka Hashimoto, Sarah V. Carvalho, Sonny de S. C. Miranda, Valéria N. Machado, Lucélia N. Carvalho, Cláudia P. de Deus, Izeni P. Farias, Tomas Hrbek

published 13 Aug 2026

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Optimization of the Mit1C real-time PCR assay using Bio-Rad CFX96 for the detection of Cyclospora cayetanensis in agricultural waters

Khai Truong, Miely Suarez, Mauricio Durigan, Laura Ewing-Peeples, Mark C. Jenkins, Aminata Kilungo, Margarethe A. Cooper, Kerry K. Cooper, Gerardo U. Lopez

published 12 Aug 2026

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Eosinophil-associated intestinal immune responses following SARS-CoV-2 infection in K18-hACE2 mice

Shun Tamaki, Rei Kawashima, Takayuki Uematsu, Fumitaka Kawakami, Motoki Imai, Yoshifumi Kurosaki, Toshihide Matsumoto, Shotaro Maehana, Yusuke Hara, Setsuko Sugawara, Hiroki Izawa, Takafumi Ichikawa, Hidero Kitasato, Hideaki Hanaki, Makoto Kubo

published 12 Aug 2026

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