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DNA libraries

A Low-Cost Library Construction Protocol and Data Analysis Pipeline for Illumina-Based Strand-Specific Multiplex RNA-Seq

Lin Wang, Yaqing Si, [ ... ], Thomas P. Brutnell

Next-Generation Sequencing of RNA and DNA Isolated from Paired Fresh-Frozen and Formalin-Fixed Paraffin-Embedded Samples of Human Cancer and Normal Tissue

Jakob Hedegaard, Kasper Thorsen, [ ... ], Lars Dyrskjøt

Emulsion PCR: A High Efficient Way of PCR Amplification of Random DNA Libraries in Aptamer Selection

Keke Shao, Weifeng Ding, [ ... ], Huimin Wang

Systematic Comparison of Three Methods for Fragmentation of Long-Range PCR Products for Next Generation Sequencing

Ellen Knierim, Barbara Lucke, [ ... ], Dominik Seelow

Inexpensive Multiplexed Library Preparation for Megabase-Sized Genomes

Michael Baym, Sergey Kryazhimskiy, [ ... ], Roy Kishony

A Microfluidic Device for Preparing Next Generation DNA Sequencing Libraries and for Automating Other Laboratory Protocols That Require One or More Column Chromatography Steps

Swee Jin Tan, Huan Phan, [ ... ], William F. Burkholder

Multiplexed DNA Sequence Capture of Mitochondrial Genomes Using PCR Products

Tomislav Maricic, Mark Whitten, Svante Pääbo

Whole Genome Amplification and De novo Assembly of Single Bacterial Cells

Sébastien Rodrigue, Rex R. Malmstrom, [ ... ], Sallie W. Chisholm

Environmental Barcoding: A Next-Generation Sequencing Approach for Biomonitoring Applications Using River Benthos

Mehrdad Hajibabaei, Shadi Shokralla, [ ... ], Donald J. Baird

A Method for Selectively Enriching Microbial DNA from Contaminating Vertebrate Host DNA

George R. Feehery, Erbay Yigit, [ ... ], Sriharsa Pradhan

Unlocking Short Read Sequencing for Metagenomics

Sébastien Rodrigue, Arne C. Materna, [ ... ], Sallie W. Chisholm

Comparison of normalization approaches for gene expression studies completed with high-throughput sequencing

Farnoosh Abbas-Aghababazadeh, Qian Li, Brooke L. Fridley

Optimizing exosomal RNA isolation for RNA-Seq analyses of archival sera specimens

Emily N. Prendergast, Marcos Abraão de Souza Fonseca, [ ... ], Kate Lawrenson

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