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cDNA libraries

A gene-specific RNA enrichment protocol for nanopore direct-RNA sequencing

Maja Bele Dyrendalsli, Cecilie Løkke, Christer Einvik

Evaluation of metatranscriptomic sequencing protocols to obtain full-length RNA virus genomes from mammalian tissues

Yiqiao Li, Mariana Polychronopoulou, [ ... ], Magda Bletsa

Reliable RNA-seq analysis from FFPE specimens as a means to accelerate cancer-related health disparities research

Mitchell J. Frederick, Dannelys Perez-Bello, [ ... ], Vlad C. Sandulache

Next-generation sequencing protocol of hematopoietic stem cells (HSCs). Step-by-step overview and troubleshooting guide

Justyna Jarczak, Kamila Bujko, [ ... ], Magdalena Kucia

Spatial transcriptomic profiling of isolated microregions in tissue sections utilizing laser-induced forward transfer

Kaiqiang Ye, Wanqing Chang, [ ... ], Xiangwei Zhao

A cell competition system with one gene expression from a single-copy gene in one cell

Yoshinori Hasegawa, Megumi Nakano, [ ... ], Osamu Ohara

RNA-seq RNAaccess identified as the preferred method for gene expression analysis of low quality FFPE samples

Kai Song, Emon Elboudwarej, [ ... ], Marianna Zavodovskaya

A versatile nuclei extraction protocol for single nucleus sequencing in non-model species–Optimization in various Atlantic salmon tissues

Rose Ruiz Daniels, Richard S. Taylor, [ ... ], Diego Robledo

High throughput isolation of RNA from single-cells within an intact tissue for spatial and temporal sequencing a reality

John Stanley, Akshar Lohith, [ ... ], Nader Pourmand

Pervasive mRNA uridylation in fission yeast is catalysed by both Cid1 and Cid16 terminal uridyltransferases

Lidia Lipińska-Zubrycka, Maciej Grochowski, Jürg Bähler, Michał Małecki

Optimised protocol for monitoring SARS-CoV-2 in wastewater using reverse complement PCR-based whole-genome sequencing

Harry T. Child, Paul A. O’Neill, [ ... ], Aaron R. Jeffries

Bioinformatic analysis predicts that ethanol exposure during early development causes alternative splicing alterations of genes involved in RNA post-transcriptional regulation

Camilo Fuentes-Beals, Montserrat Olivares-Costa, [ ... ], Fernando Faunes

A novel eukaryotic RdRP-dependent small RNA pathway represses antiviral immunity by controlling an ERK pathway component in the black-legged tick

Canran Feng, Kyosuke Torimaru, [ ... ], Katsutomo Okamura

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