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DNA libraries

A novel CRISPR/Cas9 associated technology for sequence-specific nucleic acid enrichment

Richard C. Stevens, Jennifer L. Steele, William R. Glover, Jorge F. Sanchez-Garcia, Stephen D. Simpson, Devon O’Rourke, Jordan S. Ramsdell, Matthew D. MacManes, W. Kelley Thomas, Anthony P. Shuber

published 18 Apr 2019

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Use of FFPE-derived DNA in next generation sequencing: DNA extraction methods

Samantha J. McDonough, Aditya Bhagwate, Zhifu Sun, Chen Wang, Michael Zschunke, Joshua A. Gorman, Karla J. Kopp, Julie M. Cunningham

published 11 Apr 2019

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Fragmentation Through Polymerization (FTP): A new method to fragment DNA for next-generation sequencing

Konstantin B. Ignatov, Konstantin A. Blagodatskikh, Dmitry S. Shcherbo, Tatiana V. Kramarova, Yulia A. Monakhova, Vladimir M. Kramarov

published 01 Apr 2019

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Integrated routine workflow using next-generation sequencing and a fully-automated platform for the detection of KRAS, NRAS and BRAF mutations in formalin-fixed paraffin embedded samples with poor DNA quality in patients with colorectal carcinoma

Claire Franczak, Ludovic Dubouis, Pauline Gilson, Marie Husson, Marie Rouyer, Jessica Demange, Agnès Leroux, Jean-Louis Merlin, Alexandre Harlé

published 27 Feb 2019

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Arbuscular mycorrhizal fungi induce the expression of specific retrotransposons in roots of sunflower (Helianthus annuus L.)

Alberto Vangelisti, Flavia Mascagni, Tommaso Giordani, Cristiana Sbrana, Alessandra Turrini, Andrea Cavallini, Manuela Giovannetti, Lucia Natali

published 19 Feb 2019

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Testing of library preparation methods for transcriptome sequencing of real life glioblastoma and brain tissue specimens: A comparative study with special focus on long non-coding RNAs

Marek Vecera, Jiri Sana, Jan Oppelt, Boris Tichy, Kopkova Alena, Radim Lipina, Martin Smrcka, Radim Jancalek, Marketa Hermanova, Leos Kren, Ondrej Slaby

published 11 Feb 2019

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Low-cost cross-taxon enrichment of mitochondrial DNA using in-house synthesised RNA probes

Stephen M. Richards, Nelli Hovhannisyan, Matthew Gilliham, Joshua Ingram, Birgitte Skadhauge, Holly Heiniger, Bastien Llamas, Kieren J. Mitchell, Julie Meachen, Geoffrey B. Fincher, Jeremy J. Austin, Alan Cooper

published 04 Feb 2019

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Miniaturization and optimization of 384-well compatible RNA sequencing library preparation

Madeline Y. Mayday, Lillian M. Khan, Eric D. Chow, Matt S. Zinter, Joseph L. DeRisi

published 10 Jan 2019

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A user-friendly platform for yeast two-hybrid library screening using next generation sequencing

Marie-Laure Erffelinck, Bianca Ribeiro, Maria Perassolo, Laurens Pauwels, Jacob Pollier, Veronique Storme, Alain Goossens

published 21 Dec 2018

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Rapid and highly-specific generation of targeted DNA sequencing libraries enabled by linking capture probes with universal primers

Joel Pel, Amy Leung, Wendy W. Y. Choi, Milenko Despotovic, W. Lloyd Ung, Gosuke Shibahara, Laura Gelinas, Andre Marziali

published 05 Dec 2018

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Ribosome display for the rapid generation of high-affinity Zika-neutralizing single-chain antibodies

Adinarayana Kunamneni, Chunyan Ye, Steven B. Bradfute, Ravi Durvasula

published 16 Nov 2018

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Comparison of normalization approaches for gene expression studies completed with high-throughput sequencing

Farnoosh Abbas-Aghababazadeh, Qian Li, Brooke L. Fridley

published 31 Oct 2018

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Investigation of the viral and bacterial microbiota in intestinal samples from mink (Neovison vison) with pre-weaning diarrhea syndrome using next generation sequencing

Julie Melsted Birch, Karin Ullman, Tina Struve, Jens Frederik Agger, Anne Sofie Hammer, Mikael Leijon, Henrik Elvang Jensen

published 18 Oct 2018

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