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Macromolecular engineering

On Simplified Global Nonlinear Function for Fitness Landscape: A Case Study of Inverse Protein Folding

Yun Xu, Changyu Hu, Yang Dai, Jie Liang

published 11 Aug 2014

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Improving the Anti-Toxin Abilities of the CMG2-Fc Fusion Protein with the Aid of Computational Design

Yongyi Xi, Xiaojie Wu, Lihua Gao, Yong Shao, Hui Peng, Hongxing Chen, Huipeng Chen, Xianwen Hu, Junjie Yue

published 07 Aug 2014

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Computational Design of Binding Proteins to EGFR Domain II

Yoon Sup Choi, Soomin Yoon, Kyung-Lock Kim, Jiho Yoo, Parkyong Song, Minsoo Kim, Young-Eun Shin, Won Jun Yang, Jung-eun Noh, Hyun-soo Cho, Sanguk Kim, Junho Chung, Sung Ho Ryu

published 07 Apr 2014

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A High-Throughput Colorimetric Screening Assay for Terpene Synthase Activity Based on Substrate Consumption

Maiko Furubayashi, Mayu Ikezumi, Jun Kajiwara, Miki Iwasaki, Akira Fujii, Ling Li, Kyoichi Saito, Daisuke Umeno

published 28 Mar 2014

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Mutagenic Organized Recombination Process by Homologous In Vivo Grouping (MORPHING) for Directed Enzyme Evolution

David Gonzalez-Perez, Patricia Molina-Espeja, Eva Garcia-Ruiz, Miguel Alcalde

published 10 Mar 2014

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Seven N-terminal Residues of a Thermophilic Xylanase Are Sufficient to Confer Hyperthermostability on Its Mesophilic Counterpart

Shan Zhang, Yongzhi He, Haiying Yu, Zhiyang Dong

published 30 Jan 2014

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Improving Kinetic or Thermodynamic Stability of an Azoreductase by Directed Evolution

Vânia Brissos, Nádia Gonçalves, Eduardo P. Melo, Lígia O. Martins

published 27 Jan 2014

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Tracking Molecular Recognition at the Atomic Level with a New Protein Scaffold Based on the OB-Fold

John D. Steemson, Matthias Baake, Jasna Rakonjac, Vickery L. Arcus, Mark T. Liddament

published 20 Jan 2014

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Imaging the Directed Transport of Single Engineered RNA Transcripts in Real-Time Using Ratiometric Bimolecular Beacons

Xuemei Zhang, Allison L. Zajac, Lingyan Huang, Mark A. Behlke, Andrew Tsourkas

published 15 Jan 2014

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Improving Glyphosate Oxidation Activity of Glycine Oxidase from Bacillus cereus by Directed Evolution

Tao Zhan, Kai Zhang, Yangyan Chen, Yongjun Lin, Gaobing Wu, Lili Zhang, Pei Yao, Zongze Shao, Ziduo Liu

published 05 Nov 2013

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Directed Evolution of Aminoglycoside Phosphotransferase (3′) Type IIIa Variants That Inactivate Amikacin but Impose Significant Fitness Costs

Joseph R. Kramer, Ichiro Matsumura

published 18 Oct 2013

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Mechanism of Protein Kinetic Stabilization by Engineered Disulfide Crosslinks

Inmaculada Sanchez-Romero, Antonio Ariza, Keith S. Wilson, Michael Skjøt, Jesper Vind, Leonardo De Maria, Lars K. Skov, Jose M. Sanchez-Ruiz

published 30 Jul 2013

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Fitness Loss and Library Size Determination in Saturation Mutagenesis

Yuval Nov

published 03 Jul 2013

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