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Gene cloning

pET28g: A Golden Gate-compatible pET vector for protein expression in Escherichia coli, validated by production of functional human ACE2

Inês M. Luís, Mariana Parada, João B. Vicente, Isabel A. Abreu

published 07 Jul 2025

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Susceptibility profile of Anopheles and target site resistance mechanism against organophosphates in Cameroon.

Judith Dandi-Labou, Jonas A. Kengne-Ouafo, Leon Mugenzi, Magellan Tchouakui, Murielle Wondji, Charles S. Wondji

published 22 May 2025

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Transcriptomic analysis of benznidazole-resistant Trypanosoma cruzi clone reveals nitroreductase I-independent resistance mechanisms

Ana María Mejía-Jaramillo, Hader Ospina-Zapata, Geysson Javier Fernandez, Omar Triana-Chávez

published 18 Feb 2025

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Evidence for gene essentiality in Leishmania using CRISPR

Wen-Wei Zhang, Greg Matlashewski

published 30 Dec 2024

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A cell competition system with one gene expression from a single-copy gene in one cell

Yoshinori Hasegawa, Megumi Nakano, Tsutomu Hosouchi, Takashi Watanabe, Izumi Yamaguchi, Manabu Nakayama, Osamu Ohara

published 05 Jul 2024

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Unraveling the impact of AXIN1 mutations on HCC development: Insights from CRISPR/Cas9 repaired AXIN1-mutant liver cancer cell lines

Ruyi Zhang, Shanshan Li, Kelly Schippers, Boaz Eimers, Jiahui Niu, Bastian V. H. Hornung, Mirjam C. G. N. van den Hout, Wilfred F. J. van Ijcken, Maikel P. Peppelenbosch, Ron Smits

published 07 Jun 2024

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HMGR and CHS gene cloning, characterizations and tissue-specific expressions in Polygala tenuifolia Willd

Yang Liu, Xiaofang Ma, Fuying Mao, Jinmiao Qiu, Jingyi Bi, Xiaowei Li, Xian Gu, Yuguang Zheng, Yunsheng Zhao

published 25 Mar 2024

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Generation of murine tumor models refractory to αPD-1/-L1 therapies due to defects in antigen processing/presentation or IFNγ signaling using CRISPR/Cas9

Paul L. Chariou, Christine M. Minnar, Mayank Tandon, Mary R. Guest, Raj Chari, Jeffrey Schlom, Sofia R. Gameiro

published 01 Mar 2024

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Resistome and virulome of high-risk pandemic clones of multidrug-resistant extra-intestinal pathogenic Escherichia coli (ExPEC) isolated from tertiary healthcare settings in Uganda

Denis K. Byarugaba, Bernard Erima, Godfrey Wokorach, Stephen Alafi, Hannah Kibuuka, Edison Mworozi, Ambrose K. Musinguzi, James Kiyengo, Florence Najjuka, Fred Wabwire-Mangen

published 22 Nov 2023

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Functional characterization of a single nucleotide polymorphism associated with Alzheimer’s disease in a hiPSC-based neuron model

Lindsay R. Stolzenburg, Sahar Esmaeeli, Ameya S. Kulkarni, Erin Murphy, Taekyung Kwon, Christina Preiss, Lamiaa Bahnassawy, Joshua D. Stender, Justine D. Manos, Peter Reinhardt, Fedik Rahimov, Jeffrey F. Waring, Cyril Y. Ramathal

published 26 Sep 2023

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Bioengineering of a Lactococcus lactis subsp. lactis strain enhances nisin production and bioactivity

Roxana Portieles, Hongli Xu, Feng Chen, Jingyao Gao, Lihua Du, Xiangyou Gao, Carlos Borroto Nordelo, Qiulin Yue, Lin Zhao, Nayanci Portal Gonzalez, Ramon Santos Bermudez, Orlando Borrás-Hidalgo

published 10 Apr 2023

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In vitro assembly of plasmid DNA for direct cloning in Lactiplantibacillus plantarum WCSF1

Marc Blanch-Asensio, Sourik Dey, Shrikrishnan Sankaran

published 16 Feb 2023

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High-throughput FastCloning technology: A low-cost method for parallel cloning

Hua Jiang, Fan Meng, Deren Lu, Yanjuan Chen, Guilin Luo, Yuejun Chen, Jun Chen, Cheng Chen, Xi Zhang, Dan Su

published 09 Sep 2022

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