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Molecular biology

A method for PCR-free library preparation for sequencing palaeogenomes

Kirstin Henneberger, Axel Barlow, [ ... ], Johanna L. A. Paijmans

DNA Barcode, chemical analysis, and antioxidant activity of Psidium guineense from Ecuador

Joel Eduardo Vielma-Puente, Efrén Santos-Ordóñez, [ ... ], Yuraima Fonseca

mRNA extracted from frozen buffy coat samples stored long term in tubes with no RNA preservative shows promise for downstream sequencing analyses

Erik Bovinder Ylitalo, Linda Vidman, Sophia Harlid, Bethany Van Guelpen

RDE & dynamometer analysis of light-duty vehicle emissions across altitudes, temperatures, and driving styles

Jiguang Wang, Li Wang, [ ... ], Xudong Chen

Single-cell RNA-seq reveals disease-specific CD8+ T cell clonal expansion and a high frequency of transcriptionally distinct double-negative T cells in diabetic NOD mice

Md Zohorul Islam, Sam Zimmerman, [ ... ], Michael Robben

In silico identification of novel ligands targeting stress-related human FKBP5 protein in mental disorders

Ovinuchi Ejiohuo, Donald Bajia, Joanna Pawlak, Aleksandra Szczepankiewicz

Digital PCR characterizes epithelial cell populations in murine duodenal organoids

Karla Acosta-Virgen, Hugo David González-Conchillos, [ ... ], Martha Espinosa-Cantellano

Diagnostic accuracy of multiplex real-time PCR for detecting viruses associated with encephalitis: A systematic review and meta-analysis protocol

Sharifah Aliah Diyanah Syed Hussin, Xin Wee Chen, [ ... ], Seok Mui Wang

Impact of spaceflight on gene expression in cultured human mesenchymal stem/stromal cells

Peng Huang, Bryan T. Piatkowski, [ ... ], Abba C. Zubair

Functional role for Taz during hindbrain ventricle morphogenesis

Renée Dicipulo, Lyndsay G. Selland, Rowan G. Carpenter, Andrew J. Waskiewicz

Revolutionizing Molecular cloning: Introducing FastCloneAssist, a Streamlined Python tool for optimizing primer design in restriction & ligation-independent PCR cloning

Pradip Kumar Singh, Michael S. Donnenberg

Deciphering the structural consequences of R83 and R152 methylation on DNA polymerase β using molecular modeling

Amit Srivastava, Haitham Idriss, [ ... ], Dirar Homouz

Comparative analysis of protein expression between oesophageal adenocarcinoma and normal adjacent tissue

Ben Nicholas, Alistair Bailey, [ ... ], Paul Skipp

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