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Macromolecular engineering

Desired Alteration of Protein Affinities: Competitive Selection of Protein Variants Using Yeast Signal Transduction Machinery

Misato Kaishima, Nobuo Fukuda, Jun Ishii, Akihiko Kondo

On Simplified Global Nonlinear Function for Fitness Landscape: A Case Study of Inverse Protein Folding

Yun Xu, Changyu Hu, Yang Dai, Jie Liang

Improving the Anti-Toxin Abilities of the CMG2-Fc Fusion Protein with the Aid of Computational Design

Yongyi Xi, Xiaojie Wu, [ ... ], Junjie Yue

Computational Design of Binding Proteins to EGFR Domain II

Yoon Sup Choi, Soomin Yoon, [ ... ], Sung Ho Ryu

A High-Throughput Colorimetric Screening Assay for Terpene Synthase Activity Based on Substrate Consumption

Maiko Furubayashi, Mayu Ikezumi, [ ... ], Daisuke Umeno

Mutagenic Organized Recombination Process by Homologous In Vivo Grouping (MORPHING) for Directed Enzyme Evolution

David Gonzalez-Perez, Patricia Molina-Espeja, Eva Garcia-Ruiz, Miguel Alcalde

Seven N-terminal Residues of a Thermophilic Xylanase Are Sufficient to Confer Hyperthermostability on Its Mesophilic Counterpart

Shan Zhang, Yongzhi He, Haiying Yu, Zhiyang Dong

Improving Kinetic or Thermodynamic Stability of an Azoreductase by Directed Evolution

Vânia Brissos, Nádia Gonçalves, Eduardo P. Melo, Lígia O. Martins

Tracking Molecular Recognition at the Atomic Level with a New Protein Scaffold Based on the OB-Fold

John D. Steemson, Matthias Baake, [ ... ], Mark T. Liddament

Imaging the Directed Transport of Single Engineered RNA Transcripts in Real-Time Using Ratiometric Bimolecular Beacons

Xuemei Zhang, Allison L. Zajac, [ ... ], Andrew Tsourkas

Improving Glyphosate Oxidation Activity of Glycine Oxidase from Bacillus cereus by Directed Evolution

Tao Zhan, Kai Zhang, [ ... ], Ziduo Liu

Directed Evolution of Aminoglycoside Phosphotransferase (3′) Type IIIa Variants That Inactivate Amikacin but Impose Significant Fitness Costs

Joseph R. Kramer, Ichiro Matsumura

Mechanism of Protein Kinetic Stabilization by Engineered Disulfide Crosslinks

Inmaculada Sanchez-Romero, Antonio Ariza, [ ... ], Jose M. Sanchez-Ruiz

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