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Electrophoretic techniques

Efficient DNA extraction from cytogenetic suspensions: A new possibility for obtaining DNA, with potential applications in studies of molecular markers

Geórgia Liz Monteiro Sant’ana-Arruda, Paulo Cesar Venere, Daniela Cristina Ferreira

published 07 Nov 2025

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Validation of blue- and clear-native polyacrylamide gel electrophoresis protocols to characterize mitochondrial oxidative phosphorylation complexes

Jana Aref, Seungtae Lee, Supachaya Sriphoosanaphan, Micol Falabella, Shi-Yu Yang, Jan-Willem Taanman

published 18 Sep 2025

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Dissecting the dynamics of virus-derived DNA of dengue virus 2 (DENV-2) in Aedes mosquitoes

Hiroka Aonuma, Aboubacar Sombié, Jian-Chiuan Li, Manabu Ote, Erisha Saiki, Hidetoshi Ichimura, Itoe Iizuka, Taichi Odagawa, Tatsuya Sakurai, Kayoko Yamaji, Masayuki Saijo, Chun-Hong Chen, Athanase Badolo, Hirotaka Kanuka

published 12 Sep 2025

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Association of FGF Receptor-2rs2981578 SNP with breast cancer in women at the University of Gondar Comprehensive Specialized Hospital, Ethiopia

Mihiret Bogale, Tiget Ayelgn Mengstie, Ephrem Tadesse, Tadesse Asmamaw Dejenie, Banchamlak Teferi, Melkamu Siferih, Mulualem Nibret Takle, Tseganesh Asefa, Gashaw Dessie, Winta Tesfaye, Endeshaw Asaye Kindie, Hiwot Tezera Endale, Kibur Hunie Tesfa, Birhanu Ayelign, Tewodros Shibabaw

published 01 Aug 2025

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Superior normalization using total protein for western blot analysis of human adipocytes

Leo J.S. Westerberg, Benjamin Dedic, Erik Näslund, Anders Thorell, Kirsty L. Spalding

published 22 Jul 2025

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Conversion of a telomere resolvase into a Cre-like site-specific recombinase

Shu Hui Huang, Mahrokh Balouchi, Kerri Kobryn

published 17 Jul 2025

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Development and validation of versatile species-specific primer assays for eDNA monitoring and authentication of 10 commercially important Peruvian marine species

Alan Marín, Ruben Alfaro, Lorenzo E. Reyes-Flores, Claudia Ingar, Luis E. Santos-Rojas, Irina B. Alvarez-Jaque, Karen Rodríguez-Bernales, Cleila Carbajal, Angel Yon-Utrilla, Eliana Zelada-Mázmela

published 02 Jul 2025

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Differential interactions of ToLCNDV with different betasatellites reveal complex viral dynamics in N. benthamiana

Zafar Iqbal, Muhammad Shafiq, Sajed Ali, Mudassar Fareed Awan, Muhammad Farhan Sarwar, Imran Amin, Muhammad Shafiq Shahid, Rob W. Briddon

published 25 Jun 2025

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Physicochemical quality assessment of four asparaginases

Vanessa Radtke, Thorsten König, Christoph Radcke, Ulf Bergmann, Rene Eichler, Katja J. Pohl, Arndt Schnuchel

published 16 Jun 2025

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Titration-WB: A methodology for accurate quantitative protein determination overcoming reproducibility errors

Alice Maestri, Ewa Ehrenborg, Olivera Werngren, Maria Olin, Carolina E. Hagberg, Matteo Pedrelli, Paolo Parini

published 12 Jun 2025

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Perish the thawed? EDTA reduces DNA degradation during extraction from frozen tissue

Ella Messner, Lev Becker, Mia L. DeSanctis, Elizabeth A. Soranno, Ryan Pianka, Caileigh Pierce, Molly Johnson, Rosalia Falco Poulin, Hannah J. Appiah-Madson, Daniel L. Distel

published 03 Jun 2025

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T7 RNA polymerase-based gene expression from a transcriptionally silent rDNA spacer in the endosymbiont-harboring trypanosomatid Angomonas deanei

Lena Kröninger, Anay K. Maurya, Christian Stiebeling, Florian P. Stirba, Zio Kim, Eva C. M. Nowack

published 30 May 2025

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Making the best of a bad sample: Comparison of DNA extraction and quantification methods using sub-optimally stored Ixodes ricinus ticks

Andrea P. Cotes-Perdomo, Kenedith Méndez-Gutierrez, Kristian Alfsnes, Åshild K. Andreassen, Andrew Jenkins

published 29 May 2025

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