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Genome engineering

A Rac1-FMNL2 signaling module affects cell-cell contact formation independent of Cdc42 and membrane protrusions

Hanna Grobe, Andrea Wüstenhagen, [ ... ], Katharina Grikscheit

Rats deficient C-type natriuretic peptide suffer from impaired skeletal growth without early death

Toshihito Fujii, Keisho Hirota, [ ... ], Kazuwa Nakao

Characterization of knockin mice at the Rosa26, Tac1 and Plekhg1 loci generated by homologous recombination in oocytes

Youmei Wu, María José Luna, [ ... ], James M. Pickel

A dystrophic Duchenne mouse model for testing human antisense oligonucleotides

Marcel Veltrop, Laura van Vliet, [ ... ], Maaike van Putten

Cas9-mediated excision of proximal DNaseI/H3K4me3 signatures confers robust silencing of microRNA and long non-coding RNA genes

Harshavardhan Janga, Marina Aznaourova, [ ... ], Leon N. Schulte

Lactobacillus gasseri CRISPR-Cas9 characterization In Vitro reveals a flexible mode of protospacer-adjacent motif recognition

Emily M. Anderson, Shawn McClelland, [ ... ], Anja van Brabant Smith

Tailor-made gene silencing of Staphylococcus aureus clinical isolates by CRISPR interference

Yusuke Sato’o, Junzo Hisatsune, [ ... ], Motoyuki Sugai

The endocannabinoid gene faah2a modulates stress-associated behavior in zebrafish

Randall G. Krug II, Han B. Lee, [ ... ], Karl J. Clark

Genomic features of “Candidatus Venteria ishoeyi”, a new sulfur-oxidizing macrobacterium from the Humboldt Sulfuretum off Chile

Alexis Fonseca, Thomas Ishoey, [ ... ], Víctor A. Gallardo

Versatile single-step-assembly CRISPR/Cas9 vectors for dual gRNA expression

Fatwa Adikusuma, Chandran Pfitzner, Paul Quinton Thomas

Minimal 2'-O-methyl phosphorothioate linkage modification pattern of synthetic guide RNAs for increased stability and efficient CRISPR-Cas9 gene editing avoiding cellular toxicity

Megan Basila, Melissa L. Kelley, Anja van Brabant Smith

indCAPS: A tool for designing screening primers for CRISPR/Cas9 mutagenesis events

Charles Hodgens, Zachary L. Nimchuk, Joseph J. Kieber

Establishment of pten knockout medaka with transcription activator–like effector nucleases (TALENs) as a model of PTEN deficiency disease

Yuriko Matsuzaki, Tetsushi Sakuma, Takashi Yamamoto, Hideyuki Saya

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