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DNA libraries

Error-corrected ultradeep next-generation sequencing for detection of clonal haematopoiesis and haematological neoplasms – sensitivity, specificity and accuracy

Melinda L. Tursky, Crisbel M. Artuz, Melissa Rapadas, Gary A. Wittert, Timothy J. Molloy, David D. Ma

published 26 Feb 2025

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A new method for the reproducible development of aptamers (Neomers)

Cathal Meehan, Erika L. Hamilton, Chloé G. Mansour, Soizic Lecocq, Cole J. Drake, Yisi An, Emily Rodrigues, Gregory Penner

published 12 Feb 2025

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Next-generation sequencing protocol of hematopoietic stem cells (HSCs). Step-by-step overview and troubleshooting guide

Justyna Jarczak, Kamila Bujko, Katarzyna Brzeźniakiewicz-Janus, Mariusz Ratajczak, Magdalena Kucia

published 09 Jan 2025

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Formalin-free tissue embedding is less hazardous and results in better DNA quality

Maarten Niemantsverdriet, Ageeth Knol, Jose van der Starre-Gaal, Agnes Marije Hoogland

published 30 Dec 2024

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RecombiCraft library construction: A novel method for DNA library cloning and expansion using non-enzymatic single-step DNA recombination and liquid culture

Yuki Kawai-Harada, Mehrsa Mardikoraem, Katherine Lauro, Vasudha Nimmagadda, Quynh Tong, Kayla Bello, Daniel Woldring, Masako Harada

published 02 Dec 2024

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Comparative analysis of whole exome sequencing kits for the canine genome

Jinhee Jang, Yong-Jik Lee, Soohyun Ko, A. M. Abd El-Aty, Ibrahim Gecili, Ji Hoon Jeong, ChangHyuk Kwon, Tae Woo Jung

published 04 Nov 2024

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Spatial transcriptomic profiling of isolated microregions in tissue sections utilizing laser-induced forward transfer

Kaiqiang Ye, Wanqing Chang, Jitao Xu, Yunxia Guo, Qingyang Qin, Kaitong Dang, Xiaofeng Han, Xiaolei Zhu, Qinyu Ge, Qiannan Cui, Yun Xu, Xiangwei Zhao

published 25 Jul 2024

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A cell competition system with one gene expression from a single-copy gene in one cell

Yoshinori Hasegawa, Megumi Nakano, Tsutomu Hosouchi, Takashi Watanabe, Izumi Yamaguchi, Manabu Nakayama, Osamu Ohara

published 05 Jul 2024

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Exploring the potential of Oxford Nanopore Technologies sequencing for Mycobacterium tuberculosis sequencing: An assessment of R10 flowcells and V14 chemistry

Anzaan Dippenaar, Emilyn Costa Conceição, Felicia Wells, Johannes Loubser, Brendon Mann, Miguel De Diego Fuertes, Vincent Rennie, Robin Mark Warren, Annelies Van Rie

published 06 Jun 2024

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Improving DNA barcoding library of armored scale insects (Hemiptera: Diaspididae) in China

Minmin Niu, Yubo Liu, Linjia Xue, Bo Cai, Qing Zhao, Jiufeng Wei

published 30 May 2024

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NGS method for parallel processing of high quality, damaged or fragmented input material using target enrichment

Anna Lyander, Anna Gellerbring, Moa Hägglund, Keyvan Elhami, Valtteri Wirta

published 29 May 2024

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High-throughput DNA extraction and cost-effective miniaturized metagenome and amplicon library preparation of soil samples for DNA sequencing

Thomas Bygh Nymann Jensen, Sebastian Mølvang Dall, Simon Knutsson, Søren Michael Karst, Mads Albertsen

published 04 Apr 2024

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High-throughput sequencing of insect specimens with sub-optimal DNA preservation using a practical, plate-based Illumina-compatible Tn5 transposase library preparation method

Lauren Cobb, Erik de Muinck, Spyros Kollias, Morten Skage, Gregor D. Gilfillan, Markus A. K. Sydenham, Shuo-Wang Qiao, Bastiaan Star

published 22 Mar 2024

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