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Macromolecular engineering

Bottom-up synthesis of protein-based nanomaterials from engineered β-solenoid proteins

Zeyu Peng, Maria D. R. Peralta, Daniel L. Cox, Michael D. Toney

Imaging the Directed Transport of Single Engineered RNA Transcripts in Real-Time Using Ratiometric Bimolecular Beacons

Xuemei Zhang, Allison L. Zajac, [ ... ], Andrew Tsourkas

Computational Design of the β-Sheet Surface of a Red Fluorescent Protein Allows Control of Protein Oligomerization

Timothy M. Wannier, Matthew M. Moore, Yun Mou, Stephen L. Mayo

Genetically Engineered Yeast Expressing a Lytic Peptide from Bee Venom (Melittin) Kills Symbiotic Protozoa in the Gut of Formosan Subterranean Termites

Claudia Husseneder, Jennifer R. Donaldson, Lane D. Foil

Probing the Effector and Suppressive Functions of Human T Cell Subsets Using Antigen-Specific Engineered T Cell Receptors

Qi Wan, Lina Kozhaya, [ ... ], Derya Unutmaz

Next generation calmodulin affinity purification: Clickable calmodulin facilitates improved protein purification

Julia G. Fraseur, Tamara L. Kinzer-Ursem

Directed Evolution of Aminoglycoside Phosphotransferase (3′) Type IIIa Variants That Inactivate Amikacin but Impose Significant Fitness Costs

Joseph R. Kramer, Ichiro Matsumura

Seven N-terminal Residues of a Thermophilic Xylanase Are Sufficient to Confer Hyperthermostability on Its Mesophilic Counterpart

Shan Zhang, Yongzhi He, Haiying Yu, Zhiyang Dong

Directed Evolution and Resolution Mechanism of 1, 3-Propanediol Oxidoreductase from Klebsiella pneumoniae toward Higher Activity by Error-Prone PCR and Bioinformatics

Wei Jiang, Yuan Zhuang, Shizhen Wang, Baishan Fang

The register shift rules for βαβ-motifs for de novo protein design

Hiroto Murata, Hayao Imakawa, Nobuyasu Koga, George Chikenji

5,5-Dialkylluciferins are thermal stable substrates for bioluminescence-based detection systems

Ce Shi, Michael P. Killoran, [ ... ], Thomas A. Kirkland

Computational design of novel nanobodies targeting the receptor binding domain of variants of concern of SARS-CoV-2

Phoomintara Longsompurana, Thanyada Rungrotmongkol, [ ... ], Rungtiva P. Poo-arporn

The scale-free nature of protein sequence space

Patrick C. F. Buchholz, Catharina Zeil, Jürgen Pleiss

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