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Genome engineering

Minimal 2'-O-methyl phosphorothioate linkage modification pattern of synthetic guide RNAs for increased stability and efficient CRISPR-Cas9 gene editing avoiding cellular toxicity

Megan Basila, Melissa L. Kelley, Anja van Brabant Smith

indCAPS: A tool for designing screening primers for CRISPR/Cas9 mutagenesis events

Charles Hodgens, Zachary L. Nimchuk, Joseph J. Kieber

Establishment of pten knockout medaka with transcription activator–like effector nucleases (TALENs) as a model of PTEN deficiency disease

Yuriko Matsuzaki, Tetsushi Sakuma, Takashi Yamamoto, Hideyuki Saya

CRISPR/Cas-mediated knock-in via non-homologous end-joining in the crustacean Daphnia magna

Hitoshi Kumagai, Takashi Nakanishi, [ ... ], Hajime Watanabe

Diversity and microevolution of CRISPR loci in Helicobacter cinaedi

Junko Tomida, Yuji Morita, [ ... ], Yoshiaki Kawamura

Generation of beta-lactoglobulin knock-out goats using CRISPR/Cas9

Wenjun Zhou, Yongjie Wan, [ ... ], Feng Wang

Efficient generation of mutations mediated by CRISPR/Cas9 in the hairy root transformation system of Brassica carinata

Thomas W. Kirchner, Markus Niehaus, [ ... ], Marco Herde

Restriction-modification mediated barriers to exogenous DNA uptake and incorporation employed by Prevotella intermedia

Christopher D. Johnston, Chelsey A. Skeete, [ ... ], Susan R. Rittling

Functional characterization of zebrafish orthologs of the human Beta 3-Glucosyltransferase B3GLCT gene mutated in Peters Plus Syndrome

Eric Weh, Hideyuki Takeuchi, [ ... ], Elena V. Semina

CRISPR-FOCUS: A web server for designing focused CRISPR screening experiments

Qingyi Cao, Jian Ma, [ ... ], X. Shirley Liu

Genetic dissection of endothelial transcriptional activity of zebrafish aryl hydrocarbon receptors (AHRs)

Wade W. Sugden, Roberto C. Leonardo-Mendonça, Darío Acuña-Castroviejo, Arndt F. Siekmann

Highly efficient gene inactivation by adenoviral CRISPR/Cas9 in human primary cells

Olaf Voets, Frans Tielen, [ ... ], Christian Essrich

Comparative pathogenomics of Clostridium tetani

Jonathan E. Cohen, Rong Wang, [ ... ], James E. Keller

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