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Macromolecular engineering

Protein Trans-Splicing of an Atypical Split Intein Showing Structural Flexibility and Cross-Reactivity

Huiling Song, Qing Meng, Xiang-Qin Liu

Complex Structure of Engineered Modular Domains Defining Molecular Interaction between ICAM-1 and Integrin LFA-1

Sungkwon Kang, Chae Un Kim, [ ... ], Moonsoo M. Jin

The PLOS ONE Synthetic Biology Collection: Six Years and Counting

Jean Peccoud, Mark Isalan

An Automated Flow for Directed Evolution Based on Detection of Promiscuous Scaffolds Using Spatial and Electrostatic Properties of Catalytic Residues

Sandeep Chakraborty

Structure of an Engineered β-Lactamase Maltose Binding Protein Fusion Protein: Insights into Heterotropic Allosteric Regulation

Wei Ke, Abigail H. Laurent, [ ... ], Focco van den Akker

Engineering of Three-Finger Fold Toxins Creates Ligands with Original Pharmacological Profiles for Muscarinic and Adrenergic Receptors

Carole Fruchart-Gaillard, Gilles Mourier, [ ... ], Denis Servent

Circular Permutation in the Ω-Loop of TEM-1 β-Lactamase Results in Improved Activity and Altered Substrate Specificity

Gurkan Guntas, Manu Kanwar, Marc Ostermeier

Incorporation of Noncanonical Amino Acids into Rosetta and Use in Computational Protein-Peptide Interface Design

P. Douglas Renfrew, Eun Jung Choi, Richard Bonneau, Brian Kuhlman

Engineered Protein Nano-Compartments for Targeted Enzyme Localization

Swati Choudhary, Maureen B. Quin, [ ... ], Claudia Schmidt-Dannert

Directed Evolution of the Transcriptional Regulator DntR: Isolation of Mutants with Improved DNT-Response

Rosa Lönneborg, Edina Varga, Peter Brzezinski

Stabilisation of the Fc Fragment of Human IgG1 by Engineered Intradomain Disulfide Bonds

Gordana Wozniak-Knopp, Johannes Stadlmann, Florian Rüker

A Thermostable β-Glucuronidase Obtained by Directed Evolution as a Reporter Gene in Transgenic Plants

Ai-Sheng Xiong, Ri-He Peng, [ ... ], Quan-Hong Yao

The Molecular Basis for the Broad Substrate Specificity of Human Sulfotransferase 1A1

Ilana Berger, Chen Guttman, [ ... ], Amir Aharoni

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