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DNA libraries

Adaptation of CUT&RUN for use in African trypanosomes

Geneva Miller, Lindsey M. Rollosson, Carrie Saada, Serenity J. Wade, Danae Schulz

published 21 Nov 2023

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RNA-seq RNAaccess identified as the preferred method for gene expression analysis of low quality FFPE samples

Kai Song, Emon Elboudwarej, Xi Zhao, Luting Zhuo, David Pan, Jinfeng Liu, Carrie Brachmann, Scott D. Patterson, Oh Kyu Yoon, Marianna Zavodovskaya

published 26 Oct 2023

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A versatile nuclei extraction protocol for single nucleus sequencing in non-model species–Optimization in various Atlantic salmon tissues

Rose Ruiz Daniels, Richard S. Taylor, Ross Dobie, Sarah Salisbury, James J. Furniss, Emily Clark, Daniel J. Macqueen, Diego Robledo

published 07 Sep 2023

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High throughput isolation of RNA from single-cells within an intact tissue for spatial and temporal sequencing a reality

John Stanley, Akshar Lohith, Lucca Debiaso, Kevan Wang, Minh Ton, Wenwu Cui, Weiwei Gu, Aihua Fu, Nader Pourmand

published 01 Aug 2023

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Expression analysis of box C/D snoRNAs with SNPs between C57BL/6 and MSM/Ms strains in male mouse

Rumiko Saito, Maina Otsu, Hidenori Kiyosawa, Gota Kawai

published 10 Jul 2023

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Pervasive mRNA uridylation in fission yeast is catalysed by both Cid1 and Cid16 terminal uridyltransferases

Lidia Lipińska-Zubrycka, Maciej Grochowski, Jürg Bähler, Michał Małecki

published 23 May 2023

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Introducing an rbcL and a trnL reference library to aid in the metabarcoding analysis of foraged plants from two semi-arid eastern South African savanna bioregions

Danielle Botha, Mornè du Plessis, Frances Siebert, Sandra Barnard

published 19 May 2023

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Evaluation of miniaturized Illumina DNA preparation protocols for SARS-CoV-2 whole genome sequencing

Sureshnee Pillay, James Emmanuel San, Derek Tshiabuila, Yeshnee Naidoo, Yusasha Pillay, Akhil Maharaj, Ugochukwu J. Anyaneji, Eduan Wilkinson, Houriiyah Tegally, Richard J. Lessells, Cheryl Baxter, Tulio de Oliveira, Jennifer Giandhari

published 26 Apr 2023

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Optimised protocol for monitoring SARS-CoV-2 in wastewater using reverse complement PCR-based whole-genome sequencing

Harry T. Child, Paul A. O’Neill, Karen Moore, William Rowe, Hubert Denise, David Bass, Matthew J. Wade, Matt Loose, Steve Paterson, Ronny van Aerle, Aaron R. Jeffries

published 14 Apr 2023

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Bioinformatic analysis predicts that ethanol exposure during early development causes alternative splicing alterations of genes involved in RNA post-transcriptional regulation

Camilo Fuentes-Beals, Montserrat Olivares-Costa, María Estela Andrés, Paola A. Haeger, Gonzalo Riadi, Carlos Oliva, Fernando Faunes

published 13 Apr 2023

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A novel eukaryotic RdRP-dependent small RNA pathway represses antiviral immunity by controlling an ERK pathway component in the black-legged tick

Canran Feng, Kyosuke Torimaru, Mandy Yu Theng Lim, Li-Ling Chak, Masami Shiimori, Kosuke Tsuji, Tetsuya Tanaka, Junko Iida, Katsutomo Okamura

published 30 Mar 2023

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Target-enriched enzymatic methyl sequencing: Flexible, scalable and inexpensive hybridization capture for quantifying DNA methylation

Dustin R. Rubenstein, Joseph Solomon

published 09 Mar 2023

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Preparation of noninfectious scRNAseq samples from SARS-CoV-2-infected epithelial cells

Raven M. Osborn, Justin Leach, Michelle Zanche, John M. Ashton, ChinYi Chu, Juilee Thakar, Stephen Dewhurst, Sonia Rosenberger, Martin Pavelka, Gloria S. Pryhuber, Thomas J. Mariani, Christopher S. Anderson

published 24 Feb 2023

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