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Genetic engineering

A novel soybean hairy root system for gene functional validation

Bruna Medeiros Pereira, Fabrício Arraes, Andressa Cunha Quintana Martins, Nayara Sabrina Freitas Alves, Bruno Paes Melo, Carolina Vianna Morgante, Mario Alfredo Passos Saraiva, Maria Fátima Grossi-de-Sá, Patricia Messenberg Guimaraes, Ana Cristina Miranda Brasileiro

published 18 May 2023

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Genetically modified crops do not present variations in pollen viability and morphology when compared to their conventional counterparts

Daniel Soares, Hallison Vertuan, Fabiana Bacalhau, Marcia José, Augusto Crivellari, Gustavo G. Belchior, Geraldo U. Berger

published 01 May 2023

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A comparison of machine learning approaches for the quantification of microglial cells in the brain of mice, rats and non-human primates

Danish M. Anwer, Francesco Gubinelli, Yunus A. Kurt, Livija Sarauskyte, Febe Jacobs, Chiara Venuti, Ivette M. Sandoval, Yiyi Yang, Jennifer Stancati, Martina Mazzocchi, Edoardo Brandi, Gerard O’Keeffe, Kathy Steece-Collier, Jia-Yi Li, Tomas Deierborg, Fredric P. Manfredsson, Marcus Davidsson, Andreas Heuer

published 01 May 2023

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Synonymous alterations of cancer-associated Trp53 CpG mutational hotspots cause fatal developmental jaw malocclusions but no tumors in knock-in mice

Richard J. Epstein, Frank P. Y. Lin, Robert A. Brink, James Blackburn

published 13 Apr 2023

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Bioengineering of a Lactococcus lactis subsp. lactis strain enhances nisin production and bioactivity

Roxana Portieles, Hongli Xu, Feng Chen, Jingyao Gao, Lihua Du, Xiangyou Gao, Carlos Borroto Nordelo, Qiulin Yue, Lin Zhao, Nayanci Portal Gonzalez, Ramon Santos Bermudez, Orlando Borrás-Hidalgo

published 10 Apr 2023

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Preclinical trials in Alzheimer’s disease: Sample size and effect size for behavioural and neuropathological outcomes in 5xFAD mice

Mahvish Faisal, Jana Aid, Bekzod Nodirov, Benjamin Lee, Miriam A. Hickey

published 10 Apr 2023

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In vitro spermatogenesis in isolated seminiferous tubules of immature mice

Xuemin Feng, Takafumi Matsumura, Yuki Yamashita, Takuya Sato, Kiyoshi Hashimoto, Hisakazu Odaka, Yoshinori Makino, Yuki Okada, Hiroko Nakamura, Hiroshi Kimura, Teruo Fujii, Takehiko Ogawa

published 06 Apr 2023

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A seeding-based neuronal model of tau aggregation for use in drug discovery

Ines S. Amorim, Sylvie Challal, Laetitia Cistarelli, Thierry Dorval, Laurene Abjean, Manuelle Touzard, Nicolas Arbez, Arnaud François, Fany Panayi, Ross Jeggo, Erika Cecon, Atsuro Oishi, Julie Dam, Ralf Jockers, Patricia Machado

published 04 Apr 2023

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High signal-to-noise imaging of spontaneous and 5 ns electric pulse-evoked Ca²⁺ signals in GCaMP6f-expressing adrenal chromaffin cells isolated from transgenic mice

Ciara Viola, Thomas W. Gould, Nicole Procacci, Normand Leblanc, Josette Zaklit, Gale L. Craviso

published 31 Mar 2023

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Precision engineering of biological function with large-scale measurements and machine learning

Drew S. Tack, Peter D. Tonner, Abe Pressman, Nathan D. Olson, Sasha F. Levy, Eugenia F. Romantseva, Nina Alperovich, Olga Vasilyeva, David Ross

published 29 Mar 2023

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Mitotic CDK1 and 4E-BP1 II: A single phosphomimetic mutation in 4E-BP1 induces glucose intolerance in mice

Simon Cao, Michael J. Jurczak, Yoko Shuda, Rui Sun, Masahiro Shuda, Yuan Chang, Patrick S. Moore

published 10 Mar 2023

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Targeting nonsense-mediated RNA decay does not increase progranulin levels in the Grn ^R493X mouse model of frontotemporal dementia

Denise M. Smith, Michael L. Niehoff, Karen Ling, Paymaan Jafar-Nejad, Frank Rigo, Susan A. Farr, Miles F. Wilkinson, Andrew D. Nguyen

published 09 Mar 2023

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An optimized electrotransformation protocol for Lactobacillus jensenii

Elsa Fristot, Thomas Bessede, Miguel Camacho Rufino, Pauline Mayonove, Hung-Ju Chang, Ana Zuniga, Anne-Laure Michon, Sylvain Godreuil, Jérôme Bonnet, Guillaume Cambray

published 17 Feb 2023

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