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DNA libraries

Reading canonical and modified nucleobases in 16S ribosomal RNA using nanopore native RNA sequencing

Andrew M. Smith, Miten Jain, Logan Mulroney, Daniel R. Garalde, Mark Akeson

published 16 May 2019

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Immunocapture of virions with virus-specific antibodies prior to high-throughput sequencing effectively enriches for virus-specific sequences

Dennis Knierim, Wulf Menzel, Stephan Winter

published 09 May 2019

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Robustness of RNA sequencing on older formalin-fixed paraffin-embedded tissue from high-grade ovarian serous adenocarcinomas

Yongmei Zhao, Monika Mehta, Ashley Walton, Keyur Talsania, Yelena Levin, Jyoti Shetty, Elizabeth M. Gillanders, Bao Tran, Danielle Mercatante Carrick

published 06 May 2019

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Reference genes selection for Calotropis procera under different salt stress conditions

Maria R. V. Coêlho, Rebeca Rivas, José Ribamar C. Ferreira-Neto, Valesca Pandolfi, João P. Bezerra-Neto, Ana Maria Benko-Iseppon, Mauro G. Santos

published 18 Apr 2019

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A novel CRISPR/Cas9 associated technology for sequence-specific nucleic acid enrichment

Richard C. Stevens, Jennifer L. Steele, William R. Glover, Jorge F. Sanchez-Garcia, Stephen D. Simpson, Devon O’Rourke, Jordan S. Ramsdell, Matthew D. MacManes, W. Kelley Thomas, Anthony P. Shuber

published 18 Apr 2019

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Use of FFPE-derived DNA in next generation sequencing: DNA extraction methods

Samantha J. McDonough, Aditya Bhagwate, Zhifu Sun, Chen Wang, Michael Zschunke, Joshua A. Gorman, Karla J. Kopp, Julie M. Cunningham

published 11 Apr 2019

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Fragmentation Through Polymerization (FTP): A new method to fragment DNA for next-generation sequencing

Konstantin B. Ignatov, Konstantin A. Blagodatskikh, Dmitry S. Shcherbo, Tatiana V. Kramarova, Yulia A. Monakhova, Vladimir M. Kramarov

published 01 Apr 2019

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Integrated routine workflow using next-generation sequencing and a fully-automated platform for the detection of KRAS, NRAS and BRAF mutations in formalin-fixed paraffin embedded samples with poor DNA quality in patients with colorectal carcinoma

Claire Franczak, Ludovic Dubouis, Pauline Gilson, Marie Husson, Marie Rouyer, Jessica Demange, Agnès Leroux, Jean-Louis Merlin, Alexandre Harlé

published 27 Feb 2019

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Arbuscular mycorrhizal fungi induce the expression of specific retrotransposons in roots of sunflower (Helianthus annuus L.)

Alberto Vangelisti, Flavia Mascagni, Tommaso Giordani, Cristiana Sbrana, Alessandra Turrini, Andrea Cavallini, Manuela Giovannetti, Lucia Natali

published 19 Feb 2019

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Testing of library preparation methods for transcriptome sequencing of real life glioblastoma and brain tissue specimens: A comparative study with special focus on long non-coding RNAs

Marek Vecera, Jiri Sana, Jan Oppelt, Boris Tichy, Kopkova Alena, Radim Lipina, Martin Smrcka, Radim Jancalek, Marketa Hermanova, Leos Kren, Ondrej Slaby

published 11 Feb 2019

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Low-cost cross-taxon enrichment of mitochondrial DNA using in-house synthesised RNA probes

Stephen M. Richards, Nelli Hovhannisyan, Matthew Gilliham, Joshua Ingram, Birgitte Skadhauge, Holly Heiniger, Bastien Llamas, Kieren J. Mitchell, Julie Meachen, Geoffrey B. Fincher, Jeremy J. Austin, Alan Cooper

published 04 Feb 2019

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Miniaturization and optimization of 384-well compatible RNA sequencing library preparation

Madeline Y. Mayday, Lillian M. Khan, Eric D. Chow, Matt S. Zinter, Joseph L. DeRisi

published 10 Jan 2019

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A user-friendly platform for yeast two-hybrid library screening using next generation sequencing

Marie-Laure Erffelinck, Bianca Ribeiro, Maria Perassolo, Laurens Pauwels, Jacob Pollier, Veronique Storme, Alain Goossens

published 21 Dec 2018

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